FastQCFastQC Report
Fri 10 Feb 2017
ERR1630193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924892
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC72900.3787225465117004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71240.37009868605615276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63720.33103155917319No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT49660.2579885001340335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49520.2572611866016379No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.17923083476891172No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT33510.17408768907554295No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG31700.16468456412100002No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG31100.16156750612501897No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG27610.14343661878172903No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG27410.14239759944973537No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT27040.14047541368554703No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC24440.12696816236962905No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG23800.12364330050724924No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG23650.12286403600825398No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT23150.12026648767826974No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT22970.11933137027947541No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG22450.11662992001629181No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT22230.11548699875109877No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC22110.11486358715190254No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT21990.11424017555270634No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG21460.11148677432292306No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG20700.10753850086134703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG1103.855712E-818.530
TTTAGCG1002.877423E-718.49999826
GGTATCA26700.017.3220981
GGACCGT650.001580457617.0769236
CGCGGAA1201.04224455E-716.95833433
TCGCGGA1251.6593003E-716.27999932
TAATACG1151.2431847E-616.0869564
GACAGGC22000.015.3886367
TGCATCA22200.015.014
CGCGCTT1508.1094186E-814.812
AACACCG750.004105743514.85
AGGCCAT22800.014.68640325
GCATCAG22400.014.61830415
TCGCGCT1651.646913E-814.57575911
CCAGGAC25800.014.4844963
ATCAAGC22700.014.4251130
CAGGACA25050.014.4011974
CGTAGCC1803.3342076E-914.3888883
CGATCGG908.277593E-414.38888836
GGCCATC23650.014.23678626
Produced by FastQC (version 0.11.5)