FastQCFastQC Report
Fri 10 Feb 2017
ERR1630196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685103
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC50160.2976672642562502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.27535408814772744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43610.25879723672677574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33030.19601175714481547No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT28000.1661619497443183No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT21260.12616439469872168No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG19070.1131681564865768No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18310.10865804642208815No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG17630.10462268478544043No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT17160.10183353777187508No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG16890.10023126182791199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.021.5943071
CGTACCC705.1042553E-621.1428573
TCGTACC759.269974E-619.7333342
CCAGGAC14350.018.693383
GTCGTAC500.007036208618.51
TCTAGCG2050.018.04878228
GACAGGC13600.017.9558837
CAGGACA14800.017.754
ACAGGCT13750.017.4909088
GCTGCAT13700.017.28467212
TGCATCA13650.017.07692314
GACGGAC3600.016.9583347
ATACCGT2950.016.9322036
TCCAGGA16400.016.8079282
GCATCAG13900.016.76978515
TTAGACT1554.0199666E-1016.7096794
TAGAGTA1005.88106E-616.6500025
CCGTCGT2600.016.3653859
AAGACGG3850.016.3376645
GTACACG1606.2937033E-1016.18754
Produced by FastQC (version 0.11.5)