##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630194.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277759
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55819613405866	31.0	31.0	34.0	30.0	34.0
2	31.696269067788982	31.0	31.0	34.0	30.0	34.0
3	31.74887942424908	33.0	31.0	34.0	30.0	34.0
4	35.489384682404534	37.0	35.0	37.0	33.0	37.0
5	35.46649793526042	37.0	35.0	37.0	33.0	37.0
6	35.56335528281712	37.0	35.0	37.0	33.0	37.0
7	35.52785328288192	37.0	35.0	37.0	33.0	37.0
8	35.59099435121814	37.0	35.0	37.0	33.0	37.0
9	37.23091240967889	39.0	37.0	39.0	34.0	39.0
10	37.10313617200523	39.0	37.0	39.0	33.0	39.0
11	37.20666477053849	39.0	37.0	39.0	34.0	39.0
12	37.13536195046785	39.0	37.0	39.0	33.0	39.0
13	37.184940902004975	39.0	37.0	39.0	33.0	39.0
14	38.194117922371554	40.0	37.0	41.0	33.0	41.0
15	38.18413444748865	40.0	37.0	41.0	33.0	41.0
16	38.09445958546798	40.0	37.0	41.0	33.0	41.0
17	38.20033914292606	40.0	38.0	41.0	33.0	41.0
18	38.229418308677666	40.0	38.0	41.0	33.0	41.0
19	38.318074301822804	40.0	38.0	41.0	34.0	41.0
20	38.320472063911524	40.0	38.0	41.0	34.0	41.0
21	38.30645631644699	40.0	38.0	41.0	34.0	41.0
22	38.24530258245457	40.0	38.0	41.0	34.0	41.0
23	38.1625077855263	40.0	37.0	41.0	33.0	41.0
24	38.10347459488261	40.0	37.0	41.0	33.0	41.0
25	38.06736055357342	40.0	37.0	41.0	33.0	41.0
26	37.80378673598335	40.0	37.0	41.0	33.0	41.0
27	37.59414096392916	40.0	36.0	41.0	33.0	41.0
28	37.46571308220436	39.0	36.0	41.0	32.0	41.0
29	37.309577727454375	39.0	36.0	41.0	32.0	41.0
30	37.076145147411964	39.0	35.0	41.0	32.0	41.0
31	36.83132859781321	39.0	35.0	40.0	31.0	41.0
32	36.61679729549718	38.0	35.0	40.0	31.0	41.0
33	36.41762823166846	38.0	35.0	40.0	31.0	41.0
34	36.28478285132075	38.0	35.0	40.0	30.0	41.0
35	36.04942774131531	38.0	35.0	40.0	30.0	41.0
36	35.82303363707386	38.0	35.0	40.0	30.0	41.0
37	35.6035988032791	38.0	35.0	40.0	28.0	41.0
38	35.35257183385597	38.0	35.0	40.0	27.0	41.0
39	35.09018249633675	38.0	34.0	40.0	25.0	41.0
40	34.785781918857715	38.0	34.0	40.0	24.0	41.0
41	34.46095716070406	38.0	33.0	40.0	22.0	41.0
42	34.16201455218373	38.0	33.0	40.0	21.0	41.0
43	33.00231855673444	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	3.0
12	3.0
13	3.0
14	0.0
15	3.0
16	1.0
17	4.0
18	14.0
19	36.0
20	62.0
21	132.0
22	274.0
23	470.0
24	827.0
25	1266.0
26	1936.0
27	2808.0
28	3792.0
29	5086.0
30	6368.0
31	7996.0
32	9803.0
33	12525.0
34	16030.0
35	20169.0
36	28166.0
37	44955.0
38	59361.0
39	55658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.879913882178435	20.49510546912971	14.436975939573514	20.18800470911834
2	17.86692780431957	26.09564406553883	33.43438016409909	22.603047966042507
3	19.918346480222063	28.46064393953031	31.55541314592866	20.065596434318962
4	13.560316677407394	17.899690019045288	35.14377571923862	33.3962175843087
5	12.079896601010228	41.73150104947094	32.40759075313491	13.781011596383916
6	28.14922288746719	37.41840948448115	18.068181409063254	16.3641862189884
7	24.67390795617784	34.007178885292646	23.10708203874582	18.211831119783696
8	29.732249900093244	32.18257554210665	20.22616728890873	17.85900726889138
9	27.612066575700517	13.016319903225456	22.395673947558855	36.97593957351517
10	18.73494648238221	30.14519781537232	29.870859270086658	21.248996432158815
11	30.933651114815362	26.697604758081646	20.55270936315295	21.816034763950043
12	19.555081923538033	31.97196130458419	28.93119574883262	19.541761023045158
13	30.514942810133967	21.30588027750676	26.776090063688308	21.40308684867097
14	21.981645959266846	23.252891895492137	30.044030976494014	24.721431168747007
15	25.046173121303	28.713741048894903	25.91455182370328	20.32553400609881
16	20.002232150893402	27.425573968800293	29.06404472942371	23.5081491508826
