##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630219.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1503524
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56321282533568	31.0	31.0	34.0	30.0	34.0
2	31.761544212130968	31.0	31.0	34.0	30.0	34.0
3	31.916555372578024	33.0	31.0	34.0	30.0	34.0
4	35.62263322700536	37.0	35.0	37.0	33.0	37.0
5	35.515496260784666	37.0	35.0	37.0	33.0	37.0
6	35.561678430141455	37.0	35.0	37.0	33.0	37.0
7	35.52618714433557	37.0	35.0	37.0	33.0	37.0
8	35.50466903089009	37.0	35.0	37.0	33.0	37.0
9	37.19344287154711	39.0	37.0	39.0	34.0	39.0
10	37.06032893389131	39.0	37.0	39.0	33.0	39.0
11	37.15068000244759	39.0	37.0	39.0	33.0	39.0
12	36.982364764380215	39.0	37.0	39.0	33.0	39.0
13	37.05186082829406	39.0	37.0	39.0	33.0	39.0
14	38.21887379250348	40.0	38.0	41.0	33.0	41.0
15	38.18372037958822	40.0	38.0	41.0	33.0	41.0
16	38.17768588994921	40.0	38.0	41.0	33.0	41.0
17	38.087347458371134	40.0	37.0	41.0	33.0	41.0
18	38.04514992776969	40.0	37.0	41.0	33.0	41.0
19	38.03042252734243	40.0	37.0	41.0	33.0	41.0
20	37.98223041334891	40.0	37.0	41.0	33.0	41.0
21	37.92911120806851	40.0	37.0	41.0	33.0	41.0
22	37.91395348527858	40.0	37.0	41.0	33.0	41.0
23	37.868477656492345	40.0	37.0	41.0	33.0	41.0
24	37.839831622242144	40.0	37.0	41.0	33.0	41.0
25	37.809565394366835	40.0	37.0	41.0	33.0	41.0
26	37.62421750500823	39.0	37.0	41.0	32.0	41.0
27	37.51305266826469	39.0	37.0	41.0	32.0	41.0
28	37.410079918910505	39.0	37.0	41.0	32.0	41.0
29	37.33161492600052	39.0	36.0	41.0	31.0	41.0
30	37.25662842761406	39.0	36.0	40.0	31.0	41.0
31	37.09922688297626	39.0	36.0	40.0	31.0	41.0
32	37.01127817048481	39.0	36.0	40.0	31.0	41.0
33	36.9511567490775	39.0	36.0	40.0	31.0	41.0
34	36.80161540487548	39.0	36.0	40.0	30.0	41.0
35	36.737191424945664	39.0	35.0	40.0	30.0	41.0
36	36.61290541421354	39.0	35.0	40.0	30.0	41.0
37	36.497497213213755	39.0	35.0	40.0	30.0	41.0
38	36.4242685850043	39.0	35.0	40.0	30.0	41.0
39	36.24529837900825	38.0	35.0	40.0	30.0	41.0
40	36.143843397245405	38.0	35.0	40.0	29.0	41.0
41	35.996325299762425	38.0	35.0	40.0	29.0	41.0
42	35.929122514838475	38.0	35.0	40.0	29.0	41.0
43	34.63245282416509	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	8.0
11	1.0
12	0.0
13	1.0
14	4.0
15	6.0
16	15.0
17	37.0
18	84.0
19	186.0
20	338.0
21	706.0
22	1263.0
23	2172.0
24	3582.0
25	5495.0
26	8148.0
27	11899.0
28	16401.0
29	22710.0
30	29500.0
31	38439.0
32	48247.0
33	62151.0
34	81649.0
35	108833.0
36	152758.0
37	229679.0
38	380359.0
39	298853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.564636148142625	18.36658410507581	11.336633136551196	25.732146610230366
2	19.72958196876139	19.874641176329742	33.62114605420333	26.77463080070554
3	21.169199826540847	20.332099786900642	27.963903469449107	30.534796917109404
4	14.192324166425013	15.210997629568931	32.96548641724375	37.6311917867623
5	16.364421186492535	34.60842660310045	32.815704970456075	16.211447239950942
6	38.25858449881744	32.78743804555165	14.914361194101325	14.03961626152958
7	33.605782149137625	27.957718001175902	18.903988230317573	19.532511619368893
8	28.743139451049665	32.79934340921728	18.70365887075963	19.753858268973428
9	27.003293595579454	12.904283536544812	18.071144857015916	42.02127801085982
10	17.521569326462362	25.487986889467678	31.81592046418946	25.174523319880493
11	38.925484395327246	20.814167249741274	19.01818660693145	21.242161748000033
12	22.810876314578284	23.499658136484687	27.30185883298171	26.387606715955314
13	32.26114115903704	17.752493475328627	23.852229828057283	26.134135537577052
14	24.47968905052397	19.042595927966563	23.075853794152938	33.401861227356534
15	25.65526057449033	26.6324315408334	21.350440697986866	26.361867186689402
16	28.573803943269276	23.8844873776541	21.982821690907496	25.558886988169128
17	25.766599003407993	24.65846903674301	22.810743293755202	26.76418866609379
18	25.24356112705883	22.754874548061753	25.330889297410618	26.6706750274688
19	28.461800410236222	23.72705723353934	23.43062032930635	24.380522026918094
20	27.81644988706532	23.421707934160015	23.28010726799173	25.481734910782933
21	26.30067760807277	23.832010662949177	23.417251736586845	26.45005999239121
22	27.79662978442645	22.392525825992802	23.669791769203552	26.14105262037719
23	24.285744690473845	23.563774173209072	24.53954842090981	27.610932715407273
24	25.972714768769904	24.229144330253458	23.410733716255944	26.387407184720697
25	26.214679645951776	23.807002748210206	23.97001976689431	26.008297838943705
26	27.45403465458483	24.0641985096347	22.383546920434924	26.09821991534555
27	27.429558823138173	23.071796659049006	22.182751987996202	27.315892529816615
28	26.146706005358077	24.025090387649286	23.96975372524815	25.858449881744487
29	26.834623191914464	24.30310390788574	24.31128468850514	24.55098821169466
30	26.953477297336125	23.227298001229112	22.810743293755202	27.008481407679557
31	25.462380381024847	22.937578648561647	24.073909029719513	27.526131940694
