##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1104591
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.985960414307197	33.0	31.0	34.0	30.0	34.0
2	32.209884020420226	33.0	31.0	34.0	30.0	34.0
3	32.34726428153045	34.0	31.0	34.0	30.0	34.0
4	35.96415053173528	37.0	35.0	37.0	35.0	37.0
5	35.89206502678367	37.0	35.0	37.0	35.0	37.0
6	35.94371581879628	37.0	35.0	37.0	35.0	37.0
7	35.926918651337914	37.0	35.0	37.0	35.0	37.0
8	35.923742815213956	37.0	35.0	37.0	35.0	37.0
9	37.70753156598234	39.0	37.0	39.0	35.0	39.0
10	37.60525660629138	39.0	37.0	39.0	35.0	39.0
11	37.66764621475279	39.0	37.0	39.0	35.0	39.0
12	37.59268724804023	39.0	37.0	39.0	35.0	39.0
13	37.59748721472473	39.0	37.0	39.0	35.0	39.0
14	38.870988447307646	40.0	38.0	41.0	36.0	41.0
15	38.88150365157782	40.0	38.0	41.0	36.0	41.0
16	38.85813119969292	40.0	38.0	41.0	35.0	41.0
17	38.822537934855525	40.0	38.0	41.0	35.0	41.0
18	38.78783006560799	40.0	38.0	41.0	35.0	41.0
19	38.802985901568995	40.0	38.0	41.0	35.0	41.0
20	38.77854518097649	40.0	38.0	41.0	35.0	41.0
21	38.727184994264846	40.0	38.0	41.0	35.0	41.0
22	38.689966693554446	40.0	38.0	41.0	34.0	41.0
23	38.6443262709908	40.0	38.0	41.0	34.0	41.0
24	38.618658852009474	40.0	38.0	41.0	34.0	41.0
25	38.60544038472158	40.0	38.0	41.0	34.0	41.0
26	38.48357717924553	40.0	38.0	41.0	34.0	41.0
27	38.369436289088	40.0	38.0	41.0	34.0	41.0
28	38.312337326666615	40.0	38.0	41.0	34.0	41.0
29	38.27196944389371	40.0	38.0	41.0	34.0	41.0
30	38.22530149168335	40.0	38.0	41.0	34.0	41.0
31	38.19768312434195	40.0	38.0	41.0	34.0	41.0
32	38.1178608190724	40.0	38.0	41.0	33.0	41.0
33	38.049830208647364	40.0	38.0	41.0	33.0	41.0
34	38.00579309445759	40.0	38.0	41.0	33.0	41.0
35	37.91301576782719	40.0	37.0	41.0	33.0	41.0
36	37.84822346008613	40.0	37.0	41.0	33.0	41.0
37	37.80913568913743	40.0	37.0	41.0	33.0	41.0
38	37.727309022072426	40.0	37.0	41.0	33.0	41.0
39	37.66440519613142	40.0	37.0	41.0	33.0	41.0
40	37.578392364232556	40.0	37.0	41.0	32.0	41.0
41	37.48621888101569	40.0	37.0	41.0	32.0	41.0
42	37.45504173037803	40.0	37.0	41.0	32.0	41.0
43	36.2553298008041	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	0.0
13	2.0
14	1.0
15	2.0
16	7.0
17	7.0
18	13.0
19	40.0
20	96.0
21	195.0
22	389.0
23	664.0
24	1215.0
25	1808.0
26	2905.0
27	4435.0
28	6418.0
29	9300.0
30	13012.0
31	18056.0
32	23853.0
33	32151.0
34	43997.0
35	61611.0
36	88216.0
37	136171.0
38	266667.0
39	393355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80299223875625	18.75680681808923	11.620862382547024	26.819338560607502
2	19.26903261026027	20.698611522273854	33.311334240456425	26.721021627009456
3	20.222417166172818	21.358312714841965	29.055913003093455	29.363357115891763
4	14.79660797525962	14.868127659921186	34.14521755111168	36.19004681370752
5	15.844688214913935	35.175825260209436	33.42277820478349	15.556708320093138
6	36.52990111271955	34.61362622002171	14.666695636665516	14.189777030593223
7	30.882833555587542	29.35919267855704	20.271575632971842	19.486398132883572
8	27.70056971313364	32.675533296939776	19.726758592094267	19.89713839783232
9	26.829296997712277	13.713673205738594	18.731458069095257	40.725571727453875
10	17.04649051096741	26.008631249032447	32.70169682715141	24.243181412848738
11	37.72762950268471	20.821915079880245	20.685122366559206	20.765333050875846
12	22.885574841728747	24.737210424491963	28.296717970724007	24.080496763055283
13	31.228572385616033	18.765135692758676	24.21647469515866	25.789817226466628
