##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1685103
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046911079026028	31.0	31.0	34.0	28.0	34.0
2	31.264513801233516	31.0	31.0	34.0	28.0	34.0
3	31.38835252207135	31.0	31.0	34.0	28.0	34.0
4	35.18844723438271	37.0	35.0	37.0	32.0	37.0
5	34.88418571446375	35.0	35.0	37.0	32.0	37.0
6	34.95538432962258	36.0	35.0	37.0	32.0	37.0
7	34.952419525690715	36.0	35.0	37.0	32.0	37.0
8	34.91407765578721	35.0	35.0	37.0	32.0	37.0
9	36.51241200092813	38.0	35.0	39.0	32.0	39.0
10	36.28376128936925	38.0	35.0	39.0	32.0	39.0
11	36.43451587232353	38.0	35.0	39.0	32.0	39.0
12	36.2681474070131	38.0	35.0	39.0	32.0	39.0
13	36.34240696266044	38.0	35.0	39.0	32.0	39.0
14	37.34500977091608	39.0	36.0	40.0	32.0	41.0
15	37.29392327946719	39.0	36.0	40.0	32.0	41.0
16	37.26575764211446	39.0	36.0	40.0	32.0	41.0
17	37.194655756947796	39.0	36.0	40.0	32.0	41.0
18	37.11091784893861	39.0	36.0	40.0	32.0	41.0
19	37.12766222598856	39.0	36.0	40.0	31.0	41.0
20	37.05899105277244	39.0	36.0	40.0	31.0	41.0
21	37.014330874729914	39.0	36.0	40.0	31.0	41.0
22	36.94469062128547	39.0	36.0	40.0	31.0	41.0
23	36.885290691429546	39.0	36.0	40.0	31.0	41.0
24	36.84134204259324	39.0	36.0	40.0	31.0	41.0
25	36.80474487316206	38.0	36.0	40.0	31.0	41.0
26	36.56000078333491	38.0	35.0	40.0	30.0	41.0
27	36.44034934363063	38.0	35.0	40.0	30.0	41.0
28	36.308819104826235	38.0	35.0	40.0	30.0	41.0
29	36.21650724021024	38.0	35.0	40.0	30.0	41.0
30	36.14167857988503	38.0	35.0	40.0	30.0	41.0
31	36.02728616589015	38.0	35.0	40.0	29.0	41.0
32	35.89589360412984	38.0	35.0	40.0	29.0	41.0
33	35.8059768453323	38.0	34.0	40.0	29.0	41.0
34	35.68118684733218	38.0	34.0	40.0	28.0	41.0
35	35.54612329335358	38.0	34.0	40.0	27.0	41.0
36	35.426257029985706	38.0	34.0	40.0	27.0	41.0
37	35.33538127936393	38.0	34.0	40.0	27.0	41.0
38	35.201616162335476	38.0	34.0	40.0	26.0	41.0
39	35.097960184036225	38.0	33.0	40.0	26.0	41.0
40	34.95073357533634	38.0	33.0	40.0	26.0	41.0
41	34.79283580884967	38.0	33.0	40.0	25.0	41.0
42	34.724873791097636	38.0	33.0	40.0	25.0	41.0
43	33.46380013565936	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	2.0
12	2.0
13	6.0
14	13.0
15	34.0
16	85.0
17	165.0
18	293.0
19	538.0
20	1016.0
21	1720.0
22	2918.0
23	4578.0
24	7089.0
25	10415.0
26	14824.0
27	20177.0
28	27313.0
29	35966.0
30	46105.0
31	58819.0
32	74000.0
33	93921.0
34	121023.0
35	160910.0
36	221662.0
37	305899.0
38	349295.0
39	126305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5425051168979	19.80057005417473	12.55495954846677	26.101965280460597
2	19.01741317889767	20.92839428806429	34.399143553836176	25.655048979201865
3	20.330389299645184	23.1772182471932	29.424017404277365	27.068375048884253
4	15.058129977811447	15.734053051949939	34.46359065291558	34.74422631732303
5	14.667530708805337	37.093400225386816	33.702212861765716	14.536856204042126
6	35.14165009497936	35.250248797847966	15.38546901880775	14.222632088364925
7	29.930633320337098	30.506147101987235	20.91723770001003	18.645981877665637
8	27.8551518809236	33.87573341214157	19.345226968321818	18.92388773861301
9	27.18130583115691	13.78651631383957	19.24659798243787	39.78557987256565
10	17.44124839846585	26.610183472464293	32.2693034194349	23.67926470963496
11	36.87525332279392	21.47109108463993	20.84917064416834	20.804484948397814
12	22.305758164337732	25.084104651169692	28.90357444025677	23.70656274423581
13	30.145991075916427	19.005129063327285	24.876461557542772	25.972418303213512
14	22.567047830310667	20.201257727272456	25.14267673845456	32.089017703962305
15	25.529181302270544	27.31862681390989	21.848753458987375	25.30343842483219
16	25.12386483200137	26.046182340189294	23.22469308997729	25.605259737832046
17	23.49031483535428	26.522533043974168	25.329727619023885	24.657424501647675
18	24.07146625458503	24.573928121901154	26.44669198262658	24.907913640887234
