FastQCFastQC Report
Fri 10 Feb 2017
ERR1630188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414810
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48120.1992703359684613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40520.16779788057859624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31500.130445045365888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.019.908631
CGCGTTC951.6780177E-719.47368426
GCGCGTT902.1542146E-618.525
TATATCG1156.413575E-817.6956525
CGAACTA1455.353104E-815.31034424
GTATAGA3750.014.3066661
TCTATAC3950.014.0506323
GACGGAC3000.013.5666667
CACCGGT1102.4581168E-413.4545457
GTATCAA29250.013.4085462
AAGACGG3750.013.325
GCGCATT850.00940865313.05882311
TACGTTA1000.001833496612.94999919
AGACGGA3450.012.8695656
GGCGCGT1307.0040354E-512.80769324
TCTTATA13600.012.78676437
ATATCGC1602.6982962E-612.718756
CGCGGTT3350.012.70149110
TAGGACC5000.012.584
CCGCCTA1359.9688375E-512.33333415
Produced by FastQC (version 0.11.5)