FastQCFastQC Report
Fri 10 Feb 2017
ERR1630219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503524
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG60930.4052479375121381No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT50040.3328180993452715No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG49810.3312883598798556No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC49780.3310888286452361No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG45080.29982893522151954No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA37280.24795081422045806No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA36570.24322857500113068No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG36390.2420313875934139No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA36030.23963701277798025No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC35170.23391711738555554No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC34540.22972696145854674No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT33950.22580284717769722No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT32360.2152276917428654No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC31960.21256727528127253No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG30130.20039586996948502No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG30000.1995312346194673No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA29840.19846706803483016No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA29780.19806800556559123No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC29420.19567363075015765No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG28320.18835748548077716No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA28140.18716029807306034No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC24680.16414769568028179No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC23340.1552353005339456No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG22360.14871728020304298No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT21860.14539175962605186No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC21750.14466014509911382No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG21410.14239879110675985No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT21330.14186670781444127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.1413346245221227No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC20940.1392728017643882No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA20880.13887373929514926No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA20500.13634634365663603No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG20400.13568123954123779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20230.13455056254506081No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG20210.13441754172198117No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC19900.13235571896424667No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC19820.1318236356719281No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG19690.13095900032191038No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC19480.12956228167957412No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA19340.12863113591801661No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG19240.1279660318026184No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA19080.12690186521798122No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC18660.12410842793330867No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC18340.12198009476403436No Hit
GATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTTC17940.11931967830244147No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA17480.11626019937160963No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC17460.11612717854852998No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG17270.11486348072927337No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT17170.11419837661387515No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC17120.11386582455617603No Hit
GTACATGGGAAGCCGCTTTAGGAGCGAGGTTCGGAGCCATCGC16970.11286816838307868No Hit
GGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCG16650.11073983521380437No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA16560.11014124150994598No Hit
GTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCAGGACGATG16320.10854499163299022No Hit
GATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGG15930.10595108558293716No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA15900.10575155434831768No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC15700.10442134611752124No Hit
CTCTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCG15680.1042883252944416No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT15290.10169441924438852No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG15220.10122884636360976No Hit
CTGCAGAAGTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGG15090.10036421101359207No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15070.10023119019051242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.020.2971441
TAATGGT801.6172107E-518.54
CGAACTA650.001580239717.07692329
ACGGACC2850.016.8771938
TATAGGG1708.54925E-1116.323532
GTATAGG2050.016.2439041
GTGCGTA1851.8189894E-1116.00000215
TCTTATA10550.015.95734737
CGGACCA3150.015.8571439
AAAACGC700.002592992315.85714215
ATATAAC700.002592992315.8571423
TAGGAGT2250.015.6222224
CGCAAGA3200.015.6093752
CGTTATT2055.456968E-1215.3414642
GCAAGAC3950.014.9873433
GCCTTAC750.00410518914.81
GATCGGA750.00410518914.817
ATAGCAC750.00410518914.83
GACGGAC3250.014.87
AATACGA1508.105417E-814.85
Produced by FastQC (version 0.11.5)