FastQCFastQC Report
Fri 10 Feb 2017
ERR1630190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330107
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT78940.2370494401531242No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA62780.1885224708995837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51360.15422927851867824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.13014596828270084No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG38040.11423056376266588No Hit
GCTCAGACCAGCTCATACTTCATGCTGATCTCCTGCCTGATGT37910.11384018591594804No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC37380.11224864546394454No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA36210.108735244843484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35420.10636294869804483No Hit
GATTAACACTGCTTGGGGCTCCAATGCCCCAGGACTTGTAGGA34570.10381047816181282No Hit
CCTTAATCAGTGAGGCCACAAAGGCACCCTCGGCCTGGGTGAG34080.10233905397033789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC16500.023.7696973
GTTCTAG17650.022.535411
CTAGACA17450.022.3696254
CTCTAGT17350.021.11239227
GGTATCA22150.020.7968391
GCCTCTA16050.020.28660625
GTCTAGA14250.020.1228071
ACATGTA16650.019.888898
TCTAGTT19200.019.46354328
TAGACAT20000.019.4255
TAGAACT15250.019.2885254
CTAGAAC15300.019.1045743
CTAGTTT19200.019.07812529
GCAATTA14400.018.75694517
CCTCTAG21600.018.67129726
ACTGATC13600.018.6360288
GACATGT18250.018.2465767
CGGAGTC12700.017.91732227
ACAACGG13200.017.6590923
ACGGAGT12800.017.63281226
Produced by FastQC (version 0.11.5)