##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580436
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.781929446140488	31.0	31.0	34.0	30.0	34.0
2	31.983972393166518	33.0	31.0	34.0	30.0	34.0
3	32.120676525921894	33.0	31.0	34.0	30.0	34.0
4	35.78611595421373	37.0	35.0	37.0	35.0	37.0
5	35.7052457118442	37.0	35.0	37.0	35.0	37.0
6	35.76020784375883	37.0	35.0	37.0	35.0	37.0
7	35.73130715531084	37.0	35.0	37.0	33.0	37.0
8	35.699246773115384	37.0	35.0	37.0	33.0	37.0
9	37.42166578227401	39.0	37.0	39.0	34.0	39.0
10	37.32755032423902	39.0	37.0	39.0	34.0	39.0
11	37.41446085356525	39.0	37.0	39.0	34.0	39.0
12	37.276940093309165	39.0	37.0	39.0	34.0	39.0
13	37.30279134995072	39.0	37.0	39.0	34.0	39.0
14	38.50652440579151	40.0	38.0	41.0	34.0	41.0
15	38.50506343507295	40.0	38.0	41.0	34.0	41.0
16	38.480197644529284	40.0	38.0	41.0	34.0	41.0
17	38.43057287969733	40.0	38.0	41.0	34.0	41.0
18	38.33323398273022	40.0	38.0	41.0	33.0	41.0
19	38.3385834097127	40.0	38.0	41.0	34.0	41.0
20	38.28098877395613	40.0	38.0	41.0	34.0	41.0
21	38.24230578392795	40.0	38.0	41.0	33.0	41.0
22	38.19473809343321	40.0	38.0	41.0	33.0	41.0
23	38.115526948707526	40.0	38.0	41.0	33.0	41.0
24	38.08173166378378	40.0	37.0	41.0	33.0	41.0
25	38.04569668318299	40.0	37.0	41.0	33.0	41.0
26	37.842866396984334	40.0	37.0	41.0	33.0	41.0
27	37.72968423736639	40.0	37.0	41.0	32.0	41.0
28	37.58766341164228	40.0	37.0	41.0	32.0	41.0
29	37.49851318663901	40.0	37.0	41.0	32.0	41.0
30	37.44444348730954	40.0	37.0	41.0	31.0	41.0
31	37.377052767230154	40.0	37.0	41.0	31.0	41.0
32	37.26643247489818	39.0	36.0	41.0	31.0	41.0
33	37.17859333328739	39.0	36.0	41.0	31.0	41.0
34	37.05724489866238	39.0	36.0	41.0	31.0	41.0
35	36.91550145063366	39.0	36.0	41.0	30.0	41.0
36	36.805180932953846	39.0	35.0	41.0	30.0	41.0
37	36.71695587454948	39.0	35.0	41.0	30.0	41.0
38	36.5991823387936	39.0	35.0	41.0	30.0	41.0
39	36.51794168521594	39.0	35.0	40.0	30.0	41.0
40	36.372656072331836	39.0	35.0	40.0	30.0	41.0
41	36.22928281498736	39.0	35.0	40.0	29.0	41.0
42	36.16091352018138	39.0	35.0	40.0	29.0	41.0
43	34.813545334886186	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	0.0
13	0.0
14	0.0
15	2.0
16	10.0
17	22.0
18	33.0
19	76.0
20	163.0
21	310.0
22	543.0
23	949.0
24	1378.0
25	2117.0
26	2983.0
27	4143.0
28	5761.0
29	7763.0
30	10248.0
31	13221.0
32	17380.0
33	22002.0
34	29412.0
35	39270.0
36	52395.0
37	76951.0
38	136057.0
39	157243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18529519189023	17.081125223108145	11.36697241384063	28.36660717116099
2	20.63466084116078	18.663039508231744	32.16306362803134	28.53923602257613
3	20.915139653639677	20.234961304950073	28.408989104741956	30.440909936668298
4	15.696648726130014	14.164869167315604	31.637768849623388	38.50071325693099
5	16.616991365111744	34.999552060864595	32.16582017655693	16.21763639746673
6	38.72967906883791	31.974412338311197	14.295460653715484	15.000447939135409
7	32.61858327188527	28.24239020322654	19.108394379397556	20.03063214549063
8	29.862379314859865	31.197754791225908	18.259205149232645	20.680660744681585
9	28.137296790688378	13.09377778084061	17.07078816613718	41.69813726233384
10	19.083930011232937	24.64940148440138	30.0530980159742	26.213570488391486
11	39.710148922534096	19.45830375786478	19.142162098836046	21.689385220765082
12	25.075805084453755	22.476035256255642	26.21236449841154	26.23579516087906
13	32.02868188740878	16.529815517989924	23.00374201462349	28.437760579977812
14	24.244533419705185	18.35447835764839	22.448469770999733	34.95251845164669
15	28.16761882446988	24.78602292070099	19.314446381685492	27.731911873143638
16	27.816848024588413	23.489583692258922	20.181208608701045	28.51235967445162
17	26.626535914381606	24.12514041169052	22.4581176908393	26.790205983088576
18	26.821044869718623	21.387543157212853	23.80262423419636	27.988787738872155
19	27.469178341798234	22.234320407417872	22.301511277729155	27.99498997305474
