##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630202.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 692497 Sequences flagged as poor quality 0 Sequence length 43 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.749600359279533 31.0 31.0 34.0 30.0 34.0 2 31.887782907362777 33.0 31.0 34.0 30.0 34.0 3 31.941748484108956 33.0 31.0 34.0 30.0 34.0 4 35.65161148712558 37.0 35.0 37.0 33.0 37.0 5 35.604212003806516 37.0 35.0 37.0 33.0 37.0 6 35.71826303940667 37.0 35.0 37.0 33.0 37.0 7 35.68789178870089 37.0 35.0 37.0 33.0 37.0 8 35.73148475733469 37.0 35.0 37.0 33.0 37.0 9 37.355982769600445 39.0 37.0 39.0 34.0 39.0 10 37.27570660955932 39.0 37.0 39.0 34.0 39.0 11 37.38150778992544 39.0 37.0 39.0 34.0 39.0 12 37.285648890897725 39.0 37.0 39.0 34.0 39.0 13 37.36712794423658 39.0 37.0 39.0 34.0 39.0 14 38.457252522393595 40.0 38.0 41.0 34.0 41.0 15 38.43799756533241 40.0 38.0 41.0 34.0 41.0 16 38.33758268988891 40.0 38.0 41.0 34.0 41.0 17 38.441915271835114 40.0 38.0 41.0 34.0 41.0 18 38.49911118748529 40.0 38.0 41.0 34.0 41.0 19 38.56504504712656 40.0 38.0 41.0 34.0 41.0 20 38.55227531671618 40.0 38.0 41.0 34.0 41.0 21 38.53013948074865 40.0 38.0 41.0 34.0 41.0 22 38.486166727076075 40.0 38.0 41.0 34.0 41.0 23 38.427848784904484 40.0 38.0 41.0 34.0 41.0 24 38.39627608495055 40.0 38.0 41.0 34.0 41.0 25 38.34839428907273 40.0 38.0 41.0 34.0 41.0 26 38.09699825414406 40.0 37.0 41.0 34.0 41.0 27 37.944471961611384 40.0 37.0 41.0 33.0 41.0 28 37.80700277401924 40.0 37.0 41.0 33.0 41.0 29 37.66358121407024 39.0 36.0 41.0 33.0 41.0 30 37.458783214945335 39.0 36.0 41.0 33.0 41.0 31 37.18393870298355 39.0 35.0 41.0 33.0 41.0 32 36.95607634401304 39.0 35.0 41.0 32.0 41.0 33 36.782287865507 39.0 35.0 41.0 31.0 41.0 34 36.63619481384035 39.0 35.0 41.0 31.0 41.0 35 36.440995412254495 39.0 35.0 40.0 31.0 41.0 36 36.22240529561861 38.0 35.0 40.0 30.0 41.0 37 36.024927183800074 38.0 35.0 40.0 30.0 41.0 38 35.796055434175166 38.0 35.0 40.0 29.0 41.0 39 35.52568458780327 38.0 35.0 40.0 27.0 41.0 40 35.232731694144526 38.0 35.0 40.0 25.0 41.0 41 34.87589837934316 38.0 34.0 40.0 23.0 41.0 42 34.58778449581731 38.0 34.0 40.0 21.0 41.0 43 33.54488467098052 37.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 5.0 11 13.0 12 12.0 13 4.0 14 5.0 15 4.0 16 4.0 17 13.0 18 22.0 19 47.0 20 139.0 21 286.0 22 496.0 23 897.0 24 1542.0 25 2615.0 26 3931.0 27 5776.0 28 8044.0 29 10554.0 30 13826.0 31 17303.0 32 22408.0 33 28439.0 34 37329.0 35 48041.0 36 66766.0 37 109341.0 38 148056.0 39 166577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08366101224987 21.204712800199857 13.687279511680195 20.024346675870074 2 18.49322090926026 24.750576536793663 33.753359220328754 23.002843333617328 3 20.287741318734955 27.916944044522936 30.938184569752647 20.85713006698946 4 13.883814659124877 18.405422694971964 35.149755161394204 32.561007484508956 5 11.580122368761165 41.1395284022891 33.95682580574356 13.323523423206165 6 28.645755866090393 36.322179013049876 17.553144634561594 17.478920486298136 7 25.28603011998608 34.12476877156146 23.664506849849168 16.92469425860329 8 28.445321784787513 33.02411418388816 20.233733864551038 18.296830166773287 9 26.920549836316983 13.518181306200605 23.09454048176382 36.466728375718596 10 19.802973875699102 29.439838728543226 30.629302365208805 20.12788503054887 11 29.386986514021 26.534844194270878 21.149116891481118 22.929052400227004 12 19.127447483527003 32.779780995441136 28.432614148508943 19.660157372522914 13 29.247346919914452 21.61872181395732 27.598675517727873 21.535255748400353 14 21.984932786712434 24.454546373486092 29.603305140672088 23.95721569912938 15 25.74841479457673 