19	24.622589835754845	24.873375692761808	25.8411503629155	24.662884108567845
20	24.693089977289223	25.087249859504134	25.738248641180984	24.481411522025656
21	26.348834462937877	24.697540743800232	24.952480649550797	24.001144143711098
22	25.430671003493554	24.926013424698667	25.489539808545832	24.153775763261947
23	24.62241180509441	24.936339203004206	25.450313719695473	24.990935272205913
24	24.888626986006194	25.10000872350236	25.554936404480916	24.45642788601053
25	24.98844284295975	24.713682190346823	25.738604702501867	24.559270264191564
26	25.00179514249277	25.376905744040574	25.758840854238585	23.86245825922807
27	25.3708527015856	24.716115276039506	25.200952107972036	24.71207991440286
28	24.265341643804565	24.546926805067702	26.464554392224095	24.72317715890364
29	24.12730853840982	24.714572343649024	26.53440175467019	24.62371736327097
30	24.052476317471395	25.393581282568483	26.19727102734966	24.356671372610457
31	24.52164645128517	25.29981846806991	25.633507269288586	24.545027811356338
32	23.907203298552076	24.991350677080273	25.82394073240627	25.277505291961383
33	24.213416034509464	25.017758558378922	25.996808503693842	24.772016903417775
34	24.978176408207688	24.921622001741138	25.76780173081408	24.332399859237093
35	25.166058098525728	24.89402724937289	26.32598719484803	23.613927457253357
36	24.475358479570687	25.63249842887942	25.065352088269975	24.82679100327992
37	24.412394969328286	24.959720563075372	25.95325033543944	24.674634132156907
38	24.6821114198954	24.74068350718027	26.23875217123226	24.33845290169206
39	23.9820355194905	24.768634320869406	26.25228250142573	24.997047658214363
40	24.260890877293555	24.39536336947949	27.16617322501948	24.177572528207474
41	23.22510849485165	24.48348854639746	27.50021808755904	24.79118487119185
42	23.85076757919249	24.824417261140713	26.813553830240643	24.511261329426155
43	23.69582156105591	23.361836042069832	27.623830709458115	25.31851168741614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	209.0
2	174.0
3	345.5
4	517.0
5	517.0
6	755.0
7	993.0
8	1008.0
9	1023.0
10	1366.0
11	1709.0
12	1709.0
13	3228.0
14	4747.0
15	7585.0
16	10423.0
17	10124.5
18	9826.0
19	9826.0
20	11869.5
21	13913.0
22	14138.0
23	14363.0
24	16897.5
25	19432.0
26	19432.0
27	23287.5
28	27143.0
29	32073.5
30	37004.0
31	42329.5
32	47655.0
33	47655.0
34	55054.0
35	62453.0
36	69826.0
37	77199.0
38	82600.0
39	88001.0
40	88001.0
41	92574.0
42	97147.0
43	99218.0
44	101289.0
45	103994.5
46	106700.0
47	106700.0
48	108216.5
49	109733.0
50	111962.0
51	114191.0
52	118485.0
53	122779.0
54	122779.0
55	123039.5
56	123300.0
57	117085.0
58	110870.0
59	101143.0
60	91416.0
61	91416.0
62	85271.5
63	79127.0
64	68205.5
65	57284.0
66	48611.5
67	39939.0
68	39939.0
69	34518.0
70	29097.0
71	26123.5
72	23150.0
73	23723.0
74	24296.0
75	24296.0
76	22067.0
77	19838.0
78	15361.0
79	10884.0
80	7188.5
81	3493.0
82	3493.0
83	2434.0
84	1375.0
85	1171.0
86	967.0
87	812.5
88	658.0
89	658.0
90	619.0
91	580.0
92	347.5
93	115.0
94	77.5
95	40.0
96	40.0
97	25.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1685103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.867579139228454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.35004790899161	49.9686823104974
2	8.582233772209172	9.932661840093468
3	2.0735221099430814	3.599691143822136
4	0.850952698084346	1.9697029040054346
5	0.4553348943490397	1.3174564016797639
6	0.27693107872178646	0.9615198668426929
7	0.2000575894268834	0.8103793871994414
8	0.14531540283712718	0.6727240459061057
9	0.10853510317855278	0.5652597305312307
>10	0.753223836351064	8.77352844663582
>50	0.10343842347911134	4.224989529816093
>100	0.0884492759812118	10.409819565626066
>500	0.008968429835254593	3.5132649462742185
>1k	0.002886391211346306	2.9811003043570388
>5k	1.0308540040522522E-4	0.2992195767129889
