##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2629200
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873076220903698	33.0	31.0	34.0	30.0	34.0
2	32.06227407576449	33.0	31.0	34.0	30.0	34.0
3	32.204289898067856	34.0	31.0	34.0	30.0	34.0
4	35.84054008823977	37.0	35.0	37.0	35.0	37.0
5	35.75476076373041	37.0	35.0	37.0	35.0	37.0
6	35.817639205842084	37.0	35.0	37.0	35.0	37.0
7	35.790821162330744	37.0	35.0	37.0	35.0	37.0
8	35.78197208276282	37.0	35.0	37.0	35.0	37.0
9	37.48876958770729	39.0	37.0	39.0	35.0	39.0
10	37.401957249353416	39.0	37.0	39.0	34.0	39.0
11	37.4832477559714	39.0	37.0	39.0	35.0	39.0
12	37.360968355393275	39.0	37.0	39.0	34.0	39.0
13	37.403127947664686	39.0	37.0	39.0	34.0	39.0
14	38.63086338049597	40.0	38.0	41.0	34.0	41.0
15	38.622725543891676	40.0	38.0	41.0	34.0	41.0
16	38.602533470257114	40.0	38.0	41.0	34.0	41.0
17	38.57204168568386	40.0	38.0	41.0	34.0	41.0
18	38.55267267609919	40.0	38.0	41.0	34.0	41.0
19	38.56517990263198	40.0	38.0	41.0	34.0	41.0
20	38.51846074851666	40.0	38.0	41.0	34.0	41.0
21	38.48361973223794	40.0	38.0	41.0	34.0	41.0
22	38.45048874182261	40.0	38.0	41.0	34.0	41.0
23	38.3972272934733	40.0	38.0	41.0	34.0	41.0
24	38.38024456108322	40.0	38.0	41.0	34.0	41.0
25	38.34523581317511	40.0	38.0	41.0	34.0	41.0
26	38.187566560170396	40.0	38.0	41.0	34.0	41.0
27	38.09138977635783	40.0	38.0	41.0	33.0	41.0
28	38.01125551498555	40.0	37.0	41.0	33.0	41.0
29	37.938022972767385	40.0	37.0	41.0	33.0	41.0
30	37.87153240529439	40.0	37.0	41.0	33.0	41.0
31	37.76133919062833	40.0	37.0	41.0	33.0	41.0
32	37.6600604746691	40.0	37.0	41.0	33.0	41.0
33	37.58607180891526	40.0	37.0	41.0	32.0	41.0
34	37.48847063745626	40.0	37.0	41.0	32.0	41.0
35	37.40368781378366	40.0	36.0	41.0	32.0	41.0
36	37.29248973071657	40.0	36.0	41.0	31.0	41.0
37	37.21497261524418	39.0	36.0	41.0	31.0	41.0
38	37.11429256047467	39.0	36.0	41.0	31.0	41.0
39	37.001400806328924	39.0	36.0	41.0	31.0	41.0
40	36.886042522440285	39.0	35.0	41.0	31.0	41.0
41	36.75247375627567	39.0	35.0	41.0	30.0	41.0
42	36.66208466453674	39.0	35.0	41.0	30.0	41.0
43	35.511125817739234	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	9.0
12	5.0
13	3.0
14	4.0
15	11.0
16	16.0
17	41.0
18	72.0
19	186.0
20	377.0
21	812.0
22	1415.0
23	2670.0
24	4274.0
25	6738.0
26	10221.0
27	15163.0
28	21470.0
29	30286.0
30	40938.0
31	53820.0
32	69217.0
33	90088.0
34	119958.0
35	161189.0
36	224878.0
37	344295.0
38	611696.0
39	819334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11615700593337	18.821010193214665	12.179294081849992	25.88353871900198
2	19.179788528830063	20.555225924235508	33.770006085501294	26.49497946143314
3	20.339570972158832	22.65780465540849	29.111060398600337	27.891563973832344
4	14.926213296820325	15.504564125969878	33.48265632131447	36.08656625589533
5	15.130229727673816	36.084055986611894	33.542294233987526	15.24342005172676
6	35.45705918150008	33.907538414726915	15.480146052031035	15.155256351741976
7	29.791343374410467	30.040620721131905	20.95447284345048	19.21356306100715
8	27.860413814087938	33.07576449109996	19.33063289213449	19.73318880267762
9	26.82983417008976	13.947360413814089	19.52689030883919	39.695915107256965
10	17.61311425528678	26.260041077133728	32.617868553172066	23.508976114407425
11	36.35828388863533	21.533051878898522	20.80275368933516	21.30591054313099
12	21.58462650235813	25.350258633804962	29.117526243724328	23.94758862011258
13	30.607561235356762	19.5758785942492	24.509774836452152	25.306785333941882
