##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630204.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2566980
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879412772986154	33.0	31.0	34.0	30.0	34.0
2	32.072736834724076	33.0	31.0	34.0	30.0	34.0
3	32.20575773866567	34.0	31.0	34.0	30.0	34.0
4	35.849195942313536	37.0	35.0	37.0	35.0	37.0
5	35.77025921510881	37.0	35.0	37.0	35.0	37.0
6	35.8292651286726	37.0	35.0	37.0	35.0	37.0
7	35.80557892932551	37.0	35.0	37.0	35.0	37.0
8	35.78886317774194	37.0	35.0	37.0	35.0	37.0
9	37.5034893142915	39.0	37.0	39.0	35.0	39.0
10	37.42209912036712	39.0	37.0	39.0	34.0	39.0
11	37.49743005399341	39.0	37.0	39.0	35.0	39.0
12	37.38509181995964	39.0	37.0	39.0	34.0	39.0
13	37.41099774832683	39.0	37.0	39.0	34.0	39.0
14	38.64700348269172	40.0	38.0	41.0	34.0	41.0
15	38.64164037117547	40.0	38.0	41.0	34.0	41.0
16	38.62571387389072	40.0	38.0	41.0	34.0	41.0
17	38.590774372998624	40.0	38.0	41.0	34.0	41.0
18	38.523709573117046	40.0	38.0	41.0	34.0	41.0
19	38.54121185205962	40.0	38.0	41.0	34.0	41.0
20	38.49455897591722	40.0	38.0	41.0	34.0	41.0
21	38.46141652837186	40.0	38.0	41.0	34.0	41.0
22	38.42317431378507	40.0	38.0	41.0	34.0	41.0
23	38.372007183538635	40.0	38.0	41.0	34.0	41.0
24	38.35440361825959	40.0	38.0	41.0	34.0	41.0
25	38.31651395803629	40.0	38.0	41.0	34.0	41.0
26	38.14469766028563	40.0	38.0	41.0	33.0	41.0
27	38.05265292288993	40.0	38.0	41.0	33.0	41.0
28	37.95796733905212	40.0	37.0	41.0	33.0	41.0
29	37.88067339831241	40.0	37.0	41.0	33.0	41.0
30	37.82684321654239	40.0	37.0	41.0	33.0	41.0
31	37.73129708840739	40.0	37.0	41.0	33.0	41.0
32	37.64072528808172	40.0	37.0	41.0	32.0	41.0
33	37.56473092895153	40.0	37.0	41.0	32.0	41.0
34	37.46532696008539	40.0	37.0	41.0	32.0	41.0
35	37.362106054585546	40.0	36.0	41.0	31.0	41.0
36	37.258056159377944	40.0	36.0	41.0	31.0	41.0
37	37.18230722483229	39.0	36.0	41.0	31.0	41.0
38	37.083293987487245	39.0	36.0	41.0	31.0	41.0
39	36.98685770827977	39.0	36.0	41.0	31.0	41.0
40	36.8659522084317	39.0	35.0	41.0	30.0	41.0
41	36.72282136985874	39.0	35.0	41.0	30.0	41.0
42	36.65692370022361	39.0	35.0	41.0	30.0	41.0
43	35.46104722280656	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	5.0
11	4.0
12	5.0
13	4.0
14	5.0
15	7.0
16	22.0
17	50.0
18	117.0
19	237.0
20	486.0
21	879.0
22	1678.0
23	2810.0
24	4434.0
25	6907.0
26	10188.0
27	15144.0
28	21214.0
29	29044.0
30	39407.0
31	51781.0
32	67769.0
33	88340.0
34	119480.0
35	159869.0
36	220112.0
37	331608.0
38	597859.0
39	797512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60305884736149	18.61993470926926	12.105509197578478	26.671497245790775
2	19.52983661734801	19.953408285222324	34.09898012450428	26.417774972925383
3	20.658594924775418	22.105509197578478	28.915924549470585	28.31997132817552
4	15.316402932629003	15.176277181746645	33.21463353824338	36.29268634738097
5	15.548309686869397	35.7850470202339	33.072443104348295	15.59420018854841
6	36.10047604578143	33.40022127168891	15.23525699460066	15.264045687929006
7	30.138100024152898	29.408604663846234	20.752206873446617	19.701088438554255
8	27.729588855386485	32.94201746799742	19.275335218817442	20.05305845779866
9	27.06947463556397	13.82749378647282	19.338833960529495	39.764197617433716
10	18.192895932184904	25.954818502676297	31.981589260531827	23.87069630460697
11	36.51146483416311	21.295023724376506	20.646518476965152	21.546992964495242
12	22.569634356325334	24.63840777879064	28.09211602739406	24.69984183748997
13	30.42594800115311	19.173347669245572	24.303617480463423	26.097086849137895
14	23.26243289780209	20.39634122587632	24.437510226024354	31.903715650297237
15	26.30075029801557	26.80850649401242	21.3526011110332	25.538142096938817
16	25.60000467475399	25.29283438125735	23.127488332593163	25.979672611395493
17	24.18943661423151	25.996151119214016	24.921074570117412	24.893337696437058
18	24.366648746776367	24.181645357579722	25.891631411230314	25.56007448441359
