FastQCFastQC Report
Fri 10 Feb 2017
ERR1630200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580436
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC31280.5389052367530615No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT16140.27806683251900294No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG12360.21294337360191307No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG11340.19537037675126973No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT11280.19433667105417307No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT9670.16659890151541254No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG9390.1617749415956281No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG9040.15574499169589756No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT8740.15057646321041424No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG8190.1411008276536948No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8040.13851656341095314No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC7910.13627686773391037No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG7800.13438174062256647No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT7710.1328311820769215No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG7630.13145290781412594No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT7370.12697351646004038No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG7280.1254229579143954No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC6960.11990986086321317No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6870.11835930231756818No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG6860.11818701803471873No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT6710.11560275379197706No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6460.11129564672074096No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT6420.11060650958934319No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC6250.10767767678090265No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC6080.10474884397246208No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA5900.10164772688117209No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT5870.10113087403262375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAG358.8640826E-426.4285721
CAGGACA7050.025.9787244
GACAGGC6750.024.9407427
CCAGGAC7450.024.0872483
ACAGGCT7000.024.0500018
CTAGCGG1002.0008883E-1124.0529
TGCATCA6850.024.03649514
GCATCAG6900.023.86231815
CTATATA400.001930041223.1250022
TGTATAC400.001930041223.1250028
TGCGCAC400.001930041223.12500210
GCTGCAT7150.023.02797312
GGTATCA3150.022.3174611
CTGCATC7550.022.29801213
ACATTAT502.6998698E-422.1999991
TCCAGGA8150.021.791412
CTATCGC603.722196E-521.58333430
TCGTGTA705.0947438E-621.14285913
ATAACAC450.003823561420.5555553
ATATTCT450.003823561420.5555554
Produced by FastQC (version 0.11.5)