##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8243
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.440858910590805	31.0	31.0	34.0	30.0	34.0
2	31.612155768530876	31.0	31.0	34.0	30.0	34.0
3	31.639451655950502	31.0	31.0	34.0	28.0	34.0
4	35.34914472886085	37.0	35.0	37.0	33.0	37.0
5	35.28266407861216	37.0	35.0	37.0	33.0	37.0
6	35.411621982288004	37.0	35.0	37.0	33.0	37.0
7	35.40494965425209	37.0	35.0	37.0	33.0	37.0
8	35.34332160621133	37.0	35.0	37.0	33.0	37.0
9	36.98301589227223	39.0	37.0	39.0	33.0	39.0
10	36.98932427514254	39.0	37.0	39.0	33.0	39.0
11	36.99708843867524	39.0	37.0	39.0	33.0	39.0
12	36.908528448380444	39.0	37.0	39.0	33.0	39.0
13	36.95086740264467	39.0	37.0	39.0	33.0	39.0
14	37.96918597597962	40.0	37.0	41.0	33.0	41.0
15	37.99526871284726	40.0	37.0	41.0	33.0	41.0
16	37.82833919689433	39.0	37.0	41.0	33.0	41.0
17	37.881960451292	39.0	37.0	41.0	33.0	41.0
18	37.90816450321485	40.0	37.0	41.0	33.0	41.0
19	37.89518379230863	40.0	37.0	41.0	33.0	41.0
20	37.88098993085042	40.0	37.0	41.0	33.0	41.0
21	37.856241659589955	40.0	37.0	41.0	32.0	41.0
22	37.84119859274536	40.0	37.0	41.0	32.0	41.0
23	37.754579643333734	40.0	37.0	41.0	32.0	41.0
24	37.73298556350843	39.0	37.0	41.0	32.0	41.0
25	37.73237898823244	39.0	37.0	41.0	32.0	41.0
26	37.46536455174087	39.0	36.0	41.0	32.0	41.0
27	37.3012252820575	39.0	36.0	41.0	31.0	41.0
28	37.23996117918234	39.0	36.0	41.0	31.0	41.0
29	37.19386145820696	39.0	36.0	41.0	31.0	41.0
30	37.014921751789394	39.0	36.0	40.0	31.0	41.0
31	36.81572243115371	39.0	35.0	40.0	30.0	41.0
32	36.629261191313844	39.0	35.0	40.0	30.0	41.0
33	36.512313478102634	39.0	35.0	40.0	30.0	41.0
34	36.40058231226495	39.0	35.0	40.0	30.0	41.0
35	36.37583404100449	39.0	35.0	40.0	30.0	41.0
36	36.19701564964212	38.0	35.0	40.0	30.0	41.0
37	36.215940798253065	38.0	35.0	40.0	30.0	41.0
38	36.05920174693679	38.0	35.0	40.0	29.0	41.0
39	35.93133567875773	38.0	35.0	40.0	28.0	41.0
40	35.754822273444134	38.0	34.0	40.0	27.0	41.0
41	35.531238626713574	38.0	34.0	40.0	26.0	41.0
42	35.430304500788544	38.0	34.0	40.0	26.0	41.0
43	34.22952808443528	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	12.0
23	13.0
24	27.0
25	39.0
26	54.0
27	88.0
28	110.0
29	156.0
30	178.0
31	263.0
32	312.0
33	393.0
34	494.0
35	623.0
36	749.0
37	1129.0
38	1791.0
39	1807.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.85539245420357	18.221521290792186	14.048283391968944	23.874802863035303
2	21.169477132112092	21.242266165231104	32.71867038699502	24.869586315661774
3	21.13308261555259	22.285575639936916	32.269804682761134	24.311537061749362
4	14.42436006308383	17.991022685915322	34.35642363217275	33.228193618828094
5	14.181729952687128	37.01322334101662	34.39281814873226	14.412228557563994
6	31.83307048404707	33.84690040033968	15.83161470338469	18.488414412228558
7	27.162440858910593	31.954385539245422	19.79861700837074	21.08455659347325
8	28.193618828096568	32.90064296979256	19.20417323789882	19.70156496421206
9	26.48307654979983	13.174814994540823	19.228436248938493	41.11367220672085
10	21.86097294674269	25.17287395365765	27.320150430668445	25.646002668931217
11	35.24202353512071	21.824578430183188	18.779570544704598	24.153827489991507
12	20.76913744995754	28.096566783937888	26.106999878684945	25.027295887419626
13	33.58000727890331	18.379230862550042	25.063690403979134	22.977071454567515
14	23.88693436855514	21.64260584738566	24.61482469974524	29.855635084313963
15	29.52808443527842	25.245662986776658	21.727526386024508	23.498726191920415
16	23.64430425815844	25.43976707509402	25.015164381899798	25.90076428484775
17	21.81244692466335	28.424117432973432	23.60790974159893	26.15552590076429
18	22.05507703506005	23.26822758704355	27.42933398034696	27.247361397549437
19	23.425937158801407	26.616523110518013	26.798495693315537	23.159044037365035
20	25.99781632900643	22.46754822273444	29.540215940798255	21.994419507460876
21	28.145092806017225	23.583646730559263	25.294189008855998	22.977071454567515
22	28.775931093048644	24.068906951352663	23.8384083464758	23.31675360912289
23	27.13817784787092	24.602693194225402	23.717093291277447	24.542035666626226
24	23.450200169841075	25.90076428484775	26.301103967002305	24.347931578308867
25	24.46924663350722	26.070605362125438	25.888632779327914	23.571515225039427
26	25.973553317966758	25.17287395365765	25.12434793157831	23.729224796797283
27	24.78466577702293	24.88171782118161	25.43976707509402	24.893849326701446
28	23.717093291277447	25.36697804197501	26.774232682275873	24.141695984471674
29	25.063690403979134	25.41550406405435	27.07752032027175	22.44328521169477
30	24.444983622467547	26.762101176756037	27.162440858910593	21.630474341865828
31	24.5662986776659	24.17809050103118	26.15552590076429	25.10008492053864
32	22.92854543248817	25.60960815237171	26.11913138420478	25.342715030935338
33	22.746572849690647	25.985684823486594	27.853936673541185	23.41380565328157
