##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	106457
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70030153019529	31.0	31.0	34.0	30.0	34.0
2	31.794827958706332	31.0	31.0	34.0	30.0	34.0
3	31.77849272476211	33.0	31.0	34.0	30.0	34.0
4	35.544689405111924	37.0	35.0	37.0	33.0	37.0
5	35.52314079863231	37.0	35.0	37.0	33.0	37.0
6	35.65304301267178	37.0	35.0	37.0	33.0	37.0
7	35.61374075917976	37.0	35.0	37.0	33.0	37.0
8	35.69273979165297	37.0	35.0	37.0	33.0	37.0
9	37.30163352339442	39.0	37.0	39.0	34.0	39.0
10	37.19490498511136	39.0	37.0	39.0	34.0	39.0
11	37.3036249377683	39.0	37.0	39.0	34.0	39.0
12	37.23431996017171	39.0	37.0	39.0	34.0	39.0
13	37.304573677635105	39.0	37.0	39.0	34.0	39.0
14	38.28727091689603	40.0	38.0	41.0	34.0	41.0
15	38.266501967930715	40.0	38.0	41.0	33.0	41.0
16	38.08980151610509	40.0	37.0	41.0	33.0	41.0
17	38.290398940417255	40.0	38.0	41.0	34.0	41.0
18	38.40747907605888	40.0	38.0	41.0	34.0	41.0
19	38.469006265440505	40.0	38.0	41.0	34.0	41.0
20	38.50508656077101	40.0	38.0	41.0	34.0	41.0
21	38.500333467972986	40.0	38.0	41.0	34.0	41.0
22	38.433142019782636	40.0	38.0	41.0	34.0	41.0
23	38.359797852654125	40.0	38.0	41.0	34.0	41.0
24	38.29859943451346	40.0	38.0	41.0	34.0	41.0
25	38.26347727251378	40.0	37.0	41.0	34.0	41.0
26	37.97145326281973	40.0	37.0	41.0	33.0	41.0
27	37.77684886855726	40.0	36.0	41.0	33.0	41.0
28	37.57345219196483	39.0	36.0	41.0	33.0	41.0
29	37.36838347877547	39.0	35.0	41.0	33.0	41.0
30	37.125102153921304	39.0	35.0	41.0	33.0	41.0
31	36.76736146988925	38.0	35.0	40.0	32.0	41.0
32	36.49196389152428	38.0	35.0	40.0	31.0	41.0
33	36.257972702593534	38.0	35.0	40.0	31.0	41.0
34	36.03535699860037	38.0	35.0	40.0	30.0	41.0
35	35.84838009712842	38.0	35.0	40.0	30.0	41.0
36	35.60222437228177	38.0	35.0	40.0	30.0	41.0
37	35.331147787369545	38.0	35.0	40.0	27.0	41.0
38	34.99036230590755	38.0	35.0	40.0	25.0	41.0
39	34.6383798153245	37.0	34.0	40.0	24.0	41.0
40	34.23857519937627	37.0	33.0	40.0	21.0	41.0
41	33.77217092347145	37.0	33.0	40.0	18.0	41.0
42	33.339573724602424	37.0	33.0	40.0	15.0	41.0
43	32.242332584987366	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	7.0
19	11.0
20	19.0
21	44.0
22	87.0
23	200.0
24	303.0
25	495.0
26	784.0
27	1135.0
28	1385.0
29	1988.0
30	2392.0
31	3013.0
32	3807.0
33	4950.0
34	6242.0
35	7996.0
36	11250.0
37	17947.0
38	21256.0
39	21142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.847487718045784	19.69433668053768	14.464055909897894	17.99411969151864
2	18.6554195590708	27.259832608471022	32.02325821693266	22.061489615525517
3	21.759020073832627	27.97937195299511	32.84612566576176	17.415482307410503
4	14.728951595479863	18.617845702959883	32.29660802013959	34.35659468142067
5	12.32986088279775	41.21288407526043	31.570493250796094	14.88676179114572
6	28.90274946692092	33.753534290840435	17.753647012408766	19.590069229829883
7	22.939778502118227	36.57908827038147	22.311355758663122	18.169777468837182
8	30.616117305578776	32.05143860901585	19.82772386973144	17.504720215673935
9	27.993462149036702	12.426613562283363	22.558403862592407	37.02152042608753
10	20.891063997670418	30.561635214217947	27.064448556694252	21.48285223141738
11	28.77687704894934	26.94703025634763	18.74841485294532	25.527677841757708
12	19.030218773777204	34.14148435518566	28.104305024563907	18.723991846473222
13	31.95562527593301	20.55102059986661	27.319011431845723	20.17434269235466
14	22.82236020177161	25.02324882346863	29.675831556403054	22.47855941835671
15	28.02164254111989	26.784523328667913	26.269761499948334	18.924072630263865
16	18.8151084475422	27.361282019970506	31.10363808861794	22.719971443869355
17	18.020421390796283	31.09424462459021	29.8721549545826	21.013179030030905
18	16.706275773316925	25.65918633814592	33.684022657035236	23.95051523150192
19	17.772433940464225	29.09813351869769	36.61948016570071	16.50995237513738