32	24.96900614822244	24.008595805587408	23.427228298317818	27.595169747872333
33	26.03909215948665	22.618727735639737	24.207927508972254	27.134252595901366
34	27.35007888134809	23.010008486728513	24.28774000282004	25.352172629103357
35	27.78046775442228	22.98114296812023	22.87998063216816	26.358408645289334
36	25.489184076875393	23.331120753642775	22.80502339836278	28.374671771119054
37	25.70760426837217	22.98094343688561	25.56939563319242	25.7420566615498
38	25.51705193931058	22.322822914699067	23.908564146631512	28.251560999358837
39	24.943133598133453	23.498460949076968	25.682795884867815	25.875609567921764
40	27.21073956917216	22.12694975271429	24.38590937025282	26.27640130786073
41	24.221761674572537	23.394638196663305	25.98561778860863	26.39798234015553
42	24.8879299565554	24.166824074640644	24.744600019687084	26.200645949116875
43	25.54585094750732	22.152290219510963	24.698707835724605	27.60315099725711
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	34.5
2	48.0
3	172.0
4	296.0
5	296.0
6	400.0
7	504.0
8	501.0
9	498.0
10	643.5
11	789.0
12	789.0
13	1437.0
14	2085.0
15	3274.5
16	4464.0
17	4192.5
18	3921.0
19	3921.0
20	4861.5
21	5802.0
22	5790.5
23	5779.0
24	6999.0
25	8219.0
26	8219.0
27	9485.5
28	10752.0
29	13288.5
30	15825.0
31	19599.0
32	23373.0
33	23373.0
34	34913.0
35	46453.0
36	51000.0
37	55547.0
38	57219.5
39	58892.0
40	58892.0
41	62502.0
42	66112.0
43	73985.0
44	81858.0
45	84185.0
46	86512.0
47	86512.0
48	88864.0
49	91216.0
50	97947.5
51	104679.0
52	119163.5
53	133648.0
54	133648.0
55	131336.5
56	129025.0
57	115900.0
58	102775.0
59	105706.5
60	108638.0
61	108638.0
62	104025.0
63	99412.0
64	79832.5
65	60253.0
66	54928.5
67	49604.0
68	49604.0
69	46684.0
70	43764.0
71	46594.5
72	49425.0
73	44600.0
74	39775.0
75	39775.0
76	22945.0
77	6115.0
78	4592.5
79	3070.0
80	2431.5
81	1793.0
82	1793.0
83	1344.0
84	895.0
85	787.5
86	680.0
87	590.0
88	500.0
89	500.0
90	456.5
91	413.0
92	241.0
93	69.0
94	44.5
95	20.0
96	20.0
97	12.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1503524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.95553834367817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59609562238336	31.396642933685076
2	10.492861185518928	8.1751011249475
3	3.295356027669375	3.85117104275836
4	1.5031897751847458	2.3423026770013733
5	0.8499453055798316	1.6555038470772194
6	0.5782493150666791	1.3515608019171395
7	0.3891399939785221	1.0611410569542308
8	0.2721481769841445	0.8481342994934363
9	0.2277002319206774	0.7983166603905325
>10	1.432933584872848	10.831972970840916
>50	0.172701644473191	4.698478877710696
>100	0.14617159576420757	11.692857629401814
>500	0.023215486092802936	6.435911377595048
>1k	0.01994812138344549	14.117511114611988
>5k	3.4393312730078427E-4	0.7433935856146666
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	6093	0.4052479375121381	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	5004	0.3328180993452715	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	4981	0.3312883598798556	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	4978	0.3310888286452361	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	4508	0.29982893522151954	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	3728	0.24795081422045806	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	3657	0.24322857500113068	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	3639	0.2420313875934139	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	3603	0.23963701277798025	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	3517	0.23391711738555554	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	3454	0.22972696145854674	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	3395	0.22580284717769722	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	3236	0.2152276917428654	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	3196	0.21256727528127253	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	3013	0.20039586996948502	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	3000	0.1995312346194673	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2984	0.19846706803483016	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	2978	0.19806800556559123	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	2942	0.19567363075015765	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	2832	0.18835748548077716	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	2814	0.18716029807306034	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	2468	0.16414769568028179	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	2334	0.1552353005339456	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	2236	0.14871728020304298	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	2186	0.14539175962605186	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	2175	0.14466014509911382	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	2141	0.14239879110675985	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	2133	0.14186670781444127	