28.388281826491667 25.96097889232733 19.902324486604275 16 20.22117063323018 27.1739805370998 30.39117858994335 22.21367023972667 17 19.027663657748697 30.794646041787903 29.486192719968464 20.691497580494932 18 17.68715243531741 27.291814982591983 32.14136667740077 22.879665904689837 19 19.165137177489576 28.130374572019807 35.149033136605645 17.55545511388497 20 19.05827750878343 27.56344070804639 35.23553170627454 18.142750076895638 21 20.226513616665486 27.489505369698353 33.85704197996526 18.4269390336709 22 20.614096523161834 27.483440361474493 33.233212562653705 18.669250552709975 23 19.316329168213002 28.4962967348595 33.63884608886392 18.548528008063574 24 17.698849236892002 30.118397624827253 32.73154973956566 19.451203398715084 25 18.825063502080152 28.971966665559563 33.80101285637338 18.4019569759869 26 18.54838360310586 29.473918298563028 33.57299742814771 18.40470067018341 27 17.706647104608393 30.11435428601135 32.880286846008 19.29871176337226 28 18.155024498301074 29.93702499794223 34.58094403297054 17.327006470786156 29 18.821742188052802 29.59651810765967 33.700940220679655 17.88079948360787 30 17.882821153015826 31.129087923846598 33.37920597489953 17.608884948238043 31 18.45957455411359 30.37153951569465 33.58353899006061 17.58534694013115 32 16.86765430030744 30.996090957794763 33.54917061012539 18.587084131772414 33 16.62851969033801 31.39190494688064 33.708016063607495 18.27155929917386 34 17.717910691309854 30.604320307524794 32.969095895000265 18.70867310616508 35 17.815528442722496 31.34540655049769 32.7924886317197 18.04657637506011 36 17.770040881043528 31.35277120334095 31.501797119698715 19.375390795916804 37 17.48975085812646 30.58886897704972 32.63494282285699 19.286437341966824 38 17.42808994118386 31.218474592669715 32.47118760081271 18.882247865333714 39 16.635017913435004 31.212698394361276 33.27220190123567 18.88008179096805 40 17.239352661455573 30.859483867800147 33.9009410871094 18.000222383634874 41 16.55848328584817 30.955224354762546 33.282887868106286 19.203404491282996 42 15.961079975797729 31.400280434427874 33.266570107884945 19.372069481889454 43 16.60281560786545 30.643742860979906 33.23913316591985 19.514308365234793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 214.0 1 315.0 2 416.0 3 1255.5 4 2095.0 5 2095.0 6 3026.0 7 3957.0 8 3756.0 9 3555.0 10 4926.5 11 6298.0 12 6298.0 13 12183.0 14 18068.0 15 25678.5 16 33289.0 17 27650.0 18 22011.0 19 22011.0 20 25144.5 21 28278.0 22 19017.0 23 9756.0 24 9862.5 25 9969.0 26 9969.0 27 10677.5 28 11386.0 29 13538.5 30 15691.0 31 18838.0 32 21985.0 33 21985.0 34 26937.5 35 31890.0 36 34384.5 37 36879.0 38 39014.0 39 41149.0 40 41149.0 41 46249.5 42 51350.0 43 53018.5 44 54687.0 45 57118.5 46 59550.0 47 59550.0 48 60171.5 49 60793.0 50 54999.0 51 49205.0 52 43288.5 53 37372.0 54 37372.0 55 33515.5 56 29659.0 57 24307.0 58 18955.0 59 16236.5 60 13518.0 61 13518.0 62 11229.0 63 8940.0 64 6774.5 65 4609.0 66 3621.0 67 2633.0 68 2633.0 69 1999.0 70 1365.0 71 1043.0 72 721.0 73 681.5 74 642.0 75 642.0 76 561.0 77 480.0 78 435.0 79 390.0 80 324.5 81 259.0 82 259.0 83 230.5 84 202.0 85 162.0 86 122.0 87 120.5 88 119.0 89 119.0 90 77.5 91 36.0 92 19.5 93 3.0 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 692497.