FastQCFastQC Report
Fri 10 Feb 2017
ERR1630217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455509
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67600.27529933712317894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57560.23441168409482513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46760.1904289497615362No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT34560.14074474986652463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.12807935136869789No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA29230.11903845597796628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA7200.020.5555574
TCTAGAC7150.020.1818183
ATAACTC759.272806E-619.7333343
GGTATCA29350.018.9097121
TTTAGCG609.2408917E-418.526
GTTCTAG8150.018.3865031
ACTGATC6900.018.2318848
ACATGTA6800.018.2279428
GTCTAGA7500.017.5133321
CTAGAAC7550.016.6622523
ACGGAGT7000.016.6526
TAGAACT8000.016.418754
GCCTCTA7700.016.33766225
TAGACAT9400.016.3351085
CTCTACC8650.016.2543351
AACGGAG7100.016.1549325
CAACGGA6950.015.97122324
AAGACGG4200.015.8571425
GACATGT8200.015.7926827
TCTAGTT8300.015.60240928
Produced by FastQC (version 0.11.5)