FastQCFastQC Report
Fri 10 Feb 2017
ERR1630212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118471
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC96040.4533458329143991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52250.24664014754037228No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT51270.24201416965349062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.2217165115783978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38730.18282053424380132No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT33310.15723604429798663No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG32410.1529876972590137No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG32360.15275167797907074No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG32020.15114674687545876No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT31380.14812570009218914No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG28670.13533345511928177No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT28550.1347670088474187No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG27490.1297634001126284No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT25720.12140831760264832No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.11890651323525317No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT24380.11508300090017753No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG23030.10871048034171815No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC22940.10828564563782085No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT22330.105406210422517No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG22130.10446213330274523No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC21990.10380127931890501No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT21570.10181871736738432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCA25000.018.64814
TTAGACT2000.018.54
CGCGAAC801.6177195E-518.528
CTAGTAC801.6177195E-518.53
GGTATCA20750.018.4554211
GCATCAG26300.017.72623615
CAGGACA28200.017.7127674
CCAGGAC28300.017.6501773
GCTGCAT26400.017.58901612
GACAGGC26800.017.533587
ATCAAGC26150.017.33269730
TCCAGGA29650.016.9713332
GGACAGG28950.016.742666
CTGCATC27950.016.67978713
ACAGGCT28550.016.5884428
ATTAGAC1901.8189894E-1216.5526313
GGTCTAT904.4488057E-516.4444451
GGCCATC27550.016.38475426
AGGCCAT28250.015.97876125
AGAGGCC29800.015.64429523
Produced by FastQC (version 0.11.5)