FastQCFastQC Report
Fri 10 Feb 2017
ERR1630215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742833
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48120.17543904422908724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.17317860766586957No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG46230.1685483585766979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36880.13445951685720567No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT36840.1343136822402239No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC31930.11641248300570978No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG31670.11546455799532819No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA31050.11320412143211052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.017.847061
CTAGACT2800.014.5357154
CGCAATA2301.8189894E-1214.47826136
ATAACGC2950.014.4237293
TTAACGG908.2791736E-414.38888835
CTAGCGG2450.014.3469429
CGGCCTT10450.014.1626824
TAACGGC1051.6569516E-414.09523936
ATCTCGC11000.013.95909111
TCTAGCG2405.456968E-1213.87499928
GCTTAGG14900.013.6577181
TAACGCC2457.2759576E-1213.5918384
CTTATAC12100.013.45454537
TTCCTCG11550.013.29437319
TCTCGCC11750.013.22553312
TAGACTA2102.044544E-913.2142875
GCGTTAT2102.044544E-913.2142871
CTACGTT1409.569565E-613.2142864
ATTACAC3100.013.1290323
TAAGACG850.00940907813.0588244
Produced by FastQC (version 0.11.5)