FastQCFastQC Report
Fri 10 Feb 2017
ERR1630187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323564
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46320.349964187602564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38540.29118350151560485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29110.21993647454902066No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG27250.2058835084665343No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG26770.20225693657427973No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG23560.17800423704482746No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC23420.17694648690958656No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT23170.17505764738237065No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA19680.14868944758243652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.14234294677099105No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC18550.1401518929194206No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA17840.13478758866212742No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT17700.1337298385268865No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA17160.12964994514810013No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC17000.12844108785068195No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG16080.1214901583905274No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA15520.11725915784956377No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT15440.11665472920085466No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG15290.11552142548452511No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC14840.11212151433553648No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA14760.11151708568682737No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC14730.11129042494356148No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC14560.11000601406505464No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA14390.10872160318654783No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG13830.1044906026455842No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG13820.10441504906449556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG500.00703543118.534
AATGCGT1054.7986214E-717.61904735
GGTATCA18150.017.4297541
ATCGGCT650.001580104817.07692325
CGCAAGA1950.017.0769232
ATGCGTT1107.8097946E-716.81818236
TCTAGCG1352.2200766E-816.44444528
ACCCGTT904.446775E-516.44444530
TGCGTTA1251.6581544E-716.27999937
GTACTAC1151.2424662E-616.0869561
TTAGTCG700.002592771515.8571435
GACGGAC2003.6379788E-1215.7257
TACGTTA1302.5896225E-715.65384619
GTATAGA1201.9359413E-615.4166671
CTAGCGG1455.3467375E-815.31034529
CGGTTAC1001.0935624E-414.822
CACAACG1001.0935624E-414.817
ACGGTTA1001.0935624E-414.821
TAGACGG750.00410484414.7999992
CCCCGTA750.00410484414.79999915
Produced by FastQC (version 0.11.5)