##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630199.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2338377
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47213644335366	31.0	31.0	34.0	30.0	34.0
2	31.69198978607812	31.0	31.0	34.0	30.0	34.0
3	31.82103313537552	31.0	31.0	34.0	30.0	34.0
4	35.55508072479331	37.0	35.0	37.0	33.0	37.0
5	35.41003054682799	37.0	35.0	37.0	33.0	37.0
6	35.465541698366	37.0	35.0	37.0	33.0	37.0
7	35.45588842175577	37.0	35.0	37.0	33.0	37.0
8	35.43138296348279	37.0	35.0	37.0	33.0	37.0
9	37.094103303274025	39.0	37.0	39.0	33.0	39.0
10	36.9514783116666	39.0	37.0	39.0	33.0	39.0
11	37.05267970049312	39.0	37.0	39.0	33.0	39.0
12	36.91648523741039	39.0	37.0	39.0	33.0	39.0
13	36.961640488253174	39.0	37.0	39.0	33.0	39.0
14	38.09285628450844	40.0	37.0	41.0	33.0	41.0
15	38.06562158283288	40.0	37.0	41.0	33.0	41.0
16	38.043512230919134	40.0	37.0	41.0	33.0	41.0
17	37.99316748325869	40.0	37.0	41.0	33.0	41.0
18	37.921685853051066	40.0	37.0	41.0	33.0	41.0
19	37.92688433045655	40.0	37.0	41.0	33.0	41.0
20	37.87505949639429	40.0	37.0	41.0	33.0	41.0
21	37.82448724050912	40.0	37.0	41.0	33.0	41.0
22	37.78475369882615	39.0	37.0	41.0	33.0	41.0
23	37.72908645611892	39.0	37.0	41.0	33.0	41.0
24	37.70042640686254	39.0	37.0	41.0	32.0	41.0
25	37.672285093464396	39.0	37.0	41.0	32.0	41.0
26	37.473937692681716	39.0	37.0	41.0	32.0	41.0
27	37.373384616766245	39.0	36.0	40.0	32.0	41.0
28	37.27078524976939	39.0	36.0	40.0	31.0	41.0
29	37.18974185941788	39.0	36.0	40.0	31.0	41.0
30	37.13094894450296	39.0	36.0	40.0	31.0	41.0
31	37.03653730771386	39.0	36.0	40.0	31.0	41.0
32	36.932049023745954	39.0	36.0	40.0	31.0	41.0
33	36.85837912364003	39.0	36.0	40.0	30.0	41.0
34	36.746171810619074	39.0	35.0	40.0	30.0	41.0
35	36.632661029423396	39.0	35.0	40.0	30.0	41.0
36	36.53261642583724	39.0	35.0	40.0	30.0	41.0
37	36.448284002109155	38.0	35.0	40.0	30.0	41.0
38	36.335993725562645	38.0	35.0	40.0	30.0	41.0
39	36.242652916959074	38.0	35.0	40.0	30.0	41.0
40	36.109627746082005	38.0	35.0	40.0	29.0	41.0
41	35.98280901668123	38.0	35.0	40.0	29.0	41.0
42	35.918809926714125	38.0	35.0	40.0	29.0	41.0
43	34.75024386572396	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	4.0
13	3.0
14	6.0
15	15.0
16	45.0
17	97.0
18	184.0
19	359.0
20	677.0
21	1261.0
22	2145.0
23	3684.0
24	5825.0
25	8712.0
26	12913.0
27	18393.0
28	25458.0
29	34307.0
30	45977.0
31	59873.0
32	77462.0
33	100722.0
34	132799.0
35	177487.0
36	248627.0
37	376035.0
38	616342.0
39	388958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02940757628047	19.07237370193087	12.346640426244356	26.551578295544303
2	19.657865262958026	20.38884234663615	33.73861443214674	26.21467795825908
3	20.71364027271907	22.35482131409948	29.153981586373796	27.777556826807654
4	15.683057094728522	15.439341047230624	33.51692220715479	35.36067965088606
5	15.63858180267767	35.70480722312955	33.19871004547171	15.457900928721074
6	35.91478191925425	33.9999923023533	15.094828592652082	14.990397185740367
7	30.238964888895158	29.58428003696581	20.744259800707926	19.4324952734311
8	27.734578299393124	32.990531466910596	19.270074928037694	20.004815305658582
9	27.095417034977682	13.757105890110962	19.209904989657357	39.937572085254004
10	17.99649928133915	25.86541862154819	32.13335574203817	24.00472635507448
11	36.88040893320453	21.06807413860126	20.71034739051915	21.34116953767506
12	22.520021365246066	24.443406687629924	28.362663505499754	24.673908441624253
13	30.475068819099743	18.887330828176978	24.412829924344962	26.224770428378314
14	22.959172109544355	20.308145350386187	24.571059328756657	32.16162321131281
15	26.12765178583265	26.836091870558082	21.35883136038372	25.677424983225546
16	25.544212930592458	25.571111929342443	22.96802440325063	25.916650736814468
17	24.264137048901866	25.921354854242924	24.937766664656724	24.876741432198486
