FastQCFastQC Report
Fri 10 Feb 2017
ERR1630216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005344
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC55240.5494636661679982No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT28930.2877621988095617No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG23500.23375083553490147No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG20700.2058996721520196No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT19880.19774326001846132No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG19880.19774326001846132No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG18640.1854091733774708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16780.16690804341598497No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT16410.16322771111181844No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT16060.1597463156889582No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT15100.15019734538625584No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC14630.1455223286755578No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG14480.1440303020657606No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG14380.14303561765922906No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC14050.13975315911767514No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT13590.13517761084763028No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG13120.13050259413693224No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG12930.1286126937645224No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG12100.12035681319031098No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT11800.11737275997071649No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT11700.116378075564185No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC10890.10832113187127987No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA10650.10593388929560428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10600.10543654709233855No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG10340.10285036763535665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTT358.868618E-426.42857218
GTTTAGG952.8194336E-1023.3684221
ATAAGCG400.001931019423.1250023
TGCATCA10600.021.4669814
GACAGGC11350.020.700447
GCATCAG11400.020.6096515
GCTGCAT11050.020.5927612
ACACCGT450.003825478720.5555576
AGAACGT450.003825478720.55555727
CACGTAT555.142478E-420.1818181
ACCGTCC656.901033E-519.9230758
CGAGCGT851.2449909E-619.5882366
ATACCGC851.2449909E-619.58823627
CAGGACA13350.019.5393264
CTGCATC12000.019.11666713
CCAGGAC13350.018.7078653
TACTGTG1409.458745E-1118.57
ATACCGT609.235718E-418.56
TTTAGCG500.00703428518.49999826
ATAACGT500.00703428518.49999817
Produced by FastQC (version 0.11.5)