FastQCFastQC Report
Fri 10 Feb 2017
ERR1630205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338566
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG34490.1474835433338208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33260.14222390986613165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.1419245811321981No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26260.11229103647277862No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT25730.11002469034442475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG806.9664566E-720.81255
GGTATCA14650.020.4573381
TCTAGCG2900.019.13793228
CTAGCGG3000.018.49999829
CGCAATA3200.017.92187536
GTAAACG3100.016.11290227
ATCTCGC6750.015.89629611
ATATCGT700.002593568815.8571436
CTTATAC9400.015.74468137
GCTTAGG10200.015.5980391
TAGGACA11050.015.5701364
TAGTACT2055.456968E-1215.3414644
ACGGACC4000.015.2625018
GTACTAG855.367098E-415.2352941
CGGCCTT7050.015.21985724
CTTAGGA10950.015.036532
TTAGGAC11400.014.9298243
AAGACGG4250.014.85
TATACTG3000.014.7999995
TCTCGCC7650.014.75163412
Produced by FastQC (version 0.11.5)