FastQCFastQC Report
Fri 10 Feb 2017
ERR1630220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323433
Sequences flagged as poor quality0
Sequence length43
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201356.225400623931386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143004.421317552630684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115863.5821947667677696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55991.731115872530014No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA46991.4528511314553556No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA25710.7949096103366076No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22310.6897873748195144No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.5376693163653677No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.44615113485636904No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA14310.4424409383087069No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.3608166142601404No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA11660.3605074312145019No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.35092275679970814No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA10350.32000445223585716No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10100.31227487609489446No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10040.3104197778210634No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA8000.24734643651080748No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.24734643651080748No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7840.24239950778059136No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.22199342676844971No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.2207566945858957No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.2077710066690783No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA6520.20158734575630813No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6000.18550982738310562No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5620.17376087164884227No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5460.16881394291862611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5420.1675772107360721No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA5360.16572211246224103No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA5350.16541292941660252No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5320.16448538027968698No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5210.16108436677766338No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.15459152281925467No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA4850.14995377713467706No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA4510.13944155358296773No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4470.13820482140041368No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4430.13696808921785963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4340.13418544180711306No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA4330.13387625876147455No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.1292385130768969No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA4040.12490995043795779No Hit
GTACATGGGAAGATGGTATCAACGCAAAAAAAAAAAAAAAAAA4010.12398240130104227No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.11934465561646461No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.115325276023164No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.11285181165805593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG3360.10388550333453915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTC251.2316646E-437.020
ATTAGGT251.2316646E-437.019
GAATAAT200.001839981137.01
TAGCACT200.001839981137.04
CGTCATT251.2316646E-437.026
CTAGTAC352.3830427E-531.7142853
GTCATTG303.5938103E-430.83333427
TAGGTCG303.5938103E-430.83333421
CAATTAG303.5938103E-430.83333417
AGCGTAT250.005490576429.626
ATAGCAC250.005490576429.63
AAGTTCC250.005490576429.64
GAGCGTA250.005490576429.625
TACTGTG556.238388E-726.909097
GGTCGTC358.855563E-426.4285723
CATTGCG358.855563E-426.4285729
TAACCTT358.855563E-426.428575
GAGCGAT358.855563E-426.428577
ACTGTGC358.855563E-426.428578
CTGTGCG358.855563E-426.428579
Produced by FastQC (version 0.11.5)