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.662747561657607 #Duplication Level Percentage of deduplicated Percentage of total 1 68.70137066599857 15.569618205433944 2 11.501607285105058 5.21316044911316 3 4.853889827274425 3.3000743954285436 4 2.7210583498624255 2.46666633933891 5 1.8296440714120799 2.0732380859047703 6 1.2972180595251235 1.7639115249264723 7 1.0040934375856714 1.5928861273024755 8 0.8071674503344355 1.4634105733532892 9 0.6494239022292128 1.3245956961054535 >10 5.418426860809946 25.299706303206083 >50 0.7717926554564087 12.133708152609813 >100 0.4213260153856325 16.423401492696062 >500 0.012767455011685835 1.8113035181909487 >1k 0.007660473007011502 2.930926355833101 >5k 0.0012767455011685833 2.321130482895813 >10k+ 0.0012767455011685833 4.31226229766115 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 15800 2.281598331833928 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 14007 2.0226802426580908 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 9377 1.3540852884561232 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6667 0.9627478530592912 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 2717 0.3923482701008091 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2555 0.36895466695162576 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 2131 0.30772696488215834 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2000 0.2888099154220163 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1994 0.28794348567575023 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1772 0.2558855850639064 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1613 0.23292519678785611 No Hit GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA 1127 0.16274438734030616 No Hit GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA 1106 0.15971188322837498 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1100 0.15884545348210896 No Hit GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA 1091 0.15754580886270989 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.15205842046969156 No Hit ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA 922 0.1331413710095495 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 918 0.13256375117870547 No Hit GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 873 0.1260655280817101 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.12274421405435693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.4440495771100812E-4 0.0 11 0.0 0.0 0.0 1.4440495771100812E-4 0.0 12 0.0 0.0 0.0 5.776198308440325E-4 0.0 13 0.0 0.0 0.0 0.001010834703977057 0.0 14 0.0 0.0 0.0 0.0012996446193990732 0.0 15 0.0 0.0 0.0 0.00231047932337613 0.0 16 0.0 0.0 0.0 0.004332148731330244 0.0 17 0.0 0.0 0.0 0.007075842927839399 0.0 18 0.0 0.0 0.0 0.007797867716394439 0.0 19 0.0 0.0 0.0 0.009675132166637545 0.0 20 0.0 0.0 0.0 0.012563231320857708 0.0 21 0.0 0.0 0.0 0.01545133047507787 0.0 22 0.0 0.0 0.0 0.02411562793773836 0.0 23 0.0 0.0 0.0 0.03379076010437591 0.0 24 0.0 0.0 0.0 0.04808685091776571 0.0 25 0.0 0.0 0.0 0.053141024437650994 0.0 26 0.0 0.0 0.0 0.06353818139284358 0.0 27 0.0 0.0 0.0 0.08115558623358657 0.0 28 0.0 0.0 0.0 0.11509075129567348 0.0 29 0.0 0.0 0.0 0.16808737077561348 0.0 30 0.0 0.0 0.0 0.2691708411733192 0.0 31 0.0 0.0 0.0 0.5296773848839779 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGCG 25 1.233119E-4 37.0 19 ATCTGCA 20 0.0018414306 37.0 11 GCTTAAT 25 0.0054948535 29.6 13 CGACGAG 35 8.8658213E-4 26.42857 24 TGCGACG 35 8.8658213E-4 26.42857 22 ACGAGTC 35 8.8658213E-4 26.42857 26 TGCACTA 50 9.084275E-6 25.900002 12 CCGGAAT 65 9.380528E-8 25.615385 3 ATTCACC 45 1.3224705E-4 24.666666 6 CTCGAAG 45 1.3224705E-4 24.666666 34 TCGAAGT 45 1.3224705E-4 24.666666 35 CGAAGTA 45 1.3224705E-4 24.666666 36 GACACCT 70 1.9206345E-7 23.785713 16 AGACACC 70 1.9206345E-7 23.785713 15 TAAGATA 70 1.9206345E-7 23.785713 4 ATGCTGC 55 1.9012423E-5 23.545454 6 CGGTAGA 40 0.0019304159 23.125002 14 GTTATAC 40 0.0019304159 23.125002 3 GCATCGT 40 0.0019304159 23.125002 8 TGCCCCG 75 3.7363316E-7 22.2 9 >>END_MODULE