FastQCFastQC Report
Fri 10 Feb 2017
ERR1630208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159324
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52253.2794808064070704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35492.2275363410408975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29901.8766789686425145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.9471266099269413No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8110.5090256333006954No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6330.397303607742713No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.2981346187642791No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.2862092340137079No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4400.27616680475006905No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.23913534684040072No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.19771032612789036No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.1970826742989129No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3080.19331676332504832No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3030.1901785041801612No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2950.18515728954834174No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2730.1713489493108383No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2560.16067886821822197No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.16067886821822197No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2490.15628530541538No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2470.15503000175742512No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2450.15377469809947025No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2400.15063643895458312No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2360.1481258316386734No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2350.14749817980969596No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2300.14435992066480882No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2180.13682809871707965No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2160.1355727950591248No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2110.13243453591423765No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA2060.12929627676935052No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1990.12490271396650851No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1990.12490271396650851No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA1990.12490271396650851No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1930.12113680299264391No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1830.11486028470286962No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1790.11234967738695992No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1780.11172202555798248No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1770.11109437372900505No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1730.10858376641309532No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1670.10481785543923075No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1650.1035625517812759No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.10230724812332102No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.10167959629434359No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1610.10105194446536617No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1610.10105194446536617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT251.2288577E-437.036
AAATCAG251.2288577E-437.033
AATCAGG251.2288577E-437.034
ATGCCCC200.001837180737.037
TCGTGGT303.585698E-430.83333235
GTGGTTT303.585698E-430.83333237
TCAGGCA250.005482317429.636
TACTACT250.005482317429.62
TGGTACT250.005482317429.627
TGATCGT358.835758E-426.42857232
ACGGGCG358.835758E-426.4285725
ATCGTGG358.835758E-426.42857234
AGACCGT358.835758E-426.42857221
CGGCTGA358.835758E-426.42857228
CATCCAG358.835758E-426.42857216
CGCTTGG358.835758E-426.42857216
AGTGGCT551.889355E-523.54545427
GACCGTC400.001923932523.12500222
ATACGGG400.001923932523.1250023
TCTTATA4250.021.76470637
Produced by FastQC (version 0.11.5)