FastQCFastQC Report
Fri 10 Feb 2017
ERR1630189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394897
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54811.3879568596368166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47871.2122148307026896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32150.8141363444138597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.6092727977168729No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.24006259860166068No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.17548879834488487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.17396941480943132No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.17371618422018906No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6820.17270326186322002No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5600.14180912997566453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.1215506828362839No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4540.11496668751598518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACATT251.2321584E-436.99999626
TGGAATT352.3843677E-531.71428523
CGGAAGG405.930409E-527.7517
GCCGGAA451.3210639E-424.66666615
GGATCAC451.3210639E-424.66666623
CCGCTTA451.3210639E-424.66666625
GCCAGGC551.8985611E-523.54545436
CGTCTGC400.001928955123.12510
CGCCGAC400.001928955123.12525
CTAGCCT400.001928955123.1254
TAGTTGC652.675446E-622.7692327
GTTGCTA652.675446E-622.7692320
TTGATGT753.7279096E-722.24
TTAGTCA502.6977516E-422.19999937
CTAATGA502.6977516E-422.1999994
GCAGTCG855.1590177E-821.7647069
GATCACA603.7183156E-521.58333424
GCTAGTC603.7183156E-521.5833347
TTGCAAG705.087937E-621.14285730
ACGTTTA450.003821427720.55555526
Produced by FastQC (version 0.11.5)