FastQCFastQC Report
Fri 10 Feb 2017
ERR1630214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529460
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48520.19181959785883154No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT45470.17976168826547959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43150.17058977014856924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37480.14817391854387893No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA36260.14335075470653816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.023.1993561
TCTAGAC9500.022.0052623
GTCTAGA7400.022.01
GTTCTAG11000.018.6681821
CTAGAAC9500.018.53
ACTGATC8300.018.58
TAGACCG609.240997E-418.55
TAGAACT9650.018.2124354
AACACGC2750.018.16363736
CTCTAGT10250.018.0487827
CTAGACA11650.017.9442064
TAGACAT11450.017.9344985
ACATGTA10550.017.8862558
TCTAGTT10450.017.88038328
GCAATTA8050.017.46583717
AACGGAG7550.017.39735225
GACGGAC3300.017.3787887
TATACTG3750.017.2666665
CAACGGA7800.017.07692324
TTCTAGA12550.016.952192
Produced by FastQC (version 0.11.5)