FastQCFastQC Report
Fri 10 Feb 2017
ERR1630213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466951
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT39140.15865738719577324No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA34770.14094321289721604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA7700.020.1818181
TCTAGAC8050.019.7639753
GCAATTA7150.019.40559417
ACTGATC7950.019.3144658
CTAGAAC8450.019.2662733
TAGAACT8150.019.0674844
GGTATCA14250.018.824561
TCGTTTA1800.018.530
CTAGACA8950.017.983244
CGATTTG1451.546141E-1017.8620728
ACGGAGT7700.017.5389626
ACATGTA7400.017.58
CGAACGA852.725166E-517.41176416
CGATTTT5350.017.28971921
AACACGC5250.017.26666836
GTTCTAG8600.017.2093031
TATACTG2800.017.1785725
TCTAGAA9700.017.164952
CTTATAC7950.016.52201337
CAACGGA8100.016.44444524
Produced by FastQC (version 0.11.5)