##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630188.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2414810
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861309585433222	33.0	31.0	34.0	30.0	34.0
2	32.078434742277864	33.0	31.0	34.0	30.0	34.0
3	32.21054865600192	34.0	31.0	34.0	30.0	34.0
4	35.83912150438337	37.0	35.0	37.0	35.0	37.0
5	35.76254984864234	37.0	35.0	37.0	35.0	37.0
6	35.81691106132573	37.0	35.0	37.0	35.0	37.0
7	35.79168506010825	37.0	35.0	37.0	35.0	37.0
8	35.794259175670135	37.0	35.0	37.0	35.0	37.0
9	37.543791437007464	39.0	37.0	39.0	35.0	39.0
10	37.432605877895156	39.0	37.0	39.0	34.0	39.0
11	37.498825166369194	39.0	37.0	39.0	35.0	39.0
12	37.41925658747479	39.0	37.0	39.0	34.0	39.0
13	37.43735242110145	39.0	37.0	39.0	34.0	39.0
14	38.68516322195121	40.0	38.0	41.0	35.0	41.0
15	38.695184300214095	40.0	38.0	41.0	35.0	41.0
16	38.64837647682426	40.0	38.0	41.0	34.0	41.0
17	38.61409966001466	40.0	38.0	41.0	34.0	41.0
18	38.57581880147921	40.0	38.0	41.0	34.0	41.0
19	38.605585946720446	40.0	38.0	41.0	34.0	41.0
20	38.57911388473627	40.0	38.0	41.0	34.0	41.0
21	38.53047113437496	40.0	38.0	41.0	34.0	41.0
22	38.494001598469445	40.0	38.0	41.0	34.0	41.0
23	38.44126867124122	40.0	38.0	41.0	34.0	41.0
24	38.40910340772152	40.0	38.0	41.0	34.0	41.0
25	38.389790087004776	40.0	38.0	41.0	34.0	41.0
26	38.22960895474178	40.0	38.0	41.0	34.0	41.0
27	38.114497206819586	40.0	38.0	41.0	33.0	41.0
28	38.05240826400421	40.0	38.0	41.0	33.0	41.0
29	37.99805367710089	40.0	38.0	41.0	33.0	41.0
30	37.93775907835399	40.0	37.0	41.0	33.0	41.0
31	37.885852717191	40.0	37.0	41.0	33.0	41.0
32	37.807072192015106	40.0	37.0	41.0	33.0	41.0
33	37.721827390146636	40.0	37.0	41.0	33.0	41.0
34	37.67032313101238	40.0	37.0	41.0	32.0	41.0
35	37.545735275239046	40.0	37.0	41.0	32.0	41.0
36	37.46554014601563	40.0	37.0	41.0	32.0	41.0
37	37.4042988889395	40.0	37.0	41.0	31.0	41.0
38	37.31362467440503	40.0	36.0	41.0	31.0	41.0
39	37.23648320157694	40.0	36.0	41.0	31.0	41.0
40	37.13862042976466	39.0	36.0	41.0	31.0	41.0
41	37.029033754208406	39.0	36.0	41.0	31.0	41.0
42	36.98936272418948	39.0	36.0	41.0	31.0	41.0
43	35.79496689180515	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	6.0
11	3.0
12	1.0
13	3.0
14	5.0
15	7.0
16	25.0
17	28.0
18	80.0
19	149.0
20	356.0
21	683.0
22	1290.0
23	2145.0
24	3720.0
25	6071.0
26	8978.0
27	13447.0
28	19191.0
29	26710.0
30	35653.0
31	47246.0
32	61391.0
33	79942.0
34	105454.0
35	141464.0
36	197595.0
37	301609.0
38	567608.0
39	793940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34213043676314	19.380075451070685	12.12799350673552	26.149800605430656
2	18.911301510263748	20.630070274679994	34.49426662967273	25.96436158538353
3	19.709004021020288	23.183687329437927	29.447699819033378	27.659608830508404
4	14.595392598175424	15.735979228179442	34.82095899884463	34.8476691748005
5	14.789527954580278	36.13199382146007	34.36821116361122	14.710267060348434
6	34.62052915136181	35.2257113396085	15.681233720251283	14.472525788778412
7	29.37655550540208	30.03727001296168	21.739143038168635	18.847031443467603
8	27.026142843536345	33.63978946583789	19.83381715331641	19.500250537309356
9	26.48448532182656	14.153411655575388	19.88471142657186	39.47739159602619
10	16.962742410375974	26.36571821385533	33.54450246603252	23.12703690973617
11	36.08022991456885	21.785647732119713	21.527780653550384	20.606341699761057
12	21.321056314989583	25.41963964038579	29.665605161482684	23.593698883141943