34	22.552468761373287	25.354846536455174	26.373893000121313	25.718791702050225
35	23.5957782360791	26.179788911803954	26.798495693315537	23.425937158801407
36	22.24918112337741	26.62865461603785	25.233531481256822	25.888632779327914
37	22.382627684095596	26.677180638117193	26.034210845565937	24.905980832221278
38	22.576731772412955	24.542035666626226	26.349629989081645	26.53160257187917
39	22.212786606817904	23.74135630231712	29.552347446318088	24.493509644546887
40	21.897367463302196	25.476161591653522	28.76379958752881	23.862671357515467
41	21.096688098993084	24.42072061142788	29.80710906223462	24.675482227344414
42	20.4779813174815	24.651219216304742	30.243843260948683	24.626956205265074
43	20.344534756763313	24.93024384326095	29.625136479437096	25.10008492053864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.5
4	3.0
5	3.0
6	4.5
7	6.0
8	6.0
9	6.0
10	15.0
11	24.0
12	24.0
13	40.5
14	57.0
15	90.0
16	123.0
17	107.0
18	91.0
19	91.0
20	96.0
21	101.0
22	77.0
23	53.0
24	61.5
25	70.0
26	70.0
27	81.0
28	92.0
29	97.5
30	103.0
31	120.0
32	137.0
33	137.0
34	148.5
35	160.0
36	175.5
37	191.0
38	222.5
39	254.0
40	254.0
41	312.0
42	370.0
43	444.5
44	519.0
45	643.0
46	767.0
47	767.0
48	849.5
49	932.0
50	842.5
51	753.0
52	756.5
53	760.0
54	760.0
55	710.5
56	661.0
57	636.0
58	611.0
59	530.0
60	449.0
61	449.0
62	383.0
63	317.0
64	266.0
65	215.0
66	175.0
67	135.0
68	135.0
69	131.5
70	128.0
71	95.5
72	63.0
73	53.0
74	43.0
75	43.0
76	33.0
77	23.0
78	18.5
79	14.0
80	9.5
81	5.0
82	5.0
83	4.0
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8243.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.03845687249787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.14670658682635	72.24311537061749
2	6.7814371257485035	10.991144000970522
3	1.976047904191617	4.804076185854664
4	0.8383233532934131	2.7174572364430425
5	0.37425149700598803	1.5164381899793764
6	0.29940119760479045	1.4557806623802012
7	0.11976047904191617	0.6793643091107606
8	0.1347305389221557	0.8734683974281208
9	0.059880239520958084	0.4367341987140604
>10	0.23952095808383234	2.9115613247604024
>50	0.029940119760479042	1.3708601237413565
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.7278903311901006	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.6429697925512556	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.31541914351571027	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.31541914351571027	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	19	0.23049860487686522	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	16	0.19410408831736017	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	16	0.19410408831736017	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	15	0.18197258279752515	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	15	0.18197258279752515	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.16984107727769016	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	14	0.16984107727769016	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	14	0.16984107727769016	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	12	0.14557806623802014	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	11	0.13344656071818511	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	11	0.13344656071818511	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	11	0.13344656071818511	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	10	0.1213150551983501	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	10	0.1213150551983501	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	9	0.1091835496785151	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	9	0.1091835496785151	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1091835496785151	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	9	0.1091835496785151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.01213150551983501	0.0
19	0.0	0.0	0.0	0.01213150551983501	0.0
20	0.0	0.0	0.0	0.01213150551983501	0.0
21	0.0	0.0	0.0	0.01213150551983501	0.0
22	0.0	0.0	0.0	0.01213150551983501	0.0
23	0.0	0.0	0.0	0.01213150551983501	0.0
24	0.0	0.0	0.0	0.02426301103967002	0.0
25	0.0	0.0	0.0	0.02426301103967002	0.0
26	0.0	0.0	0.0	0.04852602207934004	0.0
27	0.0	0.0	0.0	0.06065752759917506	0.0
28	0.0	0.0	0.0	0.10918354967851511	0.0
29	0.0	0.0	0.0	0.16984107727769016	0.0
30	0.0	0.0	0.0	0.36394516559505036	0.0
31	0.0	0.0	0.0	0.7521533422297707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	65	0.0014066495	17.076923	2
AACGCAG	70	0.0023103422	15.857143	7
GGTATCA	75	0.0036611608	14.8	1
TCAACGC	80	0.0056237	13.875001	5
TATCAAC	80	0.0056237	13.875001	3
ATCAACG	80	0.0056237	13.875001	4
CAACGCA	80	0.0056237	13.875001	6
ACGCAGA	85	0.008404839	13.058824	8
CAGAGTA	85	0.008404839	13.058824	11
AGAGTAC	85	0.008404839	13.058824	12
GCAGAGT	85	0.008404839	13.058824	10
CGCAGAG	85	0.008404839	13.058824	9
>>END_MODULE