##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1901903
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817095824550464	31.0	31.0	34.0	30.0	34.0
2	32.00837529569068	33.0	31.0	34.0	30.0	34.0
3	32.159018099240605	34.0	31.0	34.0	30.0	34.0
4	35.80953445049511	37.0	35.0	37.0	35.0	37.0
5	35.72887103075183	37.0	35.0	37.0	35.0	37.0
6	35.79028162845319	37.0	35.0	37.0	35.0	37.0
7	35.75817115804539	37.0	35.0	37.0	35.0	37.0
8	35.74404215146619	37.0	35.0	37.0	33.0	37.0
9	37.45694549091095	39.0	37.0	39.0	35.0	39.0
10	37.36313523875823	39.0	37.0	39.0	34.0	39.0
11	37.45114340741878	39.0	37.0	39.0	35.0	39.0
12	37.32485726138504	39.0	37.0	39.0	34.0	39.0
13	37.37066085915002	39.0	37.0	39.0	34.0	39.0
14	38.570175240272505	40.0	38.0	41.0	34.0	41.0
15	38.56635012405995	40.0	38.0	41.0	34.0	41.0
16	38.54060222839966	40.0	38.0	41.0	34.0	41.0
17	38.50483647168126	40.0	38.0	41.0	34.0	41.0
18	38.4823106120554	40.0	38.0	41.0	34.0	41.0
19	38.48977997300599	40.0	38.0	41.0	34.0	41.0
20	38.44305782156083	40.0	38.0	41.0	34.0	41.0
21	38.3974345694812	40.0	38.0	41.0	34.0	41.0
22	38.374542234803776	40.0	38.0	41.0	34.0	41.0
23	38.31643990256075	40.0	38.0	41.0	34.0	41.0
24	38.302554336367315	40.0	38.0	41.0	34.0	41.0
25	38.26722340729259	40.0	38.0	41.0	34.0	41.0
26	38.10264719073476	40.0	38.0	41.0	33.0	41.0
27	37.99970608385391	40.0	37.0	41.0	33.0	41.0
28	37.911922427168996	40.0	37.0	41.0	33.0	41.0
29	37.83563935700191	40.0	37.0	41.0	33.0	41.0
30	37.78046514464723	40.0	37.0	41.0	33.0	41.0
31	37.67086439213777	40.0	37.0	41.0	32.0	41.0
32	37.56620763519486	40.0	37.0	41.0	32.0	41.0
33	37.49533703874488	40.0	37.0	41.0	32.0	41.0
34	37.38852033989115	40.0	36.0	41.0	31.0	41.0
35	37.30110052931196	39.0	36.0	41.0	31.0	41.0
36	37.195687687542424	39.0	36.0	41.0	31.0	41.0
37	37.12985625449878	39.0	36.0	41.0	31.0	41.0
38	37.01760289562612	39.0	36.0	41.0	31.0	41.0
39	36.91501564485676	39.0	36.0	41.0	31.0	41.0
40	36.79835512116023	39.0	35.0	41.0	30.0	41.0
41	36.67131972555909	39.0	35.0	41.0	30.0	41.0
42	36.59842010870165	39.0	35.0	40.0	30.0	41.0
43	35.372895988912155	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	4.0
12	2.0
13	5.0
14	2.0
15	3.0
16	16.0
17	31.0
18	79.0
19	133.0
20	322.0
21	669.0
22	1081.0
23	2007.0
24	3143.0
25	5057.0
26	7790.0
27	11369.0
28	16206.0
29	22544.0
30	30357.0
31	39668.0
32	51874.0
33	67577.0
34	90004.0
35	119618.0
36	168370.0
37	254285.0
38	450244.0
39	559435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87742329656139	18.621875037791096	12.044567993215216	26.456133672432298
2	19.27742897508443	20.621661567388035	33.47799546033631	26.622913997191233
3	20.198453864366375	22.059642368722272	29.60445406521784	28.137449701693512
4	14.991090502512483	15.33700719752795	34.040537293437154	35.631365006522415
5	15.527395456024834	35.54087669034646	33.525053591061166	15.406674262567543
6	35.38045841454585	34.39297377416198	15.24383735658443	14.982730454707733
7	29.93670024181044	30.10689819617509	20.538481720676607	19.41791984133786
8	28.046908806600545	32.55281683661049	19.527862356807894	19.87241199998107
9	27.19639224503037	13.611367141226447	19.126001694092707	40.06623891965047
10	17.70495130403601	25.914570827218842	32.30963934543455	24.0708385233106
11	37.037167510645915	21.047393058426216	20.57129096489148	21.344148466036387
12	21.97872341544232	24.89785230897685	28.512547695650092	24.610876579930732
