FastQCFastQC Report
Fri 10 Feb 2017
ERR1630211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466861
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89791.9232705237747425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62661.3421553738693102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53931.1551618147585685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.5252098590372724No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12880.27588511355628337No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9040.19363365112956532No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.17628373327392952No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7870.16857265867142468No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.12680433790785695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.11695129813798968No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.10666986533464994No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4690.10045816634929883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAG200.00184081737.02
GTACAGA200.00184081737.03
ATTAGAG352.3852934E-531.7142853
GGTAAAC352.3852934E-531.71428535
GTAAACA303.5962337E-430.83333236
GATGTGC250.005493041629.68
ACACCGT250.005493041629.610
CAACCGT250.005493041629.69
ACCGTTT250.005493041629.612
ATCGGTT250.005493041629.625
GTAAGAT250.005493041629.63
GCACTAA250.005493041629.630
GCACCGC405.932692E-527.7510
CACTAAG405.932692E-527.7516
GTATCGA405.932692E-527.7511
CGGTGAT604.3111868E-827.74999814
CAGTCGG604.3111868E-827.74999810
CACATGT358.861476E-426.4285728
ACATGTT358.861476E-426.4285729
AGCTAGT358.861476E-426.428576
Produced by FastQC (version 0.11.5)