##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2159694
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83478816906469	33.0	31.0	34.0	30.0	34.0
2	32.04608384335929	33.0	31.0	34.0	30.0	34.0
3	32.160131481589524	34.0	31.0	34.0	30.0	34.0
4	35.79771439842867	37.0	35.0	37.0	35.0	37.0
5	35.726805278895995	37.0	35.0	37.0	35.0	37.0
6	35.79359622242781	37.0	35.0	37.0	35.0	37.0
7	35.774416653470354	37.0	35.0	37.0	35.0	37.0
8	35.78710132083527	37.0	35.0	37.0	35.0	37.0
9	37.51818266847063	39.0	37.0	39.0	35.0	39.0
10	37.42227602614074	39.0	37.0	39.0	34.0	39.0
11	37.48480618087562	39.0	37.0	39.0	35.0	39.0
12	37.42310623634645	39.0	37.0	39.0	34.0	39.0
13	37.42998591467124	39.0	37.0	39.0	34.0	39.0
14	38.659424900008986	40.0	38.0	41.0	34.0	41.0
15	38.670091688915186	40.0	38.0	41.0	34.0	41.0
16	38.615543220474756	40.0	38.0	41.0	34.0	41.0
17	38.61159775412628	40.0	38.0	41.0	34.0	41.0
18	38.57321083449785	40.0	38.0	41.0	34.0	41.0
19	38.625233019122156	40.0	38.0	41.0	34.0	41.0
20	38.612284888507354	40.0	38.0	41.0	34.0	41.0
21	38.55583661389067	40.0	38.0	41.0	34.0	41.0
22	38.522634225033734	40.0	38.0	41.0	34.0	41.0
23	38.46485844753933	40.0	38.0	41.0	34.0	41.0
24	38.427757358218344	40.0	38.0	41.0	34.0	41.0
25	38.40455684925735	40.0	38.0	41.0	34.0	41.0
26	38.24069752474193	40.0	38.0	41.0	34.0	41.0
27	38.11799958697853	40.0	38.0	41.0	33.0	41.0
28	38.05471099146453	40.0	37.0	41.0	33.0	41.0
29	37.99702781968186	40.0	37.0	41.0	33.0	41.0
30	37.92076099669676	40.0	37.0	41.0	33.0	41.0
31	37.84865402228279	40.0	37.0	41.0	33.0	41.0
32	37.74248203680707	40.0	37.0	41.0	33.0	41.0
33	37.649238271718126	40.0	37.0	41.0	33.0	41.0
34	37.58183705654597	40.0	37.0	41.0	33.0	41.0
35	37.45369297687543	40.0	37.0	41.0	32.0	41.0
36	37.35821556201943	40.0	36.0	41.0	32.0	41.0
37	37.291131984438536	40.0	36.0	41.0	31.0	41.0
38	37.17163311098702	40.0	36.0	41.0	31.0	41.0
39	37.07224495692445	40.0	36.0	41.0	31.0	41.0
40	36.958427443887885	39.0	36.0	41.0	31.0	41.0
41	36.82658006180505	39.0	36.0	41.0	30.0	41.0
42	36.77251823637978	39.0	35.0	41.0	30.0	41.0
43	35.61619423862825	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	10.0
11	8.0
12	5.0
13	6.0
14	7.0
15	11.0
16	17.0
17	48.0
18	91.0
19	178.0
20	339.0
21	656.0
22	1268.0
23	2120.0
24	3477.0
25	5645.0
26	8654.0
27	12836.0
28	18190.0
29	25068.0
30	33368.0
31	44027.0
32	56422.0
33	72351.0
34	94864.0
35	127219.0
36	177486.0
37	270673.0
38	493845.0
39	710799.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.741746747455885	20.54082661710409	13.330962627112916	24.386464008327106
2	17.691811895574094	22.378355452207582	36.144981650178224	23.784851002040103
3	19.8343376422771	24.583899385746314	30.848860996048515	24.73290197592807
4	14.798439038122993	16.486455951630184	34.97754774518983	33.73755726505699
5	13.747040089938666	37.79498391901816	34.37130445331607	14.086671537727105
6	32.6632847060741	35.47261788012561	16.645228444399994	15.218868969400296
7	26.9136275787218	31.942858571630982	22.535692556445497	18.607821293201724
8	25.547091393502967	35.02561010958034	21.122205275376974	18.305093221539718
9	25.83791963120701	15.266792425223203	21.345709160649612	37.54957878292017
10	16.837940930520713	27.905619962828066	33.69708856902876	21.55935053762246
11	33.25281266697967	23.341223339973162	22.44396659897189	20.961997394075272
12	19.92296130840758	27.459214129409077	30.24428460698599	22.37353995519736
13	28.521447945866406	21.14378240621125	26.715127235617636	23.619642412304707
