##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2455509
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.892086732323115	33.0	31.0	34.0	30.0	34.0
2	32.091862420378014	33.0	31.0	34.0	30.0	34.0
3	32.214481803976284	34.0	31.0	34.0	30.0	34.0
4	35.83891649348465	37.0	35.0	37.0	35.0	37.0
5	35.763995570775755	37.0	35.0	37.0	35.0	37.0
6	35.82103995546341	37.0	35.0	37.0	35.0	37.0
7	35.79924813959143	37.0	35.0	37.0	35.0	37.0
8	35.80245399222727	37.0	35.0	37.0	35.0	37.0
9	37.55715373065218	39.0	37.0	39.0	35.0	39.0
10	37.45137729081832	39.0	37.0	39.0	35.0	39.0
11	37.51790891420068	39.0	37.0	39.0	35.0	39.0
12	37.4442305037367	39.0	37.0	39.0	35.0	39.0
13	37.44708123651756	39.0	37.0	39.0	35.0	39.0
14	38.68967126571314	40.0	38.0	41.0	35.0	41.0
15	38.68890971281311	40.0	38.0	41.0	35.0	41.0
16	38.65420041221596	40.0	38.0	41.0	34.0	41.0
17	38.623979386758506	40.0	38.0	41.0	34.0	41.0
18	38.58679361386987	40.0	38.0	41.0	34.0	41.0
19	38.6040894168989	40.0	38.0	41.0	34.0	41.0
20	38.58270362682442	40.0	38.0	41.0	34.0	41.0
21	38.521264226683755	40.0	38.0	41.0	34.0	41.0
22	38.4765358221045	40.0	38.0	41.0	34.0	41.0
23	38.417964666388926	40.0	38.0	41.0	34.0	41.0
24	38.37943456937034	40.0	38.0	41.0	34.0	41.0
25	38.356170553640816	40.0	38.0	41.0	34.0	41.0
26	38.19304307172158	40.0	38.0	41.0	33.0	41.0
27	38.0708447820798	40.0	38.0	41.0	33.0	41.0
28	37.994881712915735	40.0	37.0	41.0	33.0	41.0
29	37.94832517412887	40.0	37.0	41.0	33.0	41.0
30	37.87357977510976	40.0	37.0	41.0	33.0	41.0
31	37.81929897222938	40.0	37.0	41.0	33.0	41.0
32	37.72089859984223	40.0	37.0	41.0	33.0	41.0
33	37.61834796777369	40.0	37.0	41.0	32.0	41.0
34	37.558065150646975	40.0	37.0	41.0	32.0	41.0
35	37.44070903425725	40.0	37.0	41.0	32.0	41.0
36	37.351906671895726	40.0	36.0	41.0	31.0	41.0
37	37.284893681920934	40.0	36.0	41.0	31.0	41.0
38	37.16461882241116	39.0	36.0	41.0	31.0	41.0
39	37.08529107407059	39.0	36.0	41.0	31.0	41.0
40	36.967243044110205	39.0	36.0	41.0	31.0	41.0
41	36.84669532874854	39.0	35.0	41.0	30.0	41.0
42	36.791336134381915	39.0	35.0	41.0	30.0	41.0
43	35.54315459646045	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	4.0
12	6.0
13	5.0
14	6.0
15	5.0
16	15.0
17	36.0
18	62.0
19	184.0
20	379.0
21	732.0
22	1364.0
23	2396.0
24	4086.0
25	6503.0
26	9876.0
27	14483.0
28	20779.0
29	28236.0
30	37905.0
31	49346.0
32	63840.0
33	82550.0
34	108839.0
35	144608.0
36	202664.0
37	307918.0
38	569155.0
39	799521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37206664687444	18.436299765140344	11.820156228301341	26.371477359683876
2	19.649449462412885	20.616295847419007	33.54852293353435	26.185731756633757
3	20.602734504332908	21.687153254172557	28.736689623210506	28.97342261828403
4	15.449912828664036	15.06066562981443	32.726208700517894	36.76321284100364
5	16.072635042266185	35.01196696896652	32.63384495841799	16.281553030349308
6	36.27431216908592	33.11655546772583	15.11845405575789	15.490678307430352
7	30.740714043402	29.239192362968332	20.342136803408174	19.677956790221497
8	27.3671161457767	32.54001512517364	19.66301080549898	20.429857923550678
9	26.94671451010768	13.87284672953754	19.334443490127708	39.84599527022707
10	17.858130432427654	25.6903965735821	32.10279416609754	24.34867882789271
11	36.80959833582365	21.159116093649015	20.527230810394098	21.504054760133233
12	22.52575738879393	24.83529891358574	28.37582757790747	24.26311611971286
13	30.654255390633878	19.136154662841797	24.41176147185777	25.797828474666556
14	23.67044877457179	19.988279415795258	24.299809123077946	32.04146268655501
