FastQCFastQC Report
Fri 10 Feb 2017
ERR1630199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338377
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC59270.2534663999859732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52720.22545551893471413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.1988131084080967No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT41120.1758484624164538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34240.14642634613665803No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT27670.11832993567760888No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG26230.11217181831672139No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG23590.10088193648842766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.018.4531631
CGCAAGA5300.017.1037732
TTAGACT2850.016.8771934
CAGGACA19400.016.6881454
AATCGTC1005.883023E-616.65000228
CTAATCG1005.883023E-616.65000226
GACAGGC17400.016.5862087
AAGACGG5250.016.5619055
ACGGACC5200.016.3653858
GACGGAC5200.016.3653857
CGGACCA5400.016.101859
CCAGGAC20000.015.81753
TGCATCA18400.015.78532614
TCTAGCG3000.015.41666728
AATGCGT1455.35274E-815.31034635
ATTAGAC2550.015.2352943
ACAGGCT20300.015.1280798
TGCGTTA1353.9769657E-715.07407337
TCCAGGA22050.014.7664392
ATGCGTT1406.001992E-714.53571536
Produced by FastQC (version 0.11.5)