14	21.927828664616374	22.74076790508285	26.399063941465784	28.93233948883499
15	24.358543386238978	28.933635968799283	24.08021691961917	22.62760372534257
16	23.442858108602422	27.903906757160968	25.577003038393404	23.076232095843206
17	22.23796519321719	28.059901078578726	26.856536157437116	22.84559757076697
18	21.365573085816784	26.514589566855307	28.750184053852074	23.369653293475835
19	22.55111140744939	27.012669387422477	29.031057177544596	21.40516202758354
20	23.054979085000006	26.15902993664843	28.94650816273046	21.839482815621103
21	22.91444991744201	27.092217693802915	27.656834718251755	22.336497670503324
22	22.830780656889356	26.664842334145483	27.949839190181574	22.554537818783587
23	22.174252463543446	27.075131939987795	28.085691769296943	22.664923827171812
24	23.179024435869156	27.255805683582953	27.923446562337073	21.641723318210822
25	22.98737691543339	26.465230722500504	28.0322582736258	22.515134088440306
26	22.657469067377136	27.064250768858923	27.889645477553763	22.388634686210178
27	22.801563554836935	26.62548490665807	27.614282393709477	22.95866914479551
28	22.02219388487443	26.978636788359832	28.47727502136877	22.52189430539697
29	22.189300891700398	27.379202794469958	28.23645386800167	22.195042445827973
30	21.78901270272548	28.18700241793513	28.035730987815867	21.98825389152352
31	22.52624677384852	27.36119098353748	28.18589114939431	21.92667109321969
32	21.596161308037157	27.375452263144684	28.102360797409265	22.926025631408894
33	21.814525576308498	27.101848687823367	28.443196119450253	22.640429616417883
34	22.194764628692766	26.913303458730724	28.227887839666177	22.66404407291033
35	22.400580823024	26.875288814063474	28.296323460638405	22.42780690227412
36	21.86444005493371	27.34841139531804	28.20172672610101	22.585421823647238
37	22.702429140424524	26.13999946288687	28.04753821606209	23.110033180626516
38	21.963898589337195	26.66113810567608	28.911364295126994	22.46359900985973
39	22.07271030062592	26.247653602778914	29.036382005969365	22.643254090625803
40	21.753220595139865	25.780967118489933	29.78398791680673	22.681824369563465
41	21.04381454039322	26.543482548916653	29.83871789244217	22.573985018247956
42	21.682516134230127	25.778790884264158	29.57405076830329	22.964642213202428
43	20.862724071095258	25.933766542852833	29.620770349873638	23.582739036178275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1133.0
1	987.5
2	842.0
3	1751.5
4	2661.0
5	2661.0
6	3384.5
7	4108.0
8	3945.5
9	3783.0
10	5423.0
11	7063.0
12	7063.0
13	11364.0
14	15665.0
15	21640.5
16	27616.0
17	26296.0
18	24976.0
19	24976.0
20	28204.5
21	31433.0
22	31973.0
23	32513.0
24	36230.0
25	39947.0
26	39947.0
27	44276.0
28	48605.0
29	61200.5
30	73796.0
31	77433.5
32	81071.0
33	81071.0
34	86919.5
35	92768.0
36	98548.5
37	104329.0
38	115385.5
39	126442.0
40	126442.0
41	134899.5
42	143357.0
43	136787.5
44	130218.0
45	136673.0
46	143128.0
47	143128.0
48	146754.0
49	150380.0
50	152314.5
51	154249.0
52	157920.0
53	161591.0
54	161591.0
55	143826.5
56	126062.0
57	117972.0
58	109882.0
59	98587.5
60	87293.0
61	87293.0
62	79177.5
63	71062.0
64	62364.5
65	53667.0
66	46149.5
67	38632.0
68	38632.0
69	32599.5
70	26567.0
71	22901.5
72	19236.0
73	15218.5
74	11201.0
75	11201.0
76	8503.5
77	5806.0
78	4700.5
79	3595.0
80	2850.0
81	2105.0
82	2105.0
83	1573.5
84	1042.0
85	911.5
86	781.0
87	675.5
88	570.0
89	570.0
90	482.0
91	394.0
92	240.5
93	87.0
94	56.0
95	25.0
96	25.0