20	27.23504400140584	22.58526349158219	22.325803361610927	27.853889145401045
21	30.153884321441122	22.076680288610632	21.343438380803395	26.425997009144847
22	29.062635673872744	22.506529574319995	21.6981717191904	26.73266303261686
23	27.703140397907777	22.286177976555553	21.793272643323295	28.21740898221337
24	27.79858589060637	22.560282270569022	22.33458986003625	27.306541978788356
25	27.792039087858093	22.0866727770159	22.55408003638644	27.56720809873957
26	28.746321730561164	22.756686353017386	22.547016380789614	25.949975535631836
27	29.316927275358523	21.861152650765973	21.517100937915636	27.304819135959864
28	27.079988146841337	21.579123279741435	23.398273022348718	27.942615551068506
29	26.72714993556568	21.882171333273607	23.959575215872206	27.43110351528851
30	26.265944910377716	22.91656616750167	23.559531111095797	27.257957811024813
31	27.323253554224756	22.76495599859416	22.371630980848877	27.540159466332202
32	26.533502401642906	22.294447622132328	22.754274373057495	28.41777560316727
33	27.23469943284014	22.304784679103296	22.725502897821638	27.735012990234925
34	28.08492236870215	22.719472947921908	22.479997794761182	26.71560688861477
35	28.513565664431567	22.07461287721644	23.541613545679454	25.870207912672544
36	27.25072187114514	23.33538925910867	21.80705538595125	27.60683348379494
37	26.83517218091228	22.503945310077253	23.275951181525613	27.384931327484857
38	27.321530711396257	21.891819253113177	23.920122115099684	26.86652792039088
39	26.24871648209277	22.09804353968396	23.891522924146678	27.76171705407659
40	26.866700204673727	21.45387260610989	25.119048439448967	26.560378749767416
41	25.122494125105955	21.710231618989862	25.739444141989814	27.42783011391437
42	26.02543605151989	22.027751552281387	25.041692796449567	26.905119599749156
43	25.308733434866205	19.74033312888932	26.363113245904803	28.587820190339674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	11.0
2	11.0
3	27.0
4	43.0
5	43.0
6	56.5
7	70.0
8	62.5
9	55.0
10	83.5
11	112.0
12	112.0
13	188.5
14	265.0
15	446.0
16	627.0
17	621.5
18	616.0
19	616.0
20	814.0
21	1012.0
22	1131.0
23	1250.0
24	1626.5
25	2003.0
26	2003.0
27	2508.0
28	3013.0
29	3909.0
30	4805.0
31	6118.0
32	7431.0
33	7431.0
34	9188.5
35	10946.0
36	13462.5
37	15979.0
38	18274.5
39	20570.0
40	20570.0
41	22529.0
42	24488.0
43	26072.5
44	27657.0
45	29941.5
46	32226.0
47	32226.0
48	34171.5
49	36117.0
50	38876.0
51	41635.0
52	45576.0
53	49517.0
54	49517.0
55	50927.0
56	52337.0
57	50575.5
58	48814.0
59	45205.5
60	41597.0
61	41597.0
62	40552.5
63	39508.0
64	35036.5
65	30565.0
66	26355.0
67	22145.0
68	22145.0
69	18895.5
70	15646.0
71	14535.5
72	13425.0
73	13862.0
74	14299.0
75	14299.0
76	13166.0
77	12033.0
78	9105.0
79	6177.0
80	4014.5
81	1852.0
82	1852.0
83	1251.5
84	651.0
85	534.0
86	417.0
87	337.0
88	257.0
89	257.0
90	234.0
91	211.0
92	121.5
93	32.0
94	21.0
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	580436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.00496515718006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.64567936314089	42.82709212416875
2	8.003148061438162	8.003942799199368
3	2.1829330130076285	3.274724677677136
4	0.9992463824935552	1.9986912216011379
5	0.5915937422277417	1.4791312233651992
6	0.36839356691150843	1.1052904486523556
7	0.27985007915920046	0.9795725420312928
8	0.20394476943343143	0.8158600871606287
9	0.17129122323761803	0.7708870484755055
>10	1.214152134054114	12.114081381280032
>50	0.16712149389493328	5.748825092948746
>100	0.16021564668914703	15.802664607002468
>500	0.010704062602076635	3.6564910282933383
