FastQCFastQC Report
Fri 10 Feb 2017
ERR1630197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629200
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102630.3903468735737106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82400.31340331659820475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64130.24391449870683096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43580.16575384147269132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.016.9583341
GTACTAG1504.6784407E-916.0333331
TACTAGG2300.015.2826092
TACGGGT855.3674134E-415.2352944
TATCGGT1152.2118742E-514.47826113
GTAAACG2551.8189894E-1213.78431327
TGCGCGA1000.001833571512.95000131
TTAGACT2305.7661964E-1012.8695654
GTATCAA60550.012.4962832
CTTATAC10150.012.39408937
CTAGACT2255.7152647E-912.3333334
GTACGAC1050.0026451312.3333333
TAGACAG5000.012.215
TCTTATA16650.012.11111137
TATCCCG1257.2241324E-411.845
AAGACGG4600.011.6630445
TAAACGC2705.802576E-1011.64814828
AACGCAG66200.011.5415417
CGCGATA1451.9400197E-411.482758534
GTATTAG5500.011.4363641
Produced by FastQC (version 0.11.5)