FastQCFastQC Report
Fri 10 Feb 2017
ERR1630210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738792
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.24839083685685234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33030.1899594661121054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30220.17379882125061538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA400.001931583523.12531
AAGACGG4100.019.853665
GACGGAC4250.018.7176467
CGGACCA4450.017.8764069
AGACGGA4700.017.7127676
TAACGGC852.7244163E-517.41176436
TTAACGG852.7244163E-517.41176435
CTAATCG752.0680351E-417.26666626
ACGGACC4650.017.1075278
GGTATCA14650.016.9215011
TACGACG3200.016.7656255
CGCAAGA5150.016.1650492
CTTATAC7850.016.02547837
ATAGACC700.002593211415.8571423
TGGCGTA1302.5909867E-715.65384624
ACGGTAT3450.015.5507259
CAAGACG5850.015.4957274
GTCTAAT1802.0190782E-1015.4166671
TCTATAC2400.015.4166673
GTAAACG3250.015.3692327
Produced by FastQC (version 0.11.5)