13	30.059756254115232	19.96956282274796	24.723063098131945	25.247617825004866
14	22.66016788070283	20.637731332899897	25.091208003942338	31.610892782454936
15	25.222522682944	27.898219735714196	21.95555758009947	24.92370000124233
16	25.034474761989557	26.181066005192953	24.125956079360282	24.658503153457207
17	23.625378394159373	26.36580103610636	25.560561700506458	24.448258869227807
18	23.93401551260762	25.48693271934438	26.301738024937784	24.277313743110223
19	24.332514773419025	25.36203676479723	26.381288797048214	23.924159664735527
20	24.332266306665947	25.38435736144873	26.18777460752606	24.095601724359266
21	24.673493980892907	25.347874159871793	25.889241803702983	24.08939005553232
22	24.702564591003018	25.244263523838313	25.966804841788793	24.086367043369872
23	24.3062601198438	25.24393223483421	26.130254554188525	24.319553091133464
24	24.22099461241257	25.7859210455481	25.718793611091556	24.274290730947776
25	24.742526327123045	25.163967351468646	25.98622665965438	24.10727966175393
26	24.420844704138215	25.75527681266849	25.770557517982784	24.053320965210514
27	24.344192710813687	25.416906506101927	25.74554519817294	24.49335558491144
28	24.290979414529506	25.380671771278074	26.179658026925512	24.148690787266908
29	24.00482853723481	25.574020316298178	26.406508172485623	24.01464297398139
30	23.897739366658246	25.9444842451373	26.057453795536713	24.100322592667748
31	24.433806386423775	25.531366857019805	25.905350731527534	24.129476025028886
32	23.85111043933063	25.563419068166855	26.07861488067384	24.506855611828673
33	23.71449513626331	25.423408052807467	26.391434522798896	24.470662288130328
34	24.446395368579722	25.00515568512637	26.123504540729915	24.424944405564
35	24.270108207270965	25.281367892297947	26.573850530683572	23.87467336974752
36	24.074730517100722	25.472273180912786	25.947921368554876	24.50507493343162
37	24.157677001503224	24.82572127827862	26.53902377412716	24.477577946090996
38	23.776280535528674	25.120816958684124	26.716304802448228	24.386597703338982
39	23.645131501029066	24.934798182879813	27.01794344068477	24.402126875406346
40	23.654531826520515	24.783440519129872	27.390063814544412	24.1719638398052
41	23.188118319867815	24.80207552561071	27.547219035866178	24.4625871186553
42	23.26986388163044	25.002132672963917	27.513096268443483	24.214907176962164
43	23.212840761799065	24.35835531573913	27.60589860071807	24.82290532174374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	239.0
1	260.5
2	282.0
3	609.5
4	937.0
5	937.0
6	1254.0
7	1571.0
8	1408.5
9	1246.0
10	1780.5
11	2315.0
12	2315.0
13	4140.0
14	5965.0
15	8252.0
16	10539.0
17	10843.5
18	11148.0
19	11148.0
20	14494.5
21	17841.0
22	18524.5
23	19208.0
24	23479.0
25	27750.0
26	27750.0
27	32690.0
28	37630.0
29	45090.0
30	52550.0
31	61286.0
32	70022.0
33	70022.0
34	81231.0
35	92440.0
36	102682.5
37	112925.0
38	122942.5
39	132960.0
40	132960.0
41	143062.5
42	153165.0
43	158344.0
44	163523.0
45	167289.5
46	171056.0
47	171056.0
48	173060.0
49	175064.0
50	175772.5
51	176481.0
52	174041.5
53	171602.0
54	171602.0
55	165741.5
56	159881.0
57	152109.0
58	144337.0
59	137441.0
60	130545.0
61	130545.0
62	119808.0
63	109071.0
64	96686.5
65	84302.0
66	73085.0
67	61868.0
68	61868.0
69	53068.0
70	44268.0
71	36861.0
72	29454.0
73	23602.5
74	17751.0
75	17751.0
76	14107.5
77	10464.0
78	8286.0
79	6108.0
80	4889.0
81	3670.0
82	3670.0
83	2774.0
84	1878.0
85	1555.0
86	1232.0
87	1040.0
88	848.0
89	848.0
90	693.0
91	538.0
92	308.0
93	78.0
94	59.5
95	41.0
96	41.0
97	26.5