>1k	0.0017264617100123604	1.4227457181440766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3128	0.5389052367530615	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1614	0.27806683251900294	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1236	0.21294337360191307	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1134	0.19537037675126973	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1128	0.19433667105417307	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	967	0.16659890151541254	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	939	0.1617749415956281	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	904	0.15574499169589756	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	874	0.15057646321041424	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	819	0.1411008276536948	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	804	0.13851656341095314	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	791	0.13627686773391037	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	780	0.13438174062256647	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	771	0.1328311820769215	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	763	0.13145290781412594	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	737	0.12697351646004038	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	728	0.1254229579143954	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	696	0.11990986086321317	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	687	0.11835930231756818	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	686	0.11818701803471873	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	671	0.11560275379197706	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	646	0.11129564672074096	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	642	0.11060650958934319	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	625	0.10767767678090265	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	608	0.10474884397246208	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	590	0.10164772688117209	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	587	0.10113087403262375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	1.722842828494442E-4	0.0	0.0	0.0	0.0
12	1.722842828494442E-4	0.0	0.0	0.0	0.0
13	1.722842828494442E-4	0.0	0.0	0.0	1.722842828494442E-4
14	1.722842828494442E-4	0.0	0.0	0.0	1.722842828494442E-4
15	1.722842828494442E-4	0.0	0.0	0.0	1.722842828494442E-4
16	1.722842828494442E-4	0.0	0.0	1.722842828494442E-4	1.722842828494442E-4
17	1.722842828494442E-4	0.0	0.0	3.445685656988884E-4	1.722842828494442E-4
18	1.722842828494442E-4	0.0	0.0	3.445685656988884E-4	1.722842828494442E-4
19	1.722842828494442E-4	0.0	0.0	5.168528485483327E-4	1.722842828494442E-4
20	1.722842828494442E-4	0.0	0.0	6.891371313977768E-4	1.722842828494442E-4
21	1.722842828494442E-4	0.0	0.0	6.891371313977768E-4	1.722842828494442E-4
22	1.722842828494442E-4	0.0	0.0	0.0018951271113438863	1.722842828494442E-4
23	1.722842828494442E-4	0.0	0.0	0.00327340137413944	1.722842828494442E-4
24	1.722842828494442E-4	0.0	0.0	0.004134822788386661	1.722842828494442E-4
25	1.722842828494442E-4	0.0	0.0	0.005168528485483327	1.722842828494442E-4
26	1.722842828494442E-4	0.0	0.0	0.0077527927282249895	1.722842828494442E-4
27	1.722842828494442E-4	0.0	0.0	0.01671157543639609	1.722842828494442E-4
28	1.722842828494442E-4	0.0	0.0	0.04634447208650049	1.722842828494442E-4
29	1.722842828494442E-4	0.0	0.0	0.0892432585160121	1.722842828494442E-4
30	1.722842828494442E-4	0.0	0.0	0.15333301173600536	1.722842828494442E-4
31	1.722842828494442E-4	0.0	0.0	0.30184206355222626	1.722842828494442E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTAAG	35	8.8640826E-4	26.428572	1
CAGGACA	705	0.0	25.978724	4
GACAGGC	675	0.0	24.940742	7
CCAGGAC	745	0.0	24.087248	3
ACAGGCT	700	0.0	24.050001	8
CTAGCGG	100	2.0008883E-11	24.05	29
TGCATCA	685	0.0	24.036495	14
GCATCAG	690	0.0	23.862318	15
CTATATA	40	0.0019300412	23.125002	2
TGTATAC	40	0.0019300412	23.125002	8
TGCGCAC	40	0.0019300412	23.125002	10
GCTGCAT	715	0.0	23.027973	12
GGTATCA	315	0.0	22.317461	1
CTGCATC	755	0.0	22.298012	13
ACATTAT	50	2.6998698E-4	22.199999	1
TCCAGGA	815	0.0	21.79141	2
CTATCGC	60	3.722196E-5	21.583334	30
TCGTGTA	70	5.0947438E-6	21.142859	13
ATAACAC	45	0.0038235614	20.555555	3
ATATTCT	45	0.0038235614	20.555555	4
>>END_MODULE