FastQCFastQC Report
Fri 10 Feb 2017
ERR1630194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277759
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78472.825110977502079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52001.872126555755169No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44991.6197494950658666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.927422693774099No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10200.3672248243981293No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8140.293059810843213No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8130.29269978650556777No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.28765944577853464No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.28729942144088944No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.2203348946388776No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.21241435921068264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.14112954035692812No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3750.1350091266169593No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.13392905360402363No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3560.12816866420170003No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3470.124928445162893No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.10908737430650312No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3010.10836732563121268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2840.10224691189124385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG250.00548925829.618
TAGAGGT250.00548925829.619
TGCGCGA250.00548925829.612
GCTAGAG250.00548925829.617
CATCGGA250.00548925829.619
CAATGCG250.00548925829.619
AGAGGTG250.00548925829.620
GTAAACA250.00548925829.636
ACTGTGC601.3313147E-624.6666688
CGCCAGC551.8959323E-523.54545434
CGTGCGC400.00192752223.12510
ACGTGCG400.00192752223.1259
TCCTCGC502.6949594E-422.221
ATACTGG502.6949594E-422.26
GATATGC502.6949594E-422.234
AGACACT502.6949594E-422.226
CTGTGCG502.6949594E-422.29
GCCCCGT1059.731593E-1021.14285714
AATGCAC1059.731593E-1021.14285728
CCCCGTA1059.731593E-1021.14285715
Produced by FastQC (version 0.11.5)