18	24.460170451556785	24.109286056097883	25.850408210481028	25.5801352818643
19	24.98249854493095	24.57371074039815	25.512609814414017	24.931180900256887
20	24.996183250177367	24.624900090960526	25.33141576401068	25.047500894851428
21	26.457324888159604	24.3329882221729	24.697257969950954	24.512428919716537
22	25.881883032547787	24.65175632500662	24.898423137073276	24.567937505372317
23	25.14834006663596	24.631742443583736	24.891281431522806	25.3286360582575
24	25.322820058527775	24.799294553444547	24.968086839718318	24.90979854830936
25	25.450515464358396	24.35992998562678	25.255337355781382	24.934217194233437
26	25.48802010967436	24.974244957079208	25.186058535471396	24.351676397775037
27	25.80105774218614	24.568664505338532	24.56049644689458	25.06978130558075
28	24.75742790833129	24.23073781515983	25.778734566752924	25.233099709755958
29	24.57610556381627	24.518415978261846	25.961254322977005	24.94422413494488
30	24.513412507906125	24.960945134167844	25.68067510072157	24.844967257204463
31	24.95051054641745	24.800748553376977	25.198588593712646	25.050152306492922
32	24.338718692494837	24.697685616989904	25.40159264310246	25.5620030474128
33	24.816143846779198	24.61946897356585	25.46813452236316	25.09625265729179
34	25.357502233386658	24.649532560404076	25.198246476081486	24.794718730127776
35	25.448206170348065	24.568407917115163	25.773004096430984	24.210381816105787
36	24.95743842844845	25.029539719215503	24.68224755888379	25.33077429345225
37	24.57315479924751	24.307970870394293	25.730581510167095	25.3882928201911
38	24.978222074541446	24.254728814044952	25.991574498038595	24.775474613375003
39	24.15158034825009	24.198535993126857	26.205398017513858	25.4444856411092
40	24.467568745330627	23.849490479935444	27.04414215500751	24.63879861972642
41	23.565361787256716	23.84491465661867	27.398148373850752	25.191575182273855
42	23.869889243693382	24.280900812828726	26.740341698537062	25.10886824494083
43	23.724489250450205	22.98495922599307	27.508224721676616	25.782326801880107
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	363.0
1	303.0
2	243.0
3	493.5
4	744.0
5	744.0
6	1031.5
7	1319.0
8	1282.0
9	1245.0
10	1782.5
11	2320.0
12	2320.0
13	4245.5
14	6171.0
15	8924.5
16	11678.0
17	11367.0
18	11056.0
19	11056.0
20	14532.5
21	18009.0
22	18170.0
23	18331.0
24	21754.0
25	25177.0
26	25177.0
27	29421.5
28	33666.0
29	39807.5
30	45949.0
31	53287.5
32	60626.0
33	60626.0
34	70594.5
35	80563.0
36	90255.0
37	99947.0
38	106851.5
39	113756.0
40	113756.0
41	120884.0
42	128012.0
43	132716.0
44	137420.0
45	141240.5
46	145061.0
47	145061.0
48	148289.5
49	151518.0
50	156045.5
51	160573.0
52	166283.0
53	171993.0
54	171993.0
55	171440.0
56	170887.0
57	163639.0
58	156391.0
59	143655.0
60	130919.0
61	130919.0
62	124387.5
63	117856.0
64	103364.0
65	88872.0
66	75702.5
67	62533.0
68	62533.0
69	54756.5
70	46980.0
71	42429.5
72	37879.0
73	38420.5
74	38962.0
75	38962.0
76	35687.0
77	32412.0
78	25079.0
79	17746.0
80	11645.5
81	5545.0
82	5545.0
83	3866.0
84	2187.0
85	1836.5
86	1486.0
87	1256.0
88	1026.0
89	1026.0
90	901.0
91	776.0
92	448.0
93	120.0
94	84.5
95	49.0
96	49.0
97	28.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2338377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.16772116747138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.56155874358713	41.73319095776204
2	10.847048132374217	11.100374686549303
3	3.1344174068160013	4.811429876832896
4	1.3205913375758196	2.7028659734903044
5	0.717356757714897	1.835275527817859
6	0.42660899082928166	1.3097165934173423
7	0.30572240467575523	1.0950183129968527
8	0.21364826930485953	0.8745516057363111
9	0.15693922151832915	0.7227200094200901
>10	1.0687235373869834	10.555958329839516
>50	0.12636830397699406	4.510046018224102
>100	0.10565324412800098	10.864683131677614
>500	0.011333840246437297	4.010472890322212
