FastQCFastQC Report
Fri 10 Feb 2017
ERR1630195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959299
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.22671373792361454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40420.20629827300478387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34350.17531780499045832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.021.0945131
CTTATAC10000.016.27999937
TCTTATA20000.016.002537
TCTACGG1201.9370964E-615.4166672
TCGAACG855.3665467E-415.2352943
CCGTTGC750.004105778414.832
TCTGTCG1252.9611383E-614.7999998
CGAACGT908.2776835E-414.3888884
GTGTACG908.2776835E-414.3888881
AGAACCG1702.4392648E-814.1470595
TAGAACA7450.013.6577184
CCGAATT1907.1340764E-913.63157914
TACGTTA1254.847788E-513.3219
CGAATTA1951.0270014E-813.28205215
CTAGTAC850.00940782213.0588243
GTATCAA26400.012.8939392
AGCGTTG1307.002723E-512.80769329
TAGTGTC1451.3730301E-512.758627
GTAGAAC6100.012.7377053
TAATACA4400.012.6136364
Produced by FastQC (version 0.11.5)