17	18.846194002714583	29.814695473414005	29.939623918576896	21.39948660529452
18	17.964854424159075	26.570516166892883	32.92206553162994	22.542563877318106
19	18.944120622554085	27.836361738053494	35.006966470933435	18.212551168458987
20	19.449234768270333	27.29524515857272	34.819393791020275	18.436126282136673
21	20.69203878182165	26.83441400638683	33.417819044567416	19.055728167224103
22	21.002379760871833	27.314686472805562	32.74097328979439	18.941960476528212
23	19.744094700801774	28.26875096756541	32.65528749743483	19.331866834197992
24	18.388963093905147	29.237216435831066	32.72621229195094	19.647608178312854
25	19.221339362540906	28.599253309523725	33.198204198603825	18.981203129331544
26	18.972922569565704	29.114808161031686	32.83961995830918	19.07264931109343
27	18.37528216907463	29.601201041190382	32.92134548295465	19.10217130678034
28	18.22083172822483	30.310088962013833	33.907812168102566	17.561267141658774
29	18.842953783675775	29.82693630089394	33.399817827685155	17.93029208774513
30	18.14450656864404	31.006016006682053	33.125839306737134	17.723638117936773
31	18.643140276282676	30.150238156099352	33.094157165024356	18.112464402593613
32	17.249846089595657	30.996655373903277	33.07435582645387	18.6791427100472
33	17.3520930014869	30.85696593089693	33.43149996939793	18.35944109821824
34	17.735518921079066	30.3424911524019	33.09163699466084	18.830352931858194
35	17.88816924024064	30.940491577230624	32.940066748512194	18.23127243401654
36	17.66963446728999	30.88900809694735	32.02956519860742	19.411792237155232
37	17.703836779366284	30.388574267620488	32.62828567211143	19.27930328090179
38	17.39205570296552	30.51170259109516	32.62216525837147	19.474076447567857
39	17.31213030000828	29.688326930900526	33.98881764407274	19.010725125018453
40	17.333371735929347	29.766452212169543	33.92977365269893	18.970402399202186
41	17.09035530801882	29.503274421350884	33.92725348233541	19.47911678829489
42	16.23169726273496	29.883100097566594	33.83292710587236	20.052275533826087
43	16.435111013504514	29.281139405023783	33.92509333630954	20.358656245162173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	406.0
1	306.5
2	207.0
3	493.0
4	779.0
5	779.0
6	1121.5
7	1464.0
8	1383.5
9	1303.0
10	1857.0
11	2411.0
12	2411.0
13	4810.0
14	7209.0
15	11510.5
16	15812.0
17	12877.0
18	9942.0
19	9942.0
20	10253.5
21	10565.0
22	7396.5
23	4228.0
24	4416.0
25	4604.0
26	4604.0
27	4914.5
28	5225.0
29	5677.0
30	6129.0
31	6734.5
32	7340.0
33	7340.0
34	8694.0
35	10048.0
36	11021.0
37	11994.0
38	13170.5
39	14347.0
40	14347.0
41	16167.0
42	17987.0
43	19602.5
44	21218.0
45	21969.0
46	22720.0
47	22720.0
48	23935.0
49	25150.0
50	22342.0
51	19534.0
52	17760.0
53	15986.0
54	15986.0
55	14543.0
56	13100.0
57	11237.0
58	9374.0
59	7986.0
60	6598.0
61	6598.0
62	5502.0
63	4406.0
64	3600.0
65	2794.0
66	2311.5
67	1829.0
68	1829.0
69	1489.0
70	1149.0
71	920.5
72	692.0
73	557.0
74	422.0
75	422.0
76	358.0
77	294.0
78	260.0
79	226.0
80	166.5
81	107.0
82	107.0
83	84.5
84	62.0
85	56.5
86	51.0
87	41.5
88	32.0
89	32.0
90	21.0
91	10.0
92	7.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	277759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.74321624141792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86120064464141	25.32807217767921
2	11.942989524576953	8.537617142918862
3	4.949637389202256	5.3074787855659045
4	2.794117647058824	3.9948300505114145
5	1.7395245769540693	3.108810155566516
6	1.2902900886381952	2.7671470591411977
7	0.911563255439162	2.280754178982499
8	0.750402900886382	2.14574505236554
9	0.5902497985495568	1.8987683567409157
>10	3.87187751813054	26.301938011009547
>50	0.22159548751007255	5.2909176660342245
>100	0.06547139403706688	3.8328190985710635