14	22.88608702266849	20.546896394340482	24.93469496424768	31.63232161874334
15	25.799216491708503	27.44724631066484	21.828579035448044	24.92495816217861
16	25.286360870226687	25.777308687053097	24.169595314164006	24.766735128556213
17	23.84485775140727	26.254488057203712	25.307469952837362	24.59318423855165
18	24.123003194888177	24.778753993610223	26.419937623611744	24.67830518788985
19	24.492507226532787	25.137798569907194	26.391602008215425	23.97809219534459
20	24.577780313403316	24.72660885440438	26.479993914498706	24.215616917693595
21	25.04024037730108	24.766050509660733	26.10786551042142	24.085843602616766
22	24.869580100410772	24.71881180587251	26.216225467822913	24.195382625893807
23	24.426137228054163	24.881104518484708	26.3390004564126	24.35375779704853
24	24.24045336984634	25.463677164156394	26.019245397839647	24.276624068157616
25	24.72166438460368	24.865548455804046	26.143731933668036	24.269055225924234
26	24.540658755514986	25.368324965769055	26.034611288604896	24.056404990111062
27	24.522858664232466	25.007036360870227	25.927886809675947	24.54221816522136
28	24.25045641259699	25.14761144074243	26.2061463563061	24.39578579035448
29	23.940818499923928	25.4024037730108	26.419024798417766	24.237752928647495
30	23.770576601247527	25.71177544500228	26.379583143161415	24.138064810588773
31	24.406587555149855	25.37665449566408	26.074471322075155	24.14228662711091
32	23.844895785790353	25.466225467822913	26.170888483188804	24.51799026319793
33	23.758938080024343	25.233759318423854	26.56089304731477	24.446409554237032
34	24.420546173741062	24.959835691465084	26.083371367716417	24.536246767077436
35	24.164498706830976	25.302183173588926	26.582496576905523	23.95082154267458
36	23.97113190324053	25.426821846949643	26.061273391145594	24.540772858664234
37	24.111212536132665	25.02905826867488	26.34892743039708	24.510801764795374
38	23.79518484710178	24.979765708200212	26.758519701810435	24.466529742887573
39	23.836490187129165	24.78449718545565	26.778792027993305	24.600220599421878
40	23.74638673360718	24.74243115776662	27.17830518788985	24.332876920736346
41	23.207591662863226	24.822607637304124	27.41769359500989	24.55210710482276
42	23.344249201277954	24.984405902936256	27.316293929712458	24.35505096607333
43	23.261676555606268	24.52521679598357	27.412406815761447	24.800699832648714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	365.0
1	418.0
2	471.0
3	1047.0
4	1623.0
5	1623.0
6	2232.5
7	2842.0
8	2723.5
9	2605.0
10	3620.0
11	4635.0
12	4635.0
13	8334.0
14	12033.0
15	16411.0
16	20789.0
17	19609.5
18	18430.0
19	18430.0
20	22737.5
21	27045.0
22	24516.5
23	21988.0
24	25717.5
25	29447.0
26	29447.0
27	33538.0
28	37629.0
29	44506.5
30	51384.0
31	58541.0
32	65698.0
33	65698.0
34	75317.5
35	84937.0
36	95520.5
37	106104.0
38	116168.5
39	126233.0
40	126233.0
41	138145.5
42	150058.0
43	156421.5
44	162785.0
45	170917.0
46	179049.0
47	179049.0
48	185858.0
49	192667.0
50	194067.0
51	195467.0
52	194356.0
53	193245.0
54	193245.0
55	187443.0
56	181641.0
57	173416.0
58	165191.0
59	157492.0
60	149793.0
61	149793.0
62	138541.5
63	127290.0
64	113768.0
65	100246.0
66	87628.5
67	75011.0
68	75011.0
69	64594.5
70	54178.0
71	45555.0
72	36932.0
73	29433.0
74	21934.0
75	21934.0
76	17327.0
77	12720.0
78	9856.0
79	6992.0
80	5648.5
81	4305.0
82	4305.0
83	3202.5
84	2100.0
85	1776.0
86	1452.0
87	1220.0
88	988.0
89	988.0
90	859.5
91	731.0
92	423.0
93	115.0
94	78.0
95	41.0
96	41.0