14	23.725252152154056	19.37939019963045	23.887574676961883	33.00778297125361
15	26.08666918343532	26.55688847727349	21.401767713117344	25.95467462617385
16	26.14497130612145	25.33390186956077	22.765983065225047	25.75514375909273
17	24.907318636490793	25.22743712378609	24.079500919344806	25.785743320378312
18	25.421083459850752	24.120149448981568	24.811355515299326	25.64741157586835
19	25.46906502044648	24.372912688950027	24.32321103467256	25.83481125593093
20	25.834901787177333	24.393101156898798	24.398351969190408	25.37364508673346
21	26.377455546894733	24.18524141514823	24.528083245291697	24.90921979266534
22	26.287286425473322	24.14522660423632	24.21973382002931	25.34775315026105
23	25.672669793615917	24.259477037202004	24.59960292995326	25.46825023922882
24	25.578245703613373	24.43737093639184	24.029165546342494	25.955217813652293
25	26.230885458961733	24.087196075289405	24.24589735024095	25.436021115507913
26	26.376640765677067	24.08674341905737	23.98027867328269	25.556337141982873
27	25.67393723106562	24.18641832135152	24.230959694583788	25.908684752999072
28	25.550543142212817	23.668398529410435	25.139350221032036	25.64170810734471
29	25.173752094666714	24.28989553599477	24.682348489169296	25.85400388016922
30	24.721095862631508	25.28429074652971	24.764550860906887	25.230062529931896
31	26.055526434671293	24.50563149618275	24.03839973347601	25.400442335669943
32	25.46779758299678	24.572262493538332	23.889837958122058	26.07010196534283
33	25.137449064857492	24.090545731406465	25.095352035278218	25.676653168457825
34	26.13220640037806	23.66242346714757	24.642514740750197	25.562855391724177
35	25.241922123211214	24.156361947544386	25.39238505473972	25.209330874504683
36	25.546288173631687	23.91328555094148	24.973315915121525	25.567110360305307
37	25.73468369740474	23.60674675060724	25.044564006043867	25.614005545944156
38	24.856802200995663	23.551160565313314	25.832276381031534	25.75976085265949
39	25.202903156009782	23.494488005062507	25.817791381606405	25.484817457321306
40	24.37101153277548	23.840317366337406	26.403981202091998	25.38468989879512
41	24.891204074630338	23.044909835405143	26.496142010934363	25.56774407903016
42	24.77985064154968	23.199808798007588	26.65936984820626	25.360970712236476
43	23.89671833284899	22.66096681939288	26.997322991043742	26.444991856714385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	19.0
2	21.0
3	46.5
4	72.0
5	72.0
6	95.5
7	119.0
8	117.5
9	116.0
10	173.5
11	231.0
12	231.0
13	379.0
14	527.0
15	794.5
16	1062.0
17	1287.0
18	1512.0
19	1512.0
20	2269.5
21	3027.0
22	3884.0
23	4741.0
24	5888.5
25	7036.0
26	7036.0
27	8503.5
28	9971.0
29	12361.0
30	14751.0
31	17808.0
32	20865.0
33	20865.0
34	25950.5
35	31036.0
36	36578.5
37	42121.0
38	46190.5
39	50260.0
40	50260.0
41	55511.0
42	60762.0
43	66320.0
44	71878.0
45	76776.0
46	81674.0
47	81674.0
48	84089.5
49	86505.0
50	88992.5
51	91480.0
52	92830.5
53	94181.0
54	94181.0
55	88307.5
56	82434.0
57	79264.5
58	76095.0
59	73604.5
60	71114.0
61	71114.0
62	66838.5
63	62563.0
64	56101.5
65	49640.0
66	42980.0
67	36320.0
68	36320.0
69	28879.0
70	21438.0
71	17786.5
72	14135.0
73	10889.0
74	7643.0
75	7643.0
76	5852.0
77	4061.0
78	3212.0
79	2363.0
80	1915.0
81	1467.0
82	1467.0
83	1047.0
84	627.0
85	490.5
86	354.0
87	282.0
88	210.0
89	210.0
90	167.0
91	124.0
92	74.0
93	24.0
94	16.0
95	8.0
96	8.0
97	6.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1104591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.83957678084782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.99714680234182	47.462047050223596
