FastQCFastQC Report
Fri 10 Feb 2017
ERR1630206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106457
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32503.052875809012089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31832.9899396000263017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19561.8373615638238912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13701.2869045717989422No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7610.7148426125102154No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.5344881031778089No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4640.43585673088664906No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.4255239204561466No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.4104943780117794No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.3550729402481753No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.28180392083188516No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2820.2648956855819721No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2690.2526841823459237No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2340.21980705824887042No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA2190.20571686220727617No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.19820209098509256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.18880862695736308No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1870.17565777731854176No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.15499215645753686No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.14184130681871554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1480.13902326761039668No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.133387189193759No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1370.12869045717989425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1340.12587241797157536No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA1330.12493307156880243No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1290.12117568595771064No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1220.11460026113829996No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA1220.11460026113829996No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA1210.11366091473552702No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA1180.11084287552720817No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA1140.10708548991611637No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.10332810430502457No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAA1090.10238875790225162No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTT1090.10238875790225162No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1080.10144941149947866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT200.001834444437.037
CTCCGAG200.001834444437.012
TCCGAGC200.001834444437.013
AGCCCAC200.001834444437.017
CACGAGA200.001834444437.021
GCGCAGA200.001834444437.031
CCCACGA200.001834444437.019
ACATGCG200.001834444437.027
TCTCCGA200.001834444437.011
ATGCGCA200.001834444437.029
GCTGTGG200.001834444437.026
ACGAGAC200.001834444437.022
AGCTGTA303.5777723E-430.8333327
AGCCGTC250.005474244729.59999812
AGTCCTA250.005474244729.59999829
TCCATGA250.005474244729.5999982
ATCTCCG250.005474244729.59999810
GCCCACG250.005474244729.59999818
CGAGTCC250.005474244729.59999827
CGACGAG250.005474244729.59999824
Produced by FastQC (version 0.11.5)