15	26.26502285269571	26.768665885565884	21.626839893480334	25.339471368258064
16	25.93059117274667	25.08917703009844	23.420561683952286	25.5596701132026
17	24.67622802441368	25.70408009093023	24.488568357924976	25.13112352673112
18	24.803452155948115	24.000522905841517	25.70489458601048	25.491130352199892
19	25.116096092500577	24.21774874374315	25.532506702276393	25.13364846147988
20	25.10534475744133	24.29317098817394	25.619006079798528	24.982478174586205
21	25.74631166084099	24.07036585897262	25.653866469233062	24.52945601095333
22	25.60670720408681	24.071831950117065	25.242342829938718	25.079118015857404
23	24.81839814067063	24.262749596926746	25.58206872790937	25.336783534493257
24	24.86567958007892	24.62906875926743	25.28913557229886	25.216116088354795
25	25.88994786824239	24.07598587502632	25.24356457255909	24.790501684172202
26	25.886241915627267	24.328031377608472	24.81025318986817	24.97547351689609
27	25.482211631071195	24.009115828938114	25.091457616323133	25.417214923667558
28	24.70286201353772	24.15116377093303	25.679930311800938	25.46604390372831
29	24.507383194278663	24.358534218363687	25.46624752749837	25.66783505985928
30	24.050695802784677	24.93450441435971	26.073290710805786	24.94150907204983
31	25.419128987106138	24.33487313628254	25.203002717562832	25.04299515904849
32	24.779017303540734	24.40365724580932	25.358326929365766	25.458998521284183
33	24.701151573869208	24.09952478284543	25.978564932973164	25.220758710312204
34	25.459202145054245	23.67350313112271	25.236763538639035	25.630531185184008
35	24.692599375526623	24.128724431472254	26.11389329055605	25.06478290244507
36	24.77918020255678	23.7389885355745	25.957265886624732	25.52456537524399
37	25.03521673103214	23.757518298650098	26.03505016678823	25.172214803529535
38	24.492681558080218	23.375886628800792	26.68978203704405	25.441649776074943
39	24.827805558847473	23.20814136702411	26.877319529270714	25.086733544857704
40	24.384027914375388	23.76232381962355	26.816232398252254	25.037415867748802
41	24.401987530894818	23.279613310315703	27.103016116006906	25.215383042782573
42	24.200399998533907	23.498468138377827	27.023969368469018	25.277162494619244
43	23.65631728492952	23.37902243485974	27.047060303993998	25.917599976216742
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	706.0
1	676.0
2	646.0
3	1221.0
4	1796.0
5	1796.0
6	2201.5
7	2607.0
8	2374.5
9	2142.0
10	3024.0
11	3906.0
12	3906.0
13	6471.0
14	9036.0
15	11626.0
16	14216.0
17	13282.5
18	12349.0
19	12349.0
20	14306.0
21	16263.0
22	14430.5
23	12598.0
24	14325.0
25	16052.0
26	16052.0
27	19449.5
28	22847.0
29	27610.5
30	32374.0
31	37877.5
32	43381.0
33	43381.0
34	53467.5
35	63554.0
36	75239.5
37	86925.0
38	97296.5
39	107668.0
40	107668.0
41	118763.0
42	129858.0
43	138964.0
44	148070.0
45	160575.0
46	173080.0
47	173080.0
48	182348.0
49	191616.0
50	197957.0
51	204298.0
52	205724.0
53	207150.0
54	207150.0
55	197667.5
56	188185.0
57	177975.5
58	167766.0
59	160262.5
60	152759.0
61	152759.0
62	142892.5
63	133026.0
64	119673.5
65	106321.0
66	93119.0
67	79917.0
68	79917.0
69	64269.5
70	48622.0
71	41006.0
72	33390.0
73	25918.5
74	18447.0
75	18447.0
76	14217.5
77	9988.0
78	7812.0
79	5636.0
80	4642.5
81	3649.0
82	3649.0
83	2693.5
84	1738.0
85	1477.0
86	1216.0
87	1045.0
88	874.0
89	874.0
90	771.0
91	668.0
92	386.5
93	105.0
94	78.0
95	51.0
96	51.0
97	30.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2455509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.53744027659299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.59638324337679	33.007515178947614
