FastQCFastQC Report
Fri 10 Feb 2017
ERR1630218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104591
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA19380.17544955553684577No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT17610.15942552492279946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.023.746271
CTAGAAC4050.022.3827153
CGCGGAA502.7020098E-422.233
GTCTAGA3750.022.1999991
TCTAGAC3450.021.4492743
ACTGATC3500.021.1428598
TAGAACT4600.020.9130444
GTTCTAG3900.020.8717961
AATACTA1101.7553248E-920.1818185
CTAGACA3600.020.0416664
GTATATG1153.0504452E-919.3043481
TACCGTC1153.0504452E-919.3043487
GACGGAC1254.129106E-1019.247
TAATACT1306.9667294E-1018.54
TCCATAC609.236503E-418.53
GGATAGA1601.8189894E-1218.51
GTATAGG801.6165779E-518.51
TCGCGGA609.236503E-418.532
TAGACAT4050.018.2716055
TCTAGAA5300.018.1509442
Produced by FastQC (version 0.11.5)