FastQCFastQC Report
Fri 10 Feb 2017
ERR1630198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604612
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG93450.25925120373565863No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT81130.22507276788736205No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC67150.18628912071535023No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG63340.17571932846031696No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA62290.17280639358688257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56150.155772660136514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.14442608524856487No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC50370.13973764721418006No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT48940.13577050733893137No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC47420.131553687331674No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA47040.13049948232985964No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC45580.12644911574394138No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC45340.12578330205858496No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC44880.12450715916165178No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC43810.12153873981443773No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT43320.12017937020683502No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC42560.11807096020320634No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA42530.11798773349253677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42450.11776579559741797No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT42020.11657287941115439No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA39700.11013668045270891No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA38190.10594760268234141No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG36920.1024243385973303No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG36300.10070431991015955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.022.2369081
GACGGAC5400.018.1574087
CTTATAC13500.017.95185337
CGGACCA5450.017.6513779
CAAGACG6550.017.511454
CGGCCTT16950.017.46312524
AAGACGG5700.017.2017565
ACGGACC5500.017.1545478
TTCCTCG18800.016.13829619
TCGGCCT19150.016.03655223
GCGCAAG7050.016.0070921
CGCAAGA6250.015.6882
TCTAGCG4150.015.60240928
ATCTCGC19250.015.08831211
TCGCCAT15700.015.08280313
TCCTCGG20000.015.07749920
AGACGGA6700.014.9104486
GTACGTA1252.9628081E-614.79999913
CCTATAC4900.014.7244893
TCTCGCC19850.014.63224212
Produced by FastQC (version 0.11.5)