##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466861
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729347707347582	31.0	31.0	34.0	30.0	34.0
2	31.920074711745038	33.0	31.0	34.0	30.0	34.0
3	32.00231546434592	33.0	31.0	34.0	30.0	34.0
4	35.68799492782648	37.0	35.0	37.0	33.0	37.0
5	35.643913284682164	37.0	35.0	37.0	33.0	37.0
6	35.73685957918952	37.0	35.0	37.0	35.0	37.0
7	35.707844519032435	37.0	35.0	37.0	33.0	37.0
8	35.746166417841714	37.0	35.0	37.0	33.0	37.0
9	37.44059795099612	39.0	37.0	39.0	34.0	39.0
10	37.31866658384401	39.0	37.0	39.0	34.0	39.0
11	37.405636795534434	39.0	37.0	39.0	34.0	39.0
12	37.33372459897057	39.0	37.0	39.0	34.0	39.0
13	37.37551005545548	39.0	37.0	39.0	34.0	39.0
14	38.472915921441285	40.0	38.0	41.0	34.0	41.0
15	38.47062401871221	40.0	38.0	41.0	34.0	41.0
16	38.3965098819563	40.0	38.0	41.0	34.0	41.0
17	38.453586827771005	40.0	38.0	41.0	34.0	41.0
18	38.472101974677685	40.0	38.0	41.0	34.0	41.0
19	38.54034498490986	40.0	38.0	41.0	34.0	41.0
20	38.534683770972514	40.0	38.0	41.0	34.0	41.0
21	38.499279228721186	40.0	38.0	41.0	34.0	41.0
22	38.46376973017665	40.0	38.0	41.0	34.0	41.0
23	38.40494065685504	40.0	38.0	41.0	34.0	41.0
24	38.37588489935977	40.0	38.0	41.0	34.0	41.0
25	38.34940378399566	40.0	38.0	41.0	34.0	41.0
26	38.13788258175346	40.0	37.0	41.0	33.0	41.0
27	37.97785208016947	40.0	37.0	41.0	33.0	41.0
28	37.9109156686894	40.0	37.0	41.0	33.0	41.0
29	37.803127697537384	40.0	37.0	41.0	33.0	41.0
30	37.635499645504765	40.0	37.0	41.0	33.0	41.0
31	37.45931872655887	39.0	36.0	41.0	33.0	41.0
32	37.31248915630134	39.0	35.0	41.0	32.0	41.0
33	37.174454495021	39.0	35.0	41.0	32.0	41.0
34	37.097405865985806	39.0	35.0	41.0	32.0	41.0
35	36.90506167788699	39.0	35.0	41.0	31.0	41.0
36	36.746228534831566	39.0	35.0	41.0	31.0	41.0
37	36.61750928006409	39.0	35.0	41.0	31.0	41.0
38	36.448441827439005	39.0	35.0	41.0	30.0	41.0
39	36.289094612743405	39.0	35.0	40.0	30.0	41.0
40	36.08952129220474	39.0	35.0	40.0	30.0	41.0
41	35.85909082146506	38.0	35.0	40.0	28.0	41.0
42	35.676102737217285	38.0	35.0	40.0	27.0	41.0
43	34.50709097568655	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	2.0
13	3.0
14	0.0
15	3.0
16	2.0
17	5.0
18	9.0
19	23.0
20	68.0
21	133.0
22	254.0
23	493.0
24	854.0
25	1422.0
26	2195.0
27	3256.0
28	4603.0
29	6189.0
30	8192.0
31	10817.0
32	13778.0
33	17987.0
34	23476.0
35	30841.0
36	43225.0
37	69859.0
38	105418.0
39	123748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.061294475229246	20.227433861470544	13.516014402573786	22.195257260726425
2	18.785462910802146	23.76938746222109	33.65155795836448	23.793591668612287
3	19.361008951272435	26.775421378097548	30.822878758345635	23.040690912284383
4	13.399705693986006	17.051756304338976	36.54363932733726	33.00489867433776
5	12.316299712334079	40.28136854438473	34.11229466586415	13.290037077417047
6	29.82472299035473	37.88750827334046	16.94058831215287	15.347180424151944
7	26.508318321727455	32.45077228554109	22.74210096795406	18.298808424777395
8	29.05318713707078	32.395295387706405	19.832669681125644	18.718847794097172
9	27.299131861517665	13.211855348808319	21.10885252784019	38.38016026183382
10	18.746479144756147	28.345910238807697	31.426270345991636	21.48134027044452
11	32.86759870710982	25.03443209006535	20.598850621491195	21.499118581333633
12	20.41528420664823	30.238550660689157	28.00983590404853	21.336329228614083