97	24.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2629200.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.330634650603905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3693673192006	41.79488096299265
2	11.795281202979519	12.580996648744728
3	3.931379972350098	6.289889669343131
4	1.74787568668389	3.7286127864484784
5	0.9933549142869385	2.6488124006109337
6	0.620537516535827	1.9856195748819163
7	0.4311937531508404	1.6097085559037112
8	0.3099460814539808	1.3223696985126854
9	0.22914628578756271	1.099846516399144
>10	1.3794459498333544	13.600633948109808
>50	0.1256302320498943	4.633459349224791
>100	0.06278281921528392	6.092443003663702
>500	0.0032038945833151172	1.1302957988328228
>1k	6.407789166609639E-4	0.5363665162010078
>5k	1.4239531481354754E-4	0.5563767918655931
>10k+	7.119765740677377E-5	0.3896877782649684
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10263	0.3903468735737106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8240	0.31340331659820475	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6413	0.24391449870683096	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4358	0.16575384147269132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.8034383082306405E-5	0.0	0.0	0.0
3	0.0	3.8034383082306405E-5	0.0	0.0	0.0
4	0.0	3.8034383082306405E-5	0.0	0.0	0.0
5	0.0	3.8034383082306405E-5	0.0	0.0	0.0
6	0.0	3.8034383082306405E-5	0.0	0.0	0.0
7	0.0	3.8034383082306405E-5	0.0	0.0	0.0
8	0.0	3.8034383082306405E-5	0.0	0.0	0.0
9	0.0	3.8034383082306405E-5	0.0	0.0	0.0
10	0.0	3.8034383082306405E-5	0.0	0.0	0.0
11	0.0	7.606876616461281E-5	0.0	0.0	0.0
12	0.0	7.606876616461281E-5	0.0	0.0	0.0
13	0.0	7.606876616461281E-5	0.0	3.8034383082306405E-5	0.0
14	0.0	7.606876616461281E-5	0.0	1.9017191541153202E-4	0.0
15	0.0	1.1410314924691921E-4	0.0	4.5641259698767686E-4	0.0
16	0.0	1.1410314924691921E-4	0.0	0.001217100258633805	0.0
17	0.0	1.1410314924691921E-4	0.0	0.0022059942187737714	0.0
18	0.0	1.1410314924691921E-4	0.0	0.002358131751102997	0.0
19	0.0	1.1410314924691921E-4	0.0	0.0028525787311729804	0.0
20	0.0	1.1410314924691921E-4	0.0	0.0034230944774075764	0.0
21	0.0	1.1410314924691921E-4	0.0	0.004373954054465236	0.0
22	0.0	1.1410314924691921E-4	0.0	0.006389776357827476	0.0
23	0.0	1.1410314924691921E-4	0.0	0.008443633044272023	0.0
24	0.0	1.1410314924691921E-4	0.0	0.013273999695724936	0.0
25	0.0	1.1410314924691921E-4	0.0	0.015518028297581013	0.0
26	0.0	1.1410314924691921E-4	0.0	0.019968051118210862	0.0
27	0.0	1.1410314924691921E-4	0.0	0.03153050357523201	0.0
28	0.0	1.1410314924691921E-4	0.0	0.057355849688118056	0.0
29	0.0	1.5213753232922562E-4	0.0	0.09303210101932147	0.0
30	0.0	1.5213753232922562E-4	0.0	0.14464475886201125	0.0
31	0.0	1.5213753232922562E-4	0.0	0.27232618286931387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4440	0.0	16.958334	1
GTACTAG	150	4.6784407E-9	16.033333	1
TACTAGG	230	0.0	15.282609	2
TACGGGT	85	5.3674134E-4	15.235294	4
TATCGGT	115	2.2118742E-5	14.478261	13
GTAAACG	255	1.8189894E-12	13.784313	27
TGCGCGA	100	0.0018335715	12.950001	31
TTAGACT	230	5.7661964E-10	12.869565	4
GTATCAA	6055	0.0	12.496283	2
CTTATAC	1015	0.0	12.394089	37
CTAGACT	225	5.7152647E-9	12.333333	4
GTACGAC	105	0.00264513	12.333333	3
TAGACAG	500	0.0	12.21	5
TCTTATA	1665	0.0	12.111111	37
TATCCCG	125	7.2241324E-4	11.84	5
AAGACGG	460	0.0	11.663044	5
TAAACGC	270	5.802576E-10	11.648148	28
AACGCAG	6620	0.0	11.541541	7
CGCGATA	145	1.9400197E-4	11.4827585	34
GTATTAG	550	0.0	11.436364	1
>>END_MODULE