20	19.361808053956057	26.11195130428248	37.31929323576655	17.20694740599491
21	21.478155499403513	26.405027381947644	34.80090552993227	17.31591158871657
22	21.687629747221884	27.032510778999974	33.85780174154823	17.422057732229916
23	19.899114196342186	27.891073391134448	33.83995415989555	18.36985825262782
24	17.739556816367173	29.99333064054031	34.55949350441963	17.70761903867289
25	18.049541129282247	29.29539626328001	34.45428670730906	18.20077590012869
26	19.364626093164375	28.36826136374311	34.12175808072743	18.145354462365088
27	18.15568727279559	29.335788158599247	34.194087753740945	18.31443681486422
28	17.535718646965442	30.423551293010327	35.16725062701372	16.87347943301051
29	17.942455639366127	29.788553124735813	35.125919385291716	17.143071850606347
30	16.758879171872213	31.926505537447042	35.269639384915976	16.04497590576477
31	17.71043707788121	30.789896390091776	34.49749664183661	17.002169890190405
32	16.14360727805593	31.857933250044617	34.475891674572836	17.522567797326623
33	16.24975342156927	32.053317301821394	35.25554918887438	16.441380087734956
34	15.817654076293714	32.480719915083085	33.89443625125638	17.807189757366825
35	15.966070807931839	32.4074508956668	34.06070056454718	17.565777731854176
36	15.539607541072922	32.84518631935899	33.000178475816526	18.61502766375156
37	15.764111331335656	33.06029664559399	33.269770893412364	17.905821129657983
38	16.02618897770931	32.198915994251195	33.279164357440095	18.495730670599396
39	16.149243356472567	31.481255342532666	34.98219938566745	17.387301915327317
40	15.750960481696834	31.758362531350688	34.592370628516676	17.8983063584358
41	15.140385319894417	31.816602008322608	34.93805010473712	18.10496256704585
42	14.610593948730473	32.37833115718083	34.4458325896841	18.565242304404595
43	14.870792902298579	32.388663967611336	34.210056642588086	18.530486487501996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	35.0
2	61.0
3	243.5
4	426.0
5	426.0
6	603.5
7	781.0
8	782.0
9	783.0
10	1020.0
11	1257.0
12	1257.0
13	2504.5
14	3752.0
15	5589.0
16	7426.0
17	5977.5
18	4529.0
19	4529.0
20	5210.0
21	5891.0
22	3919.0
23	1947.0
24	2012.0
25	2077.0
26	2077.0
27	2149.0
28	2221.0
29	2221.0
30	2221.0
31	2388.5
32	2556.0
33	2556.0
34	2806.5
35	3057.0
36	3227.0
37	3397.0
38	3772.0
39	4147.0
40	4147.0
41	4701.5
42	5256.0
43	5836.5
44	6417.0
45	7146.5
46	7876.0
47	7876.0
48	9094.5
49	10313.0
50	8786.5
51	7260.0
52	6402.0
53	5544.0
54	5544.0
55	5075.0
56	4606.0
57	4396.5
58	4187.0
59	3630.0
60	3073.0
61	3073.0
62	2576.5
63	2080.0
64	1674.5
65	1269.0
66	1084.5
67	900.0
68	900.0
69	690.0
70	480.0
71	386.0
72	292.0
73	244.0
74	196.0
75	196.0
76	145.5
77	95.0
78	67.0
79	39.0
80	28.0
81	17.0
82	17.0
83	14.0
84	11.0
85	8.5
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	106457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.94999859098039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46195586639335	28.61343077486685
2	11.402387555769925	8.88245958462102
3	4.886048474617147	5.709347436053994
4	2.549137827083082	3.971556590924035
5	1.6905824189075123	3.2924091417191916
6	1.0731942602194622	2.5080548954037782
7	0.750030145906186	2.0449571188367135
8	0.5763897262751718	1.7960303221018814
9	0.39310261666465696	1.3780211728679186
>10	2.9663571686964914	21.485670270625697
>50	0.16399372965151332	4.348234498435988
>100	0.07235017484625587	5.553415933193683
>500	0.004823344989750392	1.2493307156880245