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2414810.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.89657129743498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49571560427714	47.183225089185164
2	10.740632638074082	12.436916066196266
3	3.1542269584336875	5.478567779617425
4	1.4186722467278585	3.2854503552148695
5	0.7477383725497907	2.1645743999078477
6	0.48950687628739203	1.700446185813461
7	0.3101628433086799	1.2570155620005297
8	0.2307292325317729	1.0686745169342597
9	0.18038271145200557	0.9399186462965115
>10	1.0480097348135007	11.577623426880768
>50	0.11447376728805732	4.640130663335171
>100	0.0671714543250218	6.839971447581999
>500	0.0022195654962292874	0.8027431670044464
>1k	3.579944348753524E-4	0.6247426940312176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4812	0.1992703359684613	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4052	0.16779788057859624	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3150	0.130445045365888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.141112551298032E-5	8.282225102596064E-5
2	0.0	0.0	0.0	4.141112551298032E-5	8.282225102596064E-5
3	0.0	0.0	0.0	4.141112551298032E-5	8.282225102596064E-5
4	0.0	0.0	0.0	4.141112551298032E-5	8.282225102596064E-5
5	0.0	0.0	0.0	4.141112551298032E-5	1.2423337653894097E-4
6	0.0	0.0	0.0	4.141112551298032E-5	1.6564450205192128E-4
7	0.0	0.0	0.0	4.141112551298032E-5	1.6564450205192128E-4
8	0.0	0.0	0.0	4.141112551298032E-5	1.6564450205192128E-4
9	0.0	0.0	0.0	4.141112551298032E-5	1.6564450205192128E-4
10	0.0	0.0	0.0	4.141112551298032E-5	2.070556275649016E-4
11	0.0	0.0	0.0	4.141112551298032E-5	2.070556275649016E-4
12	0.0	0.0	0.0	4.141112551298032E-5	2.070556275649016E-4
13	0.0	0.0	0.0	4.141112551298032E-5	2.070556275649016E-4
14	0.0	0.0	0.0	4.141112551298032E-5	2.070556275649016E-4
15	0.0	0.0	0.0	2.070556275649016E-4	2.070556275649016E-4
16	0.0	0.0	0.0	2.4846675307788193E-4	3.3128900410384256E-4
17	0.0	0.0	0.0	3.3128900410384256E-4	3.3128900410384256E-4
18	0.0	0.0	0.0	3.7270012961682287E-4	4.555223806427835E-4
19	0.0	0.0	0.0	4.969335061557639E-4	4.555223806427835E-4
20	0.0	0.0	0.0	5.383446316687442E-4	4.969335061557639E-4
21	0.0	0.0	0.0	7.039891337206654E-4	9.524558867985473E-4
22	0.0	0.0	0.0	9.524558867985473E-4	9.524558867985473E-4
23	0.0	0.0	0.0	0.0014493893929543112	9.524558867985473E-4
24	0.0	0.0	0.0	0.0022776119032139174	9.524558867985473E-4
25	0.0	0.0	0.0	0.003023012162447563	9.524558867985473E-4
26	0.0	0.0	0.0	0.005383446316687441	9.524558867985473E-4
27	0.0	0.0	0.0	0.015280705314289737	0.001035278137824508
28	0.0	0.0	0.0	0.03900928023322746	0.001035278137824508
29	0.0	0.0	0.0	0.07375321453861794	0.001035278137824508
30	0.0	0.0	0.0	0.11599256256185787	0.001035278137824508
31	0.0	0.0	0.0	0.2552995887875237	0.001035278137824508
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1970	0.0	19.90863	1
CGCGTTC	95	1.6780177E-7	19.473684	26
GCGCGTT	90	2.1542146E-6	18.5	25
TATATCG	115	6.413575E-8	17.695652	5
CGAACTA	145	5.353104E-8	15.310344	24
GTATAGA	375	0.0	14.306666	1
TCTATAC	395	0.0	14.050632	3
GACGGAC	300	0.0	13.566666	7
CACCGGT	110	2.4581168E-4	13.454545	7
GTATCAA	2925	0.0	13.408546	2
AAGACGG	375	0.0	13.32	5
GCGCATT	85	0.009408653	13.058823	11
TACGTTA	100	0.0018334966	12.949999	19
AGACGGA	345	0.0	12.869565	6
GGCGCGT	130	7.0040354E-5	12.807693	24
TCTTATA	1360	0.0	12.786764	37
ATATCGC	160	2.6982962E-6	12.71875	6
CGCGGTT	335	0.0	12.701491	10
TAGGACC	500	0.0	12.58	4
CCGCCTA	135	9.9688375E-5	12.333334	15
>>END_MODULE