19	24.989325978387054	24.410046046326812	25.67164527966716	24.928982695618977
20	24.870236620464514	24.735253098972336	25.546634566689264	24.84787571387389
21	26.403594885819132	24.24498827415874	24.835760309780362	24.515656530241763
22	25.809706347536792	24.5324077320431	25.126880614574326	24.53100530584578
23	25.012037491527007	24.762171890704252	25.04850057265736	25.177290045111377
24	25.233971437253118	24.87257399746005	25.163966996236827	24.72948756905001
25	25.368526439629445	24.423485964051142	25.275304053790837	24.932683542528576
26	25.50865219051181	25.005726573639066	25.337283500455786	24.14833773539334
27	25.892254711762458	24.420875893072793	24.641524281451353	25.045345113713392
28	24.662210067861846	24.264972847470567	25.989762288759554	25.083054795908033
29	24.556872277929706	24.442730367981053	26.092879570545936	24.907517783543305
30	24.317797567569674	24.87109365869621	25.844572221053536	24.966536552680584
31	24.74561547031921	24.938799679000226	25.437206367014937	24.87837848366563
32	24.243780629377714	24.615034008835284	25.553841479092164	25.587343882694842
33	24.616202697333055	24.80506275857233	25.463073339098862	25.115661204995753
34	25.37417510070199	24.68418141161988	25.343555462060475	24.598088025617653
35	25.474331704960697	24.43193947751833	25.841338849543042	24.252389967977937
36	24.885702264918308	25.10522092108236	24.7364217874701	25.27265502652923
37	24.642965663931935	24.4540666464094	25.64616787041582	25.256799819242847
38	24.836383610312506	24.499178022423237	25.951857825148615	24.71258054211564
39	24.1171727087862	24.634278412765195	26.071141964487452	25.177406913961153
40	24.52005859025002	23.96212670141567	26.78817131415126	24.72964339418305
41	23.484016236978864	24.19847447194758	27.32241778276418	24.995091508309375
42	23.870150916641343	24.655743324840866	26.41029536653967	25.06381039197812
43	23.76076167325028	23.217126740371956	27.358880863894537	25.663230722483227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	274.0
2	274.0
3	644.5
4	1015.0
5	1015.0
6	1332.0
7	1649.0
8	1552.5
9	1456.0
10	2036.0
11	2616.0
12	2616.0
13	4896.0
14	7176.0
15	10366.5
16	13557.0
17	13051.5
18	12546.0
19	12546.0
20	15879.5
21	19213.0
22	18690.5
23	18168.0
24	22489.0
25	26810.0
26	26810.0
27	32178.0
28	37546.0
29	43797.0
30	50048.0
31	58483.5
32	66919.0
33	66919.0
34	77929.5
35	88940.0
36	99588.5
37	110237.0
38	117504.0
39	124771.0
40	124771.0
41	134769.0
42	144767.0
43	148150.5
44	151534.0
45	156918.0
46	162302.0
47	162302.0
48	163958.0
49	165614.0
50	169984.0
51	174354.0
52	180690.0
53	187026.0
54	187026.0
55	187282.5
56	187539.0
57	178129.0
58	168719.0
59	155677.5
60	142636.0
61	142636.0
62	135553.0
63	128470.0
64	112490.0
65	96510.0
66	83003.5
67	69497.0
68	69497.0
69	60341.5
70	51186.0
71	46415.0
72	41644.0
73	42563.0
74	43482.0
75	43482.0
76	39952.0
77	36422.0
78	28065.0
79	19708.0
80	12884.5
81	6061.0
82	6061.0
83	4243.0
84	2425.0
85	2015.5
86	1606.0
87	1383.0
88	1160.0
89	1160.0
90	1009.5
91	859.0
92	512.5
93	166.0
94	115.0
95	64.0
96	64.0
97	37.5
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2566980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.205897944186745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1373503888089	37.3574968602746
2	11.981742857445173	11.312178610440906
3	3.711425628145573	5.256035382890373
4	1.5127917599530012	2.856507737245921
5	0.8240374596953518	1.9449714112282837
6	0.5165747750002097	1.463122566552068
7	0.3434762481640456	1.1349873301958868
8	0.24991220580593718	0.9437864065825332
9	0.20105934148211693	0.8542068079257183
>10	1.2236397764809979	11.265671963665543
>50	0.15740617798290785	5.2364905846433505
>100	0.12222343194411937	11.320635655256456
>500	0.012901585848289952	4.138989917531817
>1k	0.005210255823347688	4.114667720181995
>5k	2.4810742015941373E-4	0.800251045384416
