FastQCFastQC Report
Fri 10 Feb 2017
ERR1630209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983034
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17580.17883409932921954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.16021826305092193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11780.11983308817395939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG1200.023.1250025
TCGCGGA652.6821908E-622.7692332
TTAACGG502.7017167E-422.235
CGTTATA450.003825420520.55555513
GACGGAC1101.7535058E-920.1818187
TCCGTAT555.142365E-420.18181813
CGAACTA656.900833E-519.92307729
CGCGGAA759.263533E-619.73333433
GTTCGCG759.263533E-619.73333430
GGTATCA7850.019.324841
AACCGTT500.00703417518.510
GAGCGAA1306.9667294E-1018.516
CGAAAGC1401.8681021E-917.17857219
GAGCCGT650.001579714417.0769236
CGCGCTT1107.804774E-716.81818212
CGTCCTA1005.876049E-616.65000233
GCGAAAG1352.2178938E-816.44444518
CAATACC1251.656881E-716.2799994
AGACGGA1504.667527E-916.0333336
AGCGAAA1504.667527E-916.03333317
Produced by FastQC (version 0.11.5)