>1k	0.0038619011210082547	3.392614605834322
>5k	1.6790874439166325E-4	0.48108148007924645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5927	0.2534663999859732	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5272	0.22545551893471413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4649	0.1988131084080967	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4112	0.1758484624164538	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3424	0.14642634613665803	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2767	0.11832993567760888	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2623	0.11217181831672139	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2359	0.10088193648842766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.276470389505199E-5	0.0	0.0	0.0
3	0.0	4.276470389505199E-5	0.0	0.0	4.276470389505199E-5
4	0.0	4.276470389505199E-5	0.0	0.0	4.276470389505199E-5
5	0.0	4.276470389505199E-5	0.0	0.0	4.276470389505199E-5
6	0.0	4.276470389505199E-5	0.0	0.0	4.276470389505199E-5
7	0.0	4.276470389505199E-5	0.0	0.0	4.276470389505199E-5
8	0.0	4.276470389505199E-5	0.0	0.0	4.276470389505199E-5
9	0.0	4.276470389505199E-5	0.0	8.552940779010399E-5	4.276470389505199E-5
10	0.0	4.276470389505199E-5	0.0	1.2829411168515598E-4	4.276470389505199E-5
11	0.0	4.276470389505199E-5	0.0	1.2829411168515598E-4	4.276470389505199E-5
12	0.0	4.276470389505199E-5	0.0	1.2829411168515598E-4	4.276470389505199E-5
13	0.0	4.276470389505199E-5	0.0	1.2829411168515598E-4	4.276470389505199E-5
14	0.0	4.276470389505199E-5	0.0	2.1382351947525998E-4	4.276470389505199E-5
15	4.276470389505199E-5	4.276470389505199E-5	0.0	2.9935292726536396E-4	4.276470389505199E-5
16	4.276470389505199E-5	8.552940779010399E-5	0.0	4.2764703895051997E-4	4.276470389505199E-5
17	4.276470389505199E-5	8.552940779010399E-5	0.0	6.842352623208319E-4	4.276470389505199E-5
18	4.276470389505199E-5	8.552940779010399E-5	0.0	7.269999662158839E-4	4.276470389505199E-5
19	4.276470389505199E-5	8.552940779010399E-5	0.0	8.552940779010399E-4	4.276470389505199E-5
20	4.276470389505199E-5	8.552940779010399E-5	0.0	0.001154647005166404	1.2829411168515598E-4
21	4.276470389505199E-5	8.552940779010399E-5	0.0	0.0012829411168515598	2.1382351947525998E-4
22	4.276470389505199E-5	8.552940779010399E-5	0.0	0.0022237646025427037	2.1382351947525998E-4
23	4.276470389505199E-5	8.552940779010399E-5	0.0	0.0030362939765486915	2.1382351947525998E-4
24	4.276470389505199E-5	8.552940779010399E-5	0.0	0.004832411540140876	2.1382351947525998E-4
25	4.276470389505199E-5	8.552940779010399E-5	0.0	0.005987058545307279	2.5658822337031197E-4
26	4.276470389505199E-5	8.552940779010399E-5	0.0	0.009622058376386699	2.9935292726536396E-4
27	4.276470389505199E-5	8.552940779010399E-5	0.0	0.023263998918908284	2.9935292726536396E-4
28	4.276470389505199E-5	8.552940779010399E-5	0.0	0.05007746826110589	3.84882335055468E-4
29	4.276470389505199E-5	8.552940779010399E-5	0.0	0.08869399587833784	3.84882335055468E-4
30	4.276470389505199E-5	8.552940779010399E-5	0.0	0.1445874638691708	3.84882335055468E-4
31	4.276470389505199E-5	8.552940779010399E-5	0.0	0.29109933941361893	3.84882335055468E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1975	0.0	18.453163	1
CGCAAGA	530	0.0	17.103773	2
TTAGACT	285	0.0	16.877193	4
CAGGACA	1940	0.0	16.688145	4
AATCGTC	100	5.883023E-6	16.650002	28
CTAATCG	100	5.883023E-6	16.650002	26
GACAGGC	1740	0.0	16.586208	7
AAGACGG	525	0.0	16.561905	5
ACGGACC	520	0.0	16.365385	8
GACGGAC	520	0.0	16.365385	7
CGGACCA	540	0.0	16.10185	9
CCAGGAC	2000	0.0	15.8175	3
TGCATCA	1840	0.0	15.785326	14
TCTAGCG	300	0.0	15.416667	28
AATGCGT	145	5.35274E-8	15.310346	35
ATTAGAC	255	0.0	15.235294	3
ACAGGCT	2030	0.0	15.128079	8
TGCGTTA	135	3.9769657E-7	15.074073	37
TCCAGGA	2205	0.0	14.766439	2
ATGCGTT	140	6.001992E-7	14.535715	36
>>END_MODULE