FastQCFastQC Report
Fri 10 Feb 2017
ERR1630207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095283
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53350.48708872501444833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40790.37241516576081246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32880.30019638760028233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.17201033888045372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACAC705.1015595E-621.1428593
GCGTCAA450.003825694320.5555579
TCACGTA701.2191305E-418.525
CTACGCA500.007034676618.49999827
GCGTATA500.007034676618.4999981
TCTAGCG852.722927E-517.41176428
GTACTAA650.001579870217.0769211
CGCAATA803.382384E-416.187536
CGGTGAT1302.58844E-715.65384514
TAGTACT1302.58844E-715.6538454
TAGACAG1902.7284841E-1115.5789485
CGCGCAA957.0591894E-515.57894821
TTAAGAC957.0591894E-515.5789483
CTAGCGG957.0591894E-515.57894829
CGGTCCA1201.9351992E-615.41666610
TCTTGCG1455.343827E-815.3103452
CTCTATG3050.015.1639351
TTCTATA1101.4516647E-515.1363642
GTGTAAG1853.0377123E-1015.01
CGTCAAA750.004104241714.810
Produced by FastQC (version 0.11.5)