2	8.391924101063534	9.372029803607692
3	2.4582701041797406	4.118062866912221
4	1.1435894463740368	2.5543020278628115
5	0.6300681104629564	1.7591368315679983
6	0.419115989026892	1.4041955669608865
7	0.2800833600321126	1.0947815401285694
8	0.20588341299970409	0.9197154118479982
9	0.15882948112962128	0.7982073905939736
>10	1.0658015733261128	11.651551415656506
>50	0.13948412168403676	5.48900798449889
>100	0.10410153585996035	10.97071374785214
>500	0.005213221960652083	1.9745955522209382
>1k	4.887395588111328E-4	0.4316528100658045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	1938	0.17544955553684577	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	1761	0.15942552492279946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.053124640704115E-5	0.0	0.0	0.0
6	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	0.0
7	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	0.0
8	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
9	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
10	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
11	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
12	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
13	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
14	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
15	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
16	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
17	9.053124640704115E-5	9.053124640704115E-5	0.0	0.0	9.053124640704115E-5
18	9.053124640704115E-5	9.053124640704115E-5	0.0	1.810624928140823E-4	9.053124640704115E-5
19	9.053124640704115E-5	9.053124640704115E-5	0.0	3.621249856281646E-4	9.053124640704115E-5
20	1.810624928140823E-4	9.053124640704115E-5	0.0	4.526562320352058E-4	1.810624928140823E-4
21	1.810624928140823E-4	9.053124640704115E-5	0.0	7.242499712563292E-4	1.810624928140823E-4
22	1.810624928140823E-4	9.053124640704115E-5	0.0	9.053124640704116E-4	1.810624928140823E-4
23	1.810624928140823E-4	9.053124640704115E-5	0.0	0.002263281160176029	1.810624928140823E-4
24	1.810624928140823E-4	9.053124640704115E-5	0.0	0.002896999885025317	1.810624928140823E-4
25	1.810624928140823E-4	9.053124640704115E-5	0.0	0.003440187363467564	1.810624928140823E-4
26	1.810624928140823E-4	9.053124640704115E-5	0.0	0.005069749798794305	1.810624928140823E-4
27	2.715937392211235E-4	1.810624928140823E-4	0.0	0.01412287443949842	1.810624928140823E-4
28	2.715937392211235E-4	1.810624928140823E-4	0.0	0.03675568604125871	1.810624928140823E-4
29	2.715937392211235E-4	1.810624928140823E-4	0.0	0.0732397783432963	1.810624928140823E-4
30	2.715937392211235E-4	1.810624928140823E-4	0.0	0.12493312004171679	1.810624928140823E-4
31	2.715937392211235E-4	1.810624928140823E-4	0.0	0.29142008218426546	1.810624928140823E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	335	0.0	23.74627	1
CTAGAAC	405	0.0	22.382715	3
CGCGGAA	50	2.7020098E-4	22.2	33
GTCTAGA	375	0.0	22.199999	1
TCTAGAC	345	0.0	21.449274	3
ACTGATC	350	0.0	21.142859	8
TAGAACT	460	0.0	20.913044	4
GTTCTAG	390	0.0	20.871796	1
AATACTA	110	1.7553248E-9	20.181818	5
CTAGACA	360	0.0	20.041666	4
GTATATG	115	3.0504452E-9	19.304348	1
TACCGTC	115	3.0504452E-9	19.304348	7
GACGGAC	125	4.129106E-10	19.24	7
TAATACT	130	6.9667294E-10	18.5	4
TCCATAC	60	9.236503E-4	18.5	3
GGATAGA	160	1.8189894E-12	18.5	1
GTATAGG	80	1.6165779E-5	18.5	1
TCGCGGA	60	9.236503E-4	18.5	32
TAGACAT	405	0.0	18.271605	5
TCTAGAA	530	0.0	18.150944	2
>>END_MODULE