13	31.08612627741448	20.540589168938936	25.197435639301634	23.175848914344954
14	22.790723577253186	22.144064293226464	27.63949012661156	27.42572200290879
15	25.659671722418448	28.402458119226065	23.900689926980405	22.03718023137508
16	22.273224792818418	26.384941128087373	26.87566534793011	24.466168731164096
17	20.82097240934668	28.04389315020959	27.871679150753653	23.26345528969008
18	20.156106421397375	25.97839613932198	29.665146585386225	24.200350853894413
19	21.22409025384429	26.232219011654433	32.01038424713138	20.533306487369902
20	21.760009938718376	26.390724434039253	31.43548079621129	20.41378483103108
21	21.907805535266387	26.37551648135098	30.144946782875415	21.57173120050722
22	22.919027290778196	26.099202974761226	29.868204883252186	21.113564851208388
23	21.57622932735868	27.040382469300283	30.114316680982135	21.269071522358903
24	20.32510747310227	27.82819725785619	29.50107205356626	22.345623215475268
25	21.905020980548816	26.970554404844265	30.530072119967183	20.594352494639732
26	21.207382925538866	27.569662062155547	30.053913263262512	21.16904174904308
27	20.281411383688077	28.037895647740978	30.098251942226916	21.58244102634403
28	20.720300046480645	27.928441227688754	31.269478495740703	20.081780230089898
29	21.073510102578712	27.756869817783027	30.737414348167867	20.432205731470397
30	20.624125810466072	29.45073587213325	29.788095385992836	20.13704293140785
31	21.619497023739402	28.344196666673803	30.02970905687132	20.006597252715476
32	20.08156603357316	28.7554539788074	30.056269424946613	21.106710562672827
33	19.670951310989782	28.834920886516542	30.527501761766352	20.966626040727327
34	20.590068564305007	27.560237415419152	30.473095846515342	21.3765981737605
35	20.688170568970207	28.590094267887018	30.52193265233121	20.19980251081157
36	20.339887032757073	29.431458185626987	28.849057856621137	21.379596924994807
37	20.62755295473385	28.208610271579765	30.030137449904792	21.133699323781595
38	20.138328110508265	29.13907994028201	29.89005292795929	20.832539021250437
39	19.31624187927456	28.765521215093997	30.87342913629539	21.044807769336053
40	19.909780427150693	28.15848828666348	31.348302813899643	20.583428472286183
41	19.574562878458472	27.783430185858315	31.048213493952158	21.59379344173105
42	18.751191468124347	28.511698342761548	31.03407652384757	21.703033665266535
43	19.546931527799494	27.298917665000932	31.574280139056377	21.579870668143194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	141.5
2	117.0
3	351.0
4	585.0
5	585.0
6	880.0
7	1175.0
8	1108.5
9	1042.0
10	1452.0
11	1862.0
12	1862.0
13	4025.0
14	6188.0
15	11130.0
16	16072.0
17	13154.0
18	10236.0
19	10236.0
20	10965.5
21	11695.0
22	8041.5
23	4388.0
24	4684.0
25	4980.0
26	4980.0
27	5582.0
28	6184.0
29	7530.5
30	8877.0
31	10321.5
32	11766.0
33	11766.0
34	14520.0
35	17274.0
36	19510.5
37	21747.0
38	23701.5
39	25656.0
40	25656.0
41	29213.5
42	32771.0
43	35245.5
44	37720.0
45	39749.5
46	41779.0
47	41779.0
48	42895.0
49	44011.0
50	41056.0
51	38101.0
52	35064.5
53	32028.0
54	32028.0
55	30049.5
56	28071.0
57	24385.5
58	20700.0
59	18265.5
60	15831.0
61	15831.0
62	13541.0
63	11251.0
64	8596.0
65	5941.0
66	4749.0
67	3557.0
68	3557.0
69	2695.0
70	1833.0
71	1419.5
72	1006.0
73	880.5
74	755.0
75	755.0
76	610.0
77	465.0
78	423.0
79	381.0
80	320.0
81	259.0
82	259.0
83	222.5
84	186.0
85	143.0
86	100.0
87	86.0
88	72.0
89	72.0