>500	0.0060435132957292505	1.593467718417765
>1k	0.0030217566478646252	2.9143970132380947
>5k	0.0020145044319097504	4.697237533257248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7847	2.825110977502079	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5200	1.872126555755169	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4499	1.6197494950658666	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2576	0.927422693774099	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1020	0.3672248243981293	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	814	0.293059810843213	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	813	0.29269978650556777	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	799	0.28765944577853464	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	798	0.28729942144088944	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	612	0.2203348946388776	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.21241435921068264	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.14112954035692812	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	375	0.1350091266169593	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.13392905360402363	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	356	0.12816866420170003	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	347	0.124928445162893	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	303	0.10908737430650312	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	301	0.10836732563121268	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	284	0.10224691189124385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.6002433764522484E-4	0.0
8	0.0	0.0	0.0	3.6002433764522484E-4	0.0
9	0.0	0.0	0.0	3.6002433764522484E-4	0.0
10	0.0	0.0	0.0	3.6002433764522484E-4	3.6002433764522484E-4
11	0.0	0.0	0.0	3.6002433764522484E-4	3.6002433764522484E-4
12	0.0	0.0	0.0	3.6002433764522484E-4	3.6002433764522484E-4
13	0.0	0.0	0.0	3.6002433764522484E-4	3.6002433764522484E-4
14	0.0	0.0	0.0	7.200486752904497E-4	7.200486752904497E-4
15	0.0	0.0	0.0	7.200486752904497E-4	7.200486752904497E-4
16	0.0	0.0	0.0	0.002160146025871349	7.200486752904497E-4
17	0.0	0.0	0.0	0.0025201703635165736	7.200486752904497E-4
18	0.0	0.0	0.0	0.0025201703635165736	7.200486752904497E-4
19	0.0	0.0	0.0	0.0032402190388070235	7.200486752904497E-4
20	0.0	0.0	0.0	0.003600243376452248	7.200486752904497E-4
21	0.0	0.0	0.0	0.005040340727033147	7.200486752904497E-4
22	0.0	0.0	0.0	0.007560511090549721	0.0010800730129356744
23	0.0	0.0	0.0	0.011160754467001969	0.0010800730129356744
24	0.0	0.0	0.0	0.01728116820697079	0.0014400973505808994
25	0.0	0.0	0.0	0.019801338570487364	0.0014400973505808994
26	0.0	0.0	0.0	0.025561727972810963	0.0014400973505808994
27	0.0	0.0	0.0	0.04608311521858878	0.0014400973505808994
28	0.0	0.0	0.0	0.09036610874895143	0.0014400973505808994
29	0.0	0.0	0.0	0.1641710979662225	0.0014400973505808994
30	0.0	0.0	0.0	0.3132211737513456	0.0014400973505808994
31	0.0	0.0	0.0	0.6718054140459895	0.0014400973505808994
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGG	25	0.005489258	29.6	18
TAGAGGT	25	0.005489258	29.6	19
TGCGCGA	25	0.005489258	29.6	12
GCTAGAG	25	0.005489258	29.6	17
CATCGGA	25	0.005489258	29.6	19
CAATGCG	25	0.005489258	29.6	19
AGAGGTG	25	0.005489258	29.6	20
GTAAACA	25	0.005489258	29.6	36
ACTGTGC	60	1.3313147E-6	24.666668	8
CGCCAGC	55	1.8959323E-5	23.545454	34
CGTGCGC	40	0.001927522	23.125	10
ACGTGCG	40	0.001927522	23.125	9
TCCTCGC	50	2.6949594E-4	22.2	21
ATACTGG	50	2.6949594E-4	22.2	6
GATATGC	50	2.6949594E-4	22.2	34
AGACACT	50	2.6949594E-4	22.2	26
CTGTGCG	50	2.6949594E-4	22.2	9
GCCCCGT	105	9.731593E-10	21.142857	14
AATGCAC	105	9.731593E-10	21.142857	28
CCCCGTA	105	9.731593E-10	21.142857	15
>>END_MODULE