FastQCFastQC Report
Fri 10 Feb 2017
ERR1630202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692497
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158002.281598331833928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140072.0226802426580908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93771.3540852884561232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66670.9627478530592912No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA27170.3923482701008091No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25550.36895466695162576No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA21310.30772696488215834No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.2888099154220163No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19940.28794348567575023No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.2558855850639064No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.23292519678785611No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA11270.16274438734030616No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA11060.15971188322837498No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.15884545348210896No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10910.15754580886270989No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.15205842046969156No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA9220.1331413710095495No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9180.13256375117870547No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8730.1260655280817101No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.12274421405435693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG251.233119E-437.019
ATCTGCA200.001841430637.011
GCTTAAT250.005494853529.613
CGACGAG358.8658213E-426.4285724
TGCGACG358.8658213E-426.4285722
ACGAGTC358.8658213E-426.4285726
TGCACTA509.084275E-625.90000212
CCGGAAT659.380528E-825.6153853
ATTCACC451.3224705E-424.6666666
CTCGAAG451.3224705E-424.66666634
TCGAAGT451.3224705E-424.66666635
CGAAGTA451.3224705E-424.66666636
GACACCT701.9206345E-723.78571316
AGACACC701.9206345E-723.78571315
TAAGATA701.9206345E-723.7857134
ATGCTGC551.9012423E-523.5454546
CGGTAGA400.001930415923.12500214
GTTATAC400.001930415923.1250023
GCATCGT400.001930415923.1250028
TGCCCCG753.7363316E-722.29
Produced by FastQC (version 0.11.5)