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.1413346245221227	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	2094	0.1392728017643882	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	2088	0.13887373929514926	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	2050	0.13634634365663603	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	2040	0.13568123954123779	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2023	0.13455056254506081	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	2021	0.13441754172198117	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1990	0.13235571896424667	No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC	1982	0.1318236356719281	No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG	1969	0.13095900032191038	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1948	0.12956228167957412	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1934	0.12863113591801661	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	1924	0.1279660318026184	No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA	1908	0.12690186521798122	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	1866	0.12410842793330867	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	1834	0.12198009476403436	No Hit
GATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTTC	1794	0.11931967830244147	No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA	1748	0.11626019937160963	No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC	1746	0.11612717854852998	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	1727	0.11486348072927337	No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT	1717	0.11419837661387515	No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC	1712	0.11386582455617603	No Hit
GTACATGGGAAGCCGCTTTAGGAGCGAGGTTCGGAGCCATCGC	1697	0.11286816838307868	No Hit
GGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCG	1665	0.11073983521380437	No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA	1656	0.11014124150994598	No Hit
GTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCAGGACGATG	1632	0.10854499163299022	No Hit
GATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGG	1593	0.10595108558293716	No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA	1590	0.10575155434831768	No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC	1570	0.10442134611752124	No Hit
CTCTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCG	1568	0.1042883252944416	No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT	1529	0.10169441924438852	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1522	0.10122884636360976	No Hit
CTGCAGAAGTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGG	1509	0.10036421101359207	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1507	0.10023119019051242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	6.651041153982244E-5	0.0	0.0	0.0
10	0.0	6.651041153982244E-5	0.0	0.0	0.0
11	0.0	6.651041153982244E-5	0.0	0.0	0.0
12	0.0	6.651041153982244E-5	0.0	0.0	0.0
13	0.0	6.651041153982244E-5	0.0	0.0	0.0
14	0.0	6.651041153982244E-5	0.0	1.3302082307964488E-4	0.0
15	0.0	6.651041153982244E-5	0.0	2.6604164615928977E-4	0.0
16	0.0	6.651041153982244E-5	0.0	5.320832923185795E-4	0.0
17	0.0	6.651041153982244E-5	0.0	7.981249384778693E-4	0.0
18	0.0	6.651041153982244E-5	0.0	9.311457615575142E-4	0.0
19	0.0	6.651041153982244E-5	0.0	9.976561730973366E-4	0.0
20	0.0	6.651041153982244E-5	0.0	0.0013967186423362713	0.0
21	0.0	6.651041153982244E-5	0.0	0.0017292707000353836	0.0
22	6.651041153982244E-5	6.651041153982244E-5	0.0	0.0024608852269734304	0.0
23	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.00299296851929201	0.0
24	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.004522707984707926	0.0
25	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.005387343334725618	0.0
26	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.006651041153982244	0.0
27	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.0130360406618052	0.0
28	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.03830999704693773	0.0
29	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.0732279631053445	0.0
30	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.11519603278697248	0.0
31	6.651041153982244E-5	1.3302082307964488E-4	0.0	0.30149169551001515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	875	0.0	20.297144	1
TAATGGT	80	1.6172107E-5	18.5	4
CGAACTA	65	0.0015802397	17.076923	29
ACGGACC	285	0.0	16.877193	8
TATAGGG	170	8.54925E-11	16.32353	2
GTATAGG	205	0.0	16.243904	1
GTGCGTA	185	1.8189894E-11	16.000002	15
TCTTATA	1055	0.0	15.957347	37
CGGACCA	315	0.0	15.857143	9
AAAACGC	70	0.0025929923	15.857142	15
ATATAAC	70	0.0025929923	15.857142	3
TAGGAGT	225	0.0	15.622222	4
CGCAAGA	320	0.0	15.609375	2
CGTTATT	205	5.456968E-12	15.341464	2
GCAAGAC	395	0.0	14.987343	3
GCCTTAC	75	0.004105189	14.8	1
GATCGGA	75	0.004105189	14.8	17
ATAGCAC	75	0.004105189	14.8	3
GACGGAC	325	0.0	14.8	7
AATACGA	150	8.105417E-8	14.8	5
>>END_MODULE