2	11.387745436669459	9.68811082794743
3	3.875524239440156	4.945646426270224
4	1.8575637890112133	3.16064034940105
5	1.1239101143733272	2.3904129683207107
6	0.7097235763990392	1.8113894546378468
7	0.5071340549779155	1.5100529203094655
8	0.3866343039780695	1.3157146891479385
9	0.2956352353803559	1.131800955178356
>10	1.8766102149134682	15.267817404729952
>50	0.20315586015092935	6.056127812907912
>100	0.1651940018041894	14.002465614433286
>500	0.012289603761141951	3.5236216128140874
>1k	0.0023043007052141106	1.6775144202875143
>5k	1.9202505876784255E-4	0.5111693646666633
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6760	0.27529933712317894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5756	0.23441168409482513	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4676	0.1904289497615362	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	3456	0.14074474986652463	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3145	0.12807935136869789	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	2923	0.11903845597796628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.072475401230458E-5	0.0
2	0.0	0.0	0.0	4.072475401230458E-5	0.0
3	0.0	0.0	0.0	4.072475401230458E-5	0.0
4	0.0	0.0	0.0	1.2217426203691372E-4	0.0
5	0.0	4.072475401230458E-5	0.0	1.2217426203691372E-4	0.0
6	0.0	4.072475401230458E-5	0.0	1.2217426203691372E-4	0.0
7	0.0	4.072475401230458E-5	0.0	1.2217426203691372E-4	0.0
8	0.0	4.072475401230458E-5	0.0	1.2217426203691372E-4	0.0
9	0.0	4.072475401230458E-5	0.0	1.2217426203691372E-4	0.0
10	0.0	4.072475401230458E-5	0.0	1.628990160492183E-4	0.0
11	0.0	4.072475401230458E-5	0.0	2.4434852407382744E-4	0.0
12	0.0	4.072475401230458E-5	0.0	3.665227861107412E-4	0.0
13	0.0	4.072475401230458E-5	0.0	4.0724754012304575E-4	0.0
14	0.0	4.072475401230458E-5	0.0	4.886970481476549E-4	0.0
15	0.0	4.072475401230458E-5	0.0	6.108713101845687E-4	0.0
16	0.0	4.072475401230458E-5	0.0	6.923208182091778E-4	0.0
17	0.0	4.072475401230458E-5	0.0	8.552198342583961E-4	0.0
18	0.0	4.072475401230458E-5	0.0	9.773940962953098E-4	0.0
19	0.0	4.072475401230458E-5	0.0	0.0010995683583322237	0.0
20	0.0	4.072475401230458E-5	0.0	0.0012624673743814418	0.0
21	0.0	4.072475401230458E-5	0.0	0.0017511644225290967	0.0
22	0.0	4.072475401230458E-5	0.0	0.00240276048672597	0.0
23	0.0	4.072475401230458E-5	0.0	0.003461604091045889	0.0
24	0.0	4.072475401230458E-5	0.0	0.006353061625919514	0.0
25	0.0	4.072475401230458E-5	0.0	0.00879654686665779	0.0
26	0.0	4.072475401230458E-5	0.0	0.014457287674368125	0.0
27	0.0	4.072475401230458E-5	0.0	0.03742604893730791	0.0
28	0.0	4.072475401230458E-5	0.0	0.08816909243663941	0.0
29	0.0	4.072475401230458E-5	0.0	0.15495768901681892	0.0
30	0.0	4.072475401230458E-5	0.0	0.24683273406857803	0.0
31	0.0	4.072475401230458E-5	0.0	0.4695971385158841	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	720	0.0	20.555557	4
TCTAGAC	715	0.0	20.181818	3
ATAACTC	75	9.272806E-6	19.733334	3
GGTATCA	2935	0.0	18.909712	1
TTTAGCG	60	9.2408917E-4	18.5	26
GTTCTAG	815	0.0	18.386503	1
ACTGATC	690	0.0	18.231884	8
ACATGTA	680	0.0	18.227942	8
GTCTAGA	750	0.0	17.513332	1
CTAGAAC	755	0.0	16.662252	3
ACGGAGT	700	0.0	16.65	26
TAGAACT	800	0.0	16.41875	4
GCCTCTA	770	0.0	16.337662	25
TAGACAT	940	0.0	16.335108	5
CTCTACC	865	0.0	16.254335	1
AACGGAG	710	0.0	16.15493	25
CAACGGA	695	0.0	15.971223	24
AAGACGG	420	0.0	15.857142	5
GACATGT	820	0.0	15.792682	7
TCTAGTT	830	0.0	15.602409	28
>>END_MODULE