FastQCFastQC Report
Fri 10 Feb 2017
ERR1630191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159694
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100420.4649732786218788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90460.4188556341778048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69900.32365696251413395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50650.23452396496911138No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT27570.12765697362681935No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG27530.1274717622033492No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23820.11029340267649029No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA23630.10941364841500693No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23000.10649656849535166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC752.0683269E-417.2666661
GGTATCA48350.016.3764231
ATCGGTC803.3842877E-416.187514
AAACCCG700.002593483515.8571425
CGTCGTA1752.240995E-914.810
TATCCCG750.004105959614.85
TCTAGCG1904.5656634E-1014.60526428
CTAGCGG2009.840733E-1013.87499929
GAGTCCG1356.5729E-613.7037037
TAGACAG2304.0017767E-1113.6739135
GTCGCGT2601.8189894E-1213.5192318
CGGCCTT7600.013.38815824
GCGTTAT2355.638867E-1113.3829791
TCGCCAT5950.013.36974713
TTCCTCG7900.013.34810119
CTTATAC12500.013.3237
TCGCGTA2651.8189894E-1213.2641529
TCTCGCC7750.013.12903312
TCTTATA20750.013.10602437
CTATCGG1000.001833387712.94999912
Produced by FastQC (version 0.11.5)