>1k	0.009646689979500784	9.167081544661224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3250	3.052875809012089	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3183	2.9899396000263017	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1956	1.8373615638238912	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1370	1.2869045717989422	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	761	0.7148426125102154	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	569	0.5344881031778089	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	464	0.43585673088664906	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	453	0.4255239204561466	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	437	0.4104943780117794	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.3550729402481753	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	300	0.28180392083188516	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	282	0.2648956855819721	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	269	0.2526841823459237	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	234	0.21980705824887042	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	219	0.20571686220727617	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	211	0.19820209098509256	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.18880862695736308	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	187	0.17565777731854176	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.15499215645753686	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151	0.14184130681871554	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	148	0.13902326761039668	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142	0.133387189193759	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	137	0.12869045717989425	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	134	0.12587241797157536	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	133	0.12493307156880243	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	129	0.12117568595771064	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	122	0.11460026113829996	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	122	0.11460026113829996	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	121	0.11366091473552702	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	118	0.11084287552720817	No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA	114	0.10708548991611637	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110	0.10332810430502457	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAA	109	0.10238875790225162	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTT	109	0.10238875790225162	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	108	0.10144941149947866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0037573856110918024	0.0
17	0.0	0.0	0.0	0.009393464027729505	0.0
18	0.0	0.0	0.0	0.011272156833275407	0.0
19	0.0	0.0	0.0	0.01502954244436721	0.0
20	0.0	0.0	0.0	0.01690823524991311	0.0
21	0.0	0.0	0.0	0.022544313666550814	0.0
22	0.0	0.0	0.0	0.038513202513690975	0.0
23	0.0	0.0	0.0	0.05542143776360409	0.0
24	0.0	0.0	0.0	0.09581333308284096	0.0
25	0.0	0.0	0.0	0.09957071869393276	0.0
26	0.0	0.0	0.0	0.11084287552720817	0.0
27	0.0	0.0	0.0	0.1352658819993049	0.0
28	0.0	0.0	0.0	0.16250692767972044	0.0
29	0.0	0.0	0.0	0.22544313666550814	0.0
30	0.0	0.0	0.0	0.41331241722009826	0.0
31	0.0	0.0	0.0	0.778718167898776	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	20	0.0018344444	37.0	37
CTCCGAG	20	0.0018344444	37.0	12
TCCGAGC	20	0.0018344444	37.0	13
AGCCCAC	20	0.0018344444	37.0	17
CACGAGA	20	0.0018344444	37.0	21
GCGCAGA	20	0.0018344444	37.0	31
CCCACGA	20	0.0018344444	37.0	19
ACATGCG	20	0.0018344444	37.0	27
TCTCCGA	20	0.0018344444	37.0	11
ATGCGCA	20	0.0018344444	37.0	29
GCTGTGG	20	0.0018344444	37.0	26
ACGAGAC	20	0.0018344444	37.0	22
AGCTGTA	30	3.5777723E-4	30.833332	7
AGCCGTC	25	0.0054742447	29.599998	12
AGTCCTA	25	0.0054742447	29.599998	29
TCCATGA	25	0.0054742447	29.599998	2
ATCTCCG	25	0.0054742447	29.599998	10
GCCCACG	25	0.0054742447	29.599998	18
CGAGTCC	25	0.0054742447	29.599998	27
CGACGAG	25	0.0054742447	29.599998	24
>>END_MODULE