13	30.974765800358906	18.90658987340574	24.15480705377719	25.96383727245816
14	23.180782616148143	20.401145589443836	24.407974539185226	32.010097255222796
15	26.342668369522528	26.776812487282477	21.38100628686111	25.499512856333894
16	25.531848890295667	25.72013399211211	23.41191953532856	25.336097582263662
17	24.23036295752202	25.704675790510873	24.981505365941377	25.083455886025735
18	24.68848306143899	24.295771130283722	25.814986358399985	25.200759449877307
19	25.085611621623183	24.914677562420376	25.55593003428671	24.443780781669727
20	25.49967059308493	24.229784589434896	25.431318000970606	24.83922681650957
21	25.78385963952946	24.479744760905263	25.0331904413632	24.703205158202074
22	25.711826523224367	24.57081144516834	25.143238114667255	24.57412391694003
23	25.100649191888337	24.31117675296795	25.31895685531807	25.26921719982565
24	24.984554943128014	24.839542290011636	25.187194089288468	24.988708677571886
25	25.42895194970511	24.380948975841566	25.094181985095982	25.095917089357343
26	25.15885405301953	25.0599005312048	24.95474269718277	24.826502718592906
27	25.13314296260114	24.850320967998893	24.935709129224783	25.080826940175182
28	24.77182064490145	25.00337819541796	25.43657589267171	24.788225267008883
29	24.41391595680747	25.133721330688264	25.777707906239172	24.674654806265092
30	24.540894041389073	25.54651840814174	25.488734178346633	24.423853372122554
31	25.180884619247145	25.049016695383518	24.868671010035737	24.9014276753336
32	24.560348240683147	24.94895901631156	25.218846597329097	25.271846145676196
33	24.28283671669901	24.79527084188836	25.745687345779466	25.176205095633165
34	25.114056815726144	24.647366348336377	25.185511563944114	25.05306527199337
35	24.823295404655234	24.83049871628574	25.82539698396816	24.520808895090866
36	24.376795241397694	25.185143511525037	25.291300344970274	25.146760902106998
37	24.852897334932432	24.211329389564032	25.877187217224012	25.058586058279523
38	24.21285417815735	24.38052834450548	26.297029869556965	25.10958760778021
39	24.336519790967255	24.318169748930412	26.377107560164742	24.968202899937587
40	24.15591121103442	24.290250343997567	26.871612274653334	24.68222617031468
41	23.646158610612634	24.19697534522002	27.130878914434646	25.025987129732698
42	23.98592357233781	24.408920959691425	26.912728987755948	24.692426480214817
43	23.525595153906377	23.87687489845697	27.20448939825007	25.393040549386587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	79.5
2	114.0
3	367.5
4	621.0
5	621.0
6	893.0
7	1165.0
8	1082.5
9	1000.0
10	1352.0
11	1704.0
12	1704.0
13	3343.0
14	4982.0
15	7686.0
16	10390.0
17	9588.0
18	8786.0
19	8786.0
20	11892.0
21	14998.0
22	14690.0
23	14382.0
24	17418.5
25	20455.0
26	20455.0
27	22821.0
28	25187.0
29	29066.5
30	32946.0
31	39238.5
32	45531.0
33	45531.0
34	53015.0
35	60499.0
36	70164.0
37	79829.0
38	85917.0
39	92005.0
40	92005.0
41	98808.0
42	105611.0
43	110592.5
44	115574.0
45	120884.5
46	126195.0
47	126195.0
48	131433.0
49	136671.0
50	136705.5
51	136740.0
52	138197.0
53	139654.0
54	139654.0
55	137058.0
56	134462.0
57	129900.0
58	125338.0
59	119331.0
60	113324.0
61	113324.0
62	104908.0
63	96492.0
64	86850.5
65	77209.0
66	67791.5
67	58374.0
68	58374.0
69	51054.5
70	43735.0
71	37598.5
72	31462.0
73	25420.0
74	19378.0
75	19378.0
76	15378.5
77	11379.0
