FastQCFastQC Report
Fri 10 Feb 2017
ERR1630192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368898
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143713.8956567940189433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109132.9582703077815546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88262.392531268806011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53591.4527050837900992No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18350.4974274731768673No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.4667956996242864No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15460.4190860346220364No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.3551117110962922No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12920.3502323135392439No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12880.34914800297101095No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.2865290676555579No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.21469349251012473No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.18107986489490321No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.17647154497991316No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6230.16888137100228248No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA5970.16183335230876827No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5590.15153240191055523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5590.15153240191055523No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5540.15017701370026404No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.14312899500674983No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5160.13987606330205096No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.1371652868814686No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4640.1257800259150226No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4400.11927416250562486No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4320.11710554136915895No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.10870213446535357No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.10463596983448No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3780.10246734869801409No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3720.10084088284566466No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3690.10002764991948994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC352.383945E-531.71428532
ACCTAAA303.5947823E-430.8333321
AACGTAT250.00549156529.615
GACGTTA250.00549156529.67
GAACGTA250.00549156529.614
ACGTATT250.00549156529.616
AGGACTC405.9293656E-527.755
TGACGAT405.9293656E-527.7523
CATCCAG405.9293656E-527.7516
ACTTCTA358.8579336E-426.4285714
TAGTACT358.8579336E-426.4285733
CGCATTG451.3208333E-424.66666814
GTAGGAC451.3208333E-424.6666683
AGACCGT451.3208333E-424.66666821
TGCCTAG451.3208333E-424.66666829
GCCTAGT451.3208333E-424.66666830
TAGGACT451.3208333E-424.6666684
ATGCCTA451.3208333E-424.66666828
GACTCGT451.3208333E-424.6666687
TGTAGGA551.8981207E-523.5454542
Produced by FastQC (version 0.11.5)