FastQCFastQC Report
Fri 10 Feb 2017
ERR1630201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901903
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47940.25206332815080473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47630.2504333817234633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32930.1731423737172716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3350.019.3283585
TAATACG500.00703653618.4999984
GACGGAC3100.017.9032277
TAGTACT2000.017.5749994
CGCAAGA3500.016.9142862
GCGCAAG3700.016.5000021
CAAGACG3950.016.3924054
TAATACT3300.015.1363644
TCGTTTA2950.015.05084730
AGACGGA4000.014.7999996
CGCATCG3150.014.6825413
CGTTATT2400.014.6458332
CTAGTAC1152.2112372E-514.4782613
CGAATTA2450.014.34693815
ACGGACC4050.014.1604948
AATAACG3300.014.0151512
CGTTTAT3450.013.94202831
CTACGCA800.00630062213.87512
CGGTCGA1601.7850653E-713.87520
ATAATAC2405.456968E-1213.8749993
Produced by FastQC (version 0.11.5)