FastQCFastQC Report
Fri 10 Feb 2017
ERR1630204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566980
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC76600.2984051297633795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71110.2770181302542287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.22310263422387397No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT46640.18169210511963474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43480.16938191960981386No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT30000.1168688497767805No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG29250.11394712853236097No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG29190.11371339083280742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.10463657683347748No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT26620.10370162603526323No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT26570.1035068446189686No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG26450.10303936921986147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG500.007037188418.55
CAGGACA23950.017.8434244
CGCGAAC852.7252341E-517.41176428
TGCATCA20650.017.38014614
GACAGGC21950.017.3621867
CCAGGAC23900.017.3389133
GCATCAG21250.016.9764715
GGTATCA30450.016.9507391
CTGCATC22950.015.96078413
GCTGCAT23000.015.92608612
ACAGGCT23650.015.801278
GTATAGA3000.015.4166671
TCCAGGA26200.015.393132
ATCAAGC23100.015.37662330
CGCAAGA4250.014.82
TCTAGCG3150.014.6825428
CTAGCGG3150.014.6825429
AGAGGCC24550.014.61914423
GGACAGG26900.014.5799266
ACTGCGT1152.2118342E-514.4782618
Produced by FastQC (version 0.11.5)