78	9196.5
79	7014.0
80	5513.0
81	4012.0
82	4012.0
83	3031.0
84	2050.0
85	1634.0
86	1218.0
87	981.0
88	744.0
89	744.0
90	611.5
91	479.0
92	283.5
93	88.0
94	67.5
95	47.0
96	47.0
97	28.0
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1901903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.37204782459905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65639619842032	46.497069684294004
2	12.412066905501717	14.490355260201385
3	3.6201415898326887	6.3394523404060115
4	1.5002003200185934	3.5027905930641645
5	0.7827133842791122	2.2844291550047053
6	0.45550532145754613	1.5953267045087545
7	0.2803788185958168	1.145640006565572
8	0.20114459651637684	0.9392977606010903
9	0.14240079593459326	0.7481003463499543
>10	0.8022080776299817	8.722553062100415
>50	0.07289063039374344	3.0092054359832456
>100	0.06872971979945235	8.0293352117096
>500	0.004683264900776737	1.7948959085131153
>1k	5.403767193203927E-4	0.9015485306980088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4794	0.25206332815080473	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4763	0.2504333817234633	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3293	0.1731423737172716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.628945850550738E-4	0.0	0.0	0.0
6	0.0	2.628945850550738E-4	0.0	0.0	0.0
7	0.0	2.628945850550738E-4	0.0	0.0	0.0
8	0.0	2.628945850550738E-4	0.0	0.0	0.0
9	0.0	2.628945850550738E-4	0.0	0.0	0.0
10	0.0	2.628945850550738E-4	0.0	5.2578917011014754E-5	0.0
11	0.0	3.1547350206608855E-4	0.0	5.2578917011014754E-5	0.0
12	0.0	3.1547350206608855E-4	0.0	1.0515783402202951E-4	0.0
13	0.0	3.1547350206608855E-4	0.0	1.0515783402202951E-4	0.0
14	0.0	3.1547350206608855E-4	0.0	2.1031566804405902E-4	0.0
15	0.0	3.1547350206608855E-4	0.0	3.680524190771033E-4	0.0
16	0.0	3.1547350206608855E-4	0.0	5.783680871211623E-4	0.0
17	0.0	3.1547350206608855E-4	0.0	0.0012093150912533394	0.0
18	0.0	3.680524190771033E-4	0.0	0.0013670518422863836	0.0
19	0.0	3.680524190771033E-4	0.0	0.001735104261363487	0.0
20	0.0	3.680524190771033E-4	0.0	0.001945419929407546	0.0
21	0.0	3.680524190771033E-4	0.0	0.0031547350206608854	0.0
22	0.0	3.680524190771033E-4	0.0	0.005152733867079446	0.0
23	0.0	4.2063133608811803E-4	0.0	0.007676521883608155	0.0
24	0.0	4.732102530991328E-4	0.0	0.011251888240357158	0.0
25	0.0	4.732102530991328E-4	0.0	0.012408624414599483	0.0
26	0.0	4.732102530991328E-4	0.0	0.01582625402031544	0.0
27	0.0	4.732102530991328E-4	0.0	0.02465951207816592	0.0
28	0.0	6.835259211431918E-4	0.0	0.04800455123105647	0.0
29	0.0	7.886837551652214E-4	0.0	0.07949932252065431	0.0
30	0.0	7.886837551652214E-4	0.0	0.12445429656507193	0.0
31	0.0	7.886837551652214E-4	0.0	0.26736379300101004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	335	0.0	19.328358	5
TAATACG	50	0.007036536	18.499998	4
GACGGAC	310	0.0	17.903227	7
TAGTACT	200	0.0	17.574999	4
CGCAAGA	350	0.0	16.914286	2
GCGCAAG	370	0.0	16.500002	1
CAAGACG	395	0.0	16.392405	4
TAATACT	330	0.0	15.136364	4
TCGTTTA	295	0.0	15.050847	30
AGACGGA	400	0.0	14.799999	6
CGCATCG	315	0.0	14.68254	13
CGTTATT	240	0.0	14.645833	2
CTAGTAC	115	2.2112372E-5	14.478261	3
CGAATTA	245	0.0	14.346938	15
ACGGACC	405	0.0	14.160494	8
AATAACG	330	0.0	14.015151	2
CGTTTAT	345	0.0	13.942028	31
CTACGCA	80	0.006300622	13.875	12
CGGTCGA	160	1.7850653E-7	13.875	20
ATAATAC	240	5.456968E-12	13.874999	3
>>END_MODULE