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	7660	0.2984051297633795	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7111	0.2770181302542287	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5727	0.22310263422387397	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4664	0.18169210511963474	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4348	0.16938191960981386	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3000	0.1168688497767805	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2925	0.11394712853236097	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2919	0.11371339083280742	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2686	0.10463657683347748	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2662	0.10370162603526323	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2657	0.1035068446189686	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2645	0.10303936921986147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.895628325892683E-5	0.0	0.0	0.0	3.895628325892683E-5
3	3.895628325892683E-5	0.0	0.0	0.0	3.895628325892683E-5
4	3.895628325892683E-5	0.0	0.0	0.0	3.895628325892683E-5
5	1.5582513303570732E-4	0.0	0.0	0.0	3.895628325892683E-5
6	1.5582513303570732E-4	0.0	0.0	0.0	3.895628325892683E-5
7	1.5582513303570732E-4	0.0	0.0	0.0	3.895628325892683E-5
8	1.5582513303570732E-4	0.0	0.0	0.0	3.895628325892683E-5
9	1.5582513303570732E-4	0.0	0.0	0.0	3.895628325892683E-5
10	1.5582513303570732E-4	0.0	0.0	0.0	3.895628325892683E-5
11	1.9478141629463417E-4	0.0	0.0	7.791256651785366E-5	3.895628325892683E-5
12	1.9478141629463417E-4	0.0	0.0	7.791256651785366E-5	3.895628325892683E-5
13	2.33737699553561E-4	0.0	0.0	1.168688497767805E-4	3.895628325892683E-5
14	2.33737699553561E-4	0.0	0.0	1.5582513303570732E-4	3.895628325892683E-5
15	2.726939828124878E-4	0.0	0.0	1.9478141629463417E-4	7.791256651785366E-5
16	3.1165026607141464E-4	0.0	0.0	4.2851911584819517E-4	7.791256651785366E-5
17	3.8956283258926834E-4	0.0	0.0	8.180819484374635E-4	7.791256651785366E-5
18	4.67475399107122E-4	0.0	0.0	9.739070814731709E-4	1.5582513303570732E-4
19	4.67475399107122E-4	0.0	0.0	0.0012855573475445854	1.5582513303570732E-4
20	4.67475399107122E-4	0.0	0.0	0.0013634699140624392	1.9478141629463417E-4
21	5.064316823660489E-4	3.895628325892683E-5	0.0	0.0016751201801338538	2.726939828124878E-4
22	5.453879656249756E-4	3.895628325892683E-5	0.0	0.002571114695089171	2.726939828124878E-4
23	5.453879656249756E-4	7.791256651785366E-5	0.0	0.0035839780598212684	3.1165026607141464E-4
24	5.843442488839025E-4	7.791256651785366E-5	0.0	0.006038223905133659	3.1165026607141464E-4
25	5.843442488839025E-4	7.791256651785366E-5	0.0	0.007362737535937172	3.1165026607141464E-4
26	5.843442488839025E-4	7.791256651785366E-5	0.0	0.01020654621383883	3.1165026607141464E-4
27	6.622568154017562E-4	7.791256651785366E-5	0.0	0.01795884658236527	3.5060654933034147E-4
28	6.622568154017562E-4	1.168688497767805E-4	0.0	0.03856672042633756	3.5060654933034147E-4
29	6.622568154017562E-4	1.168688497767805E-4	0.0	0.06875783995200586	3.5060654933034147E-4
30	7.012130986606829E-4	1.168688497767805E-4	0.0	0.1120772269359325	3.5060654933034147E-4
31	7.401693819196098E-4	1.168688497767805E-4	0.0	0.23958114204240002	3.5060654933034147E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	50	0.0070371884	18.5	5
CAGGACA	2395	0.0	17.843424	4
CGCGAAC	85	2.7252341E-5	17.411764	28
TGCATCA	2065	0.0	17.380146	14
GACAGGC	2195	0.0	17.362186	7
CCAGGAC	2390	0.0	17.338913	3
GCATCAG	2125	0.0	16.97647	15
GGTATCA	3045	0.0	16.950739	1
CTGCATC	2295	0.0	15.960784	13
GCTGCAT	2300	0.0	15.926086	12
ACAGGCT	2365	0.0	15.80127	8
GTATAGA	300	0.0	15.416667	1
TCCAGGA	2620	0.0	15.39313	2
ATCAAGC	2310	0.0	15.376623	30
CGCAAGA	425	0.0	14.8	2
TCTAGCG	315	0.0	14.68254	28
CTAGCGG	315	0.0	14.68254	29
AGAGGCC	2455	0.0	14.619144	23
GGACAGG	2690	0.0	14.579926	6
ACTGCGT	115	2.2118342E-5	14.478261	8
>>END_MODULE