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5016	0.2976672642562502	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4640	0.27535408814772744	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4361	0.25879723672677574	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3303	0.19601175714481547	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2800	0.1661619497443183	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2126	0.12616439469872168	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1907	0.1131681564865768	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1831	0.10865804642208815	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1763	0.10462268478544043	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1716	0.10183353777187508	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1689	0.10023126182791199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.934355348011368E-5	0.0	0.0	0.0
6	0.0	5.934355348011368E-5	0.0	0.0	0.0
7	0.0	5.934355348011368E-5	0.0	5.934355348011368E-5	0.0
8	0.0	5.934355348011368E-5	0.0	5.934355348011368E-5	5.934355348011368E-5
9	0.0	5.934355348011368E-5	0.0	5.934355348011368E-5	5.934355348011368E-5
10	5.934355348011368E-5	5.934355348011368E-5	0.0	1.7803066044034103E-4	5.934355348011368E-5
11	5.934355348011368E-5	5.934355348011368E-5	0.0	1.7803066044034103E-4	5.934355348011368E-5
12	5.934355348011368E-5	5.934355348011368E-5	0.0	1.7803066044034103E-4	5.934355348011368E-5
13	5.934355348011368E-5	5.934355348011368E-5	0.0	1.7803066044034103E-4	5.934355348011368E-5
14	5.934355348011368E-5	5.934355348011368E-5	0.0	3.5606132088068207E-4	5.934355348011368E-5
15	5.934355348011368E-5	5.934355348011368E-5	0.0	3.5606132088068207E-4	5.934355348011368E-5
16	5.934355348011368E-5	5.934355348011368E-5	0.0	7.714661952414778E-4	5.934355348011368E-5
17	5.934355348011368E-5	5.934355348011368E-5	0.0	0.001305558176562501	5.934355348011368E-5
18	5.934355348011368E-5	5.934355348011368E-5	0.0	0.0013649017300426146	5.934355348011368E-5
19	5.934355348011368E-5	5.934355348011368E-5	0.0	0.0016022759439630694	5.934355348011368E-5
20	5.934355348011368E-5	5.934355348011368E-5	0.0	0.0017209630509232967	5.934355348011368E-5
21	5.934355348011368E-5	5.934355348011368E-5	0.0	0.001958337264843751	5.934355348011368E-5
22	5.934355348011368E-5	5.934355348011368E-5	0.0	0.002611116353125002	5.934355348011368E-5
23	5.934355348011368E-5	5.934355348011368E-5	0.0	0.0040353616366477305	5.934355348011368E-5
24	5.934355348011368E-5	5.934355348011368E-5	0.0	0.006053042454971595	5.934355348011368E-5
25	5.934355348011368E-5	5.934355348011368E-5	0.0	0.007121226417613641	5.934355348011368E-5
26	5.934355348011368E-5	5.934355348011368E-5	0.0	0.009376281449857962	5.934355348011368E-5
27	5.934355348011368E-5	5.934355348011368E-5	0.0	0.017031599848792624	5.934355348011368E-5
28	5.934355348011368E-5	5.934355348011368E-5	0.0	0.040472303473437526	5.934355348011368E-5
29	5.934355348011368E-5	5.934355348011368E-5	0.0	0.0745948467245029	5.934355348011368E-5
30	5.934355348011368E-5	5.934355348011368E-5	0.0	0.12557095916392055	5.934355348011368E-5
31	5.934355348011368E-5	5.934355348011368E-5	0.0	0.26396012587954565	5.934355348011368E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1405	0.0	21.594307	1
CGTACCC	70	5.1042553E-6	21.142857	3
TCGTACC	75	9.269974E-6	19.733334	2
CCAGGAC	1435	0.0	18.69338	3
GTCGTAC	50	0.0070362086	18.5	1
TCTAGCG	205	0.0	18.048782	28
GACAGGC	1360	0.0	17.955883	7
CAGGACA	1480	0.0	17.75	4
ACAGGCT	1375	0.0	17.490908	8
GCTGCAT	1370	0.0	17.284672	12
TGCATCA	1365	0.0	17.076923	14
GACGGAC	360	0.0	16.958334	7
ATACCGT	295	0.0	16.932203	6
TCCAGGA	1640	0.0	16.807928	2
GCATCAG	1390	0.0	16.769785	15
TTAGACT	155	4.0199666E-10	16.709679	4
TAGAGTA	100	5.88106E-6	16.650002	5
CCGTCGT	260	0.0	16.365385	9
AAGACGG	385	0.0	16.337664	5
GTACACG	160	6.2937033E-10	16.1875	4
>>END_MODULE