97	16.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2159694.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.26265035641353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.15359512260285	43.458272677713666
2	10.40751872721222	10.878490246362365
3	2.7504977291093735	4.312449033676579
4	1.098302041521222	2.2960070232703518
5	0.5802909355443556	1.516377113467538
6	0.35704955379837267	1.119621359404669
7	0.2606894753737321	0.9537026032137966
8	0.1732520427975723	0.7243688749011132
9	0.13978093571565783	0.6574789952819767
>10	0.849297244803488	8.533787746534184
>50	0.10172347940110162	3.7281218164661776
>100	0.11077179645205006	12.52317671395677
>500	0.012701138868626151	4.489669216232102
>1k	0.004174500187590414	3.3628420840563957
>5k	2.6645745878236686E-4	0.979492453801836
>10k+	8.881915292745562E-5	0.46614204166049183
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10042	0.4649732786218788	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9046	0.4188556341778048	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6990	0.32365696251413395	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5065	0.23452396496911138	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2757	0.12765697362681935	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2753	0.1274717622033492	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2382	0.11029340267649029	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2363	0.10941364841500693	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2300	0.10649656849535166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.26057117350884E-5	0.0
2	0.0	0.0	0.0	9.26057117350884E-5	0.0
3	0.0	0.0	0.0	9.26057117350884E-5	0.0
4	0.0	0.0	0.0	9.26057117350884E-5	0.0
5	0.0	0.0	0.0	9.26057117350884E-5	0.0
6	0.0	0.0	0.0	9.26057117350884E-5	0.0
7	0.0	0.0	0.0	9.26057117350884E-5	0.0
8	0.0	0.0	0.0	9.26057117350884E-5	0.0
9	0.0	0.0	0.0	9.26057117350884E-5	0.0
10	0.0	0.0	0.0	1.389085676026326E-4	0.0
11	0.0	0.0	0.0	2.31514279337721E-4	0.0
12	0.0	0.0	0.0	3.704228469403536E-4	0.0
13	0.0	0.0	0.0	4.1672570280789777E-4	0.0
14	0.0	0.0	0.0	8.334514056157955E-4	0.0
15	0.0	0.0	0.0	0.0012501771084236933	0.0
16	0.0	0.0	0.0	0.0021762342257745774	0.0
17	0.0	0.0	0.0	0.0031948970548605496	0.0
18	0.0	0.0	0.0	0.0036579256135359918	0.0
19	0.0	0.0	0.0	0.004352468451549155	0.0
20	0.0	0.0	0.0	0.00463028558675442	0.0
21	0.0	0.0	0.0	0.006111976974515834	0.0
22	0.0	0.0	0.0	0.008102999776820234	0.0
23	0.0	0.0	0.0	0.011899833957958858	0.0
24	0.0	0.0	0.0	0.01796550807660715	0.0
25	0.0	0.0	0.0	0.02129931369907033	0.0
26	0.0	0.0	0.0	0.027411290673586164	0.0
27	0.0	0.0	0.0	0.04968296434587492	0.0
28	0.0	0.0	0.0	0.09746751160118054	0.0
29	0.0	0.0	0.0	0.15867988705807398	0.0
30	0.0	0.0	0.0	0.24679422177401059	0.0
31	0.0	0.0	0.0	0.4790956496614798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAC	75	2.0683269E-4	17.266666	1
GGTATCA	4835	0.0	16.376423	1
ATCGGTC	80	3.3842877E-4	16.1875	14
AAACCCG	70	0.0025934835	15.857142	5
CGTCGTA	175	2.240995E-9	14.8	10
TATCCCG	75	0.0041059596	14.8	5
TCTAGCG	190	4.5656634E-10	14.605264	28
CTAGCGG	200	9.840733E-10	13.874999	29
GAGTCCG	135	6.5729E-6	13.703703	7
TAGACAG	230	4.0017767E-11	13.673913	5
GTCGCGT	260	1.8189894E-12	13.519231	8
CGGCCTT	760	0.0	13.388158	24
GCGTTAT	235	5.638867E-11	13.382979	1
TCGCCAT	595	0.0	13.369747	13
TTCCTCG	790	0.0	13.348101	19
CTTATAC	1250	0.0	13.32	37
TCGCGTA	265	1.8189894E-12	13.264152	9
TCTCGCC	775	0.0	13.129033	12
TCTTATA	2075	0.0	13.106024	37
CTATCGG	100	0.0018333877	12.949999	12
>>END_MODULE