FastQCFastQC Report
Fri 10 Feb 2017
ERR1630203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8243
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT600.7278903311901006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT530.6429697925512556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.31541914351571027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT260.31541914351571027No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC190.23049860487686522No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA160.19410408831736017No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA160.19410408831736017No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA150.18197258279752515No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA150.18197258279752515No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA140.16984107727769016No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA140.16984107727769016No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT140.16984107727769016No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA120.14557806623802014No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT110.13344656071818511No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA110.13344656071818511No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC110.13344656071818511No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA100.1213150551983501No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA100.1213150551983501No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG90.1091835496785151No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG90.1091835496785151No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.1091835496785151No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA90.1091835496785151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA650.001406649517.0769232
AACGCAG700.002310342215.8571437
GGTATCA750.003661160814.81
TCAACGC800.005623713.8750015
TATCAAC800.005623713.8750013
ATCAACG800.005623713.8750014
CAACGCA800.005623713.8750016
ACGCAGA850.00840483913.0588248
CAGAGTA850.00840483913.05882411
AGAGTAC850.00840483913.05882412
GCAGAGT850.00840483913.05882410
CGCAGAG850.00840483913.0588249
Produced by FastQC (version 0.11.5)