90	49.0
91	26.0
92	15.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.170469269725512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28110366887243	19.798516711463638
2	11.26689983340663	6.3478771104411384
3	4.670145298606105	3.94680553858609
4	2.6330199577144846	2.966936312214794
5	1.7430258351651278	2.4550927862928438
6	1.2770223212772116	2.158459083497594
7	0.9309725803520047	1.8358154126034247
8	0.7334832918390385	1.6530054826086997
9	0.5856763842510906	1.4848900726094367
>10	5.015779674457783	28.648314710113482
>50	0.6069799733312542	11.722944513275634
>100	0.24827537177664855	10.967760503050345
>500	0.0038079044751019716	0.7835034106087371
>1k	0.0015231617900407885	0.8023830108643693
>5k	0.0022847426850611825	4.427695341769747
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8979	1.9232705237747425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6266	1.3421553738693102	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5393	1.1551618147585685	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2452	0.5252098590372724	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1288	0.27588511355628337	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	904	0.19363365112956532	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.17628373327392952	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	787	0.16857265867142468	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	592	0.12680433790785695	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.11695129813798968	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	498	0.10666986533464994	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	469	0.10045816634929883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.1419651673624482E-4	0.0
10	0.0	0.0	0.0	2.1419651673624482E-4	0.0
11	0.0	0.0	0.0	2.1419651673624482E-4	0.0
12	0.0	0.0	0.0	2.1419651673624482E-4	0.0
13	0.0	0.0	0.0	2.1419651673624482E-4	0.0
14	0.0	0.0	0.0	2.1419651673624482E-4	0.0
15	0.0	0.0	0.0	2.1419651673624482E-4	0.0
16	0.0	0.0	0.0	8.567860669449793E-4	0.0
17	0.0	0.0	0.0	0.0017135721338899586	0.0
18	0.0	0.0	0.0	0.0019277686506262036	0.0
19	0.0	0.0	0.0	0.0023561616840986933	0.0
20	0.0	0.0	0.0	0.0029987512343074276	0.0
21	0.0	0.0	0.0	0.0036413407845161624	0.0
22	0.0	0.0	0.0	0.0062116989853511	0.0
23	0.0	0.0	0.0	0.009210450219658528	0.0
24	0.0	0.0	0.0	0.013922773587855914	0.0
25	0.0	0.0	0.0	0.01692152482216334	0.0
26	0.0	0.0	0.0	0.02120545515688824	0.0
27	0.0	0.0	0.0	0.030415905376546766	0.0
28	0.0	0.0	0.0	0.05312073615058872	0.0
29	0.0	0.0	0.0	0.08953414399575034	0.0
30	0.0	0.0	0.0	0.15871961890155742	0.0
31	0.0	0.0	0.0	0.3564230038491114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAG	20	0.001840817	37.0	2
GTACAGA	20	0.001840817	37.0	3
ATTAGAG	35	2.3852934E-5	31.714285	3
GGTAAAC	35	2.3852934E-5	31.714285	35
GTAAACA	30	3.5962337E-4	30.833332	36
GATGTGC	25	0.0054930416	29.6	8
ACACCGT	25	0.0054930416	29.6	10
CAACCGT	25	0.0054930416	29.6	9
ACCGTTT	25	0.0054930416	29.6	12
ATCGGTT	25	0.0054930416	29.6	25
GTAAGAT	25	0.0054930416	29.6	3
GCACTAA	25	0.0054930416	29.6	30
GCACCGC	40	5.932692E-5	27.75	10
CACTAAG	40	5.932692E-5	27.75	16
GTATCGA	40	5.932692E-5	27.75	11
CGGTGAT	60	4.3111868E-8	27.749998	14
CAGTCGG	60	4.3111868E-8	27.749998	10
CACATGT	35	8.861476E-4	26.42857	28
ACATGTT	35	8.861476E-4	26.42857	29
AGCTAGT	35	8